Engineering a chromatin looping factor for artificial gene regulation

设计用于人工基因调控的染色质环因子

• 批准号: DP190102543
• 负责人: Prof Jacqueline Matthews
• 金额: $ 37.93万
• 依托单位: The University of Sydney
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2019
• 资助国家: 澳大利亚
• 起止时间: 2019-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP190102543
• 关键词: Engineering; chromatin; looping; factor; artificial

This project aims to define mechanisms of chromatin looping and gene activation by a widely expressed mammalian protein. The project will establish if the functions of this protein are modulated by the binding of small molecules, whether it can act in conjunction with closely related proteins, and if post-translational modifications regulate looping and gene activation. Using protein engineering the project will develop synthetic looping factors that can switch on a wide array of target genes. The project aims to answer fundamental questions about how proteins can establish and maintain physical loops in DNA to modulate gene expression. The project will also develop research tools that might ultimately correct diseases caused by the faulty expression of genes.

本项目旨在通过广泛表达的哺乳动物蛋白来确定染色质环和基因激活的机制。该项目将确定该蛋白的功能是否受到小分子结合的调节，是否可以与密切相关的蛋白质结合，以及翻译后修饰是否调节环和基因激活。利用蛋白质工程技术，该项目将开发出能够开启大量靶基因的合成环因子。该项目旨在回答有关蛋白质如何在DNA中建立和维持物理环以调节基因表达的基本问题。该项目还将开发研究工具，最终可能纠正由基因表达错误引起的疾病。