Silica-protein nanocomposites for dental repair

描述（由申请人提供）：主要需要能够改善颅面组织和牙齿缺损再生的生物材料，其中可以定制界面和成骨的控制。具体而言，骨再生和钛-骨界面是界面结合和骨重建需要改进的两个关键领域。在这项更新提案中，我们建立在当前赠款的基础上，我们为基于丝-二氧化硅纳米复合材料的高度定制的骨再生生物材料新家族奠定了基础。具体而言，基于生物工程和化学方法的有机（丝）和无机（二氧化硅）结构域的控制，以及随后在骨再生方面的成功，为这种更新奠定了基础。我们的假设是，这种生物工程方法的生物材料设计，特别是有机-无机纳米复合材料系统，可以利用对骨组织再生的多功能生物材料系统的设计。化学、序列、组装和材料功能（成骨）的严格控制可以使用这种方法来定制。我们计划通过添加选择性骨结合肽和钛结合肽来优化界面，并包括抗菌成分来扩展这种新的嵌合蛋白家族的功能特征。这些新系统将在体外评估与hMSCs成骨标志物和机制相关的，然后在体内动物模型中探索骨界面，骨形成和骨中的钛锚定，作为颅面和牙科领域的关键需求。的能力 定制这种高度受控的多功能生物材料的化学和结构以调节二氧化硅相的尺寸和形态，允许形成颅面和牙齿修复方案中所需的纳米级复合材料。我们计划用新功能扩展这些系统，为与二氧化硅组分相一致的改进界面提供一条新的前进道路，从而实现一系列新的生物材料支架设计，以满足颅面和牙齿修复需求。由于生物工程方法中嵌入的设计特征，这些新型嵌合蛋白生物材料的所有特征的前所未有的控制对用于组织界面和组织再生的广泛的新型生物材料具有影响。

DESCRIPTION (provided by applicant): There is a major need for biomaterials that provide improved regeneration of craniofacial tissue and dental defects, where control of interfaces and osteogenesis can be tailored. Specifically, bone regeneration and titanium-bone interfaces are two key areas where improvements in interfacial bonding and bone remodeling are in need of advancements. In this renewal proposal, we build upon our progress in the current grant where we established the foundation for a new family of highly tailored biomaterials for bone regeneration based on silk-silica nanocomposites. Specifically, control of the organic (silk) and inorganic (silica) domains, based on bioengineering and chemistry approaches, and subsequent success in bone regeneration, lays the groundwork for this renewal. Our hypothesis is that this bioengineering approach to biomaterial design, and in particular organic-inorganic nanocomposite systems, can be exploited towards the design of multifunctional biomaterial systems for bone tissue regeneration. Tight control of chemistry, sequence, assembly and material functions (osteogenesis) can be tailored using this approach. We plan to expand the functional features of this new family of chimeric proteins by adding selective bone binding and titanium binding peptides to optimize interfaces, and also include antimicrobial components. These new systems will be evaluated in vitro related to osteogenic markers from hMSCs and mechanisms, and then in vivo in animal models to explore bone interfaces, bone formation and titanium anchoring in bone, as critical needs in the craniofacial and dental fields. The ability to tailor the chemistry and structure of such highly controlled multifunctional biomaterials to regulate the size and morphology of the silica phase, allows the formation of nano-scale composites required in craniofacial and dental repair scenarios. We plan to expand these systems with new functions to provide a novel path forward for improved interfaces in concert with the silica components, such that a new family of biomaterial scaffold designs will be achieved to match craniofacial and dental repair needs. The unprecedented control of all features of these novel chimeric protein biomaterials, due to the design features embedded in the bioengineering approach, has implications for a wide range of new biomaterials for tissue interfaces and tissue regeneration.