Tissue Engineering Resource Center

组织工程资源中心

• 批准号: 10213714
• 负责人: DAVID L. KAPLAN
• 金额: $ 32.78万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-16 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10213714
• 关键词: 3D Print; Biocompatible Materials; Biological; Biological Process; Biology; Biomedical Engineering; Biopolymers; Biotin; Carbodiimides; Cells; Chemistry; Cicatrix; Collaborations; Collagen; Complex; Coupling; Crystallization; Deposition; Devices; Disease model; Elastin; Environment; Enzymes; Extracellular Matrix; Fiber; Fibrosis; Free Radicals; Freeze Drying; Gel; Genetic Engineering; Goals; Higher Order Chromatin Structure; Hyaluronic Acid; Immune response; In Vitro; Inflammation; Light; Mechanics; Mediating; Microfluidics; Modification; Natural regeneration; Outcome; Oxygen; Polymers; Polysaccharides; Property; Reaction; Reactive Oxygen Species; Regenerative Medicine; Resources; Series; Signal Transduction; Silk; Site; Source; Stimulus; Streptavidin; Structural Protein; Structure; Study models; System; Temperature; Therapeutic; Time; Tissue Engineering; Tissue Model; Tissues; Variant; Vascularization; aqueous; base; biomaterial compatibility; copolymer; crosslink; cytokine; design; dynamic system; electric field; experience; functional restoration; improved; in vivo; mechanical properties; monomer; nerve supply; preservation; public health relevance; regeneration model; response; restoration; scaffold; self assembly; tissue regeneration; tissue support frame

SUMMARY TRD1 will focus on the design and implementation of adaptive-responsive biomaterials to meet the goals of the P41. These are biomaterials that can sense and actuate cells or respond to the local environment to drive the functional restoration of complex tissue structures, in vitro and in vivo. The hypothesis is that biomaterial systems with integrated features for activation/response can provide specific benefits for designing scaffolds for restoration of tissue structure and function. These new material systems will be integrated with the needs and goals of the other TRDs to optimize tissue outcomes at both the fundamental and translational levels. The ultimate goal is to develop biomaterial systems for functional restoration of complex tissue structures based on the response to changes in the local environment (intrinsic signaling) or via applied changes (extrinsic signaling). The plans are to develop materials that provide adaptive responses to changes in local biology and conditions (e.g., pH, temperature reactive oxygen, enzymes) or to external signals (e.g., light, electric fields) to effect a change to improve tissue function or regeneration goals. Control of mechanical properties, degradation and release of bioactive factors to respond to local changes are examples of dynamic response-control goals. The core biomaterials will be based on biopolymers (e.g., elastins, collagens, silks, hyaluronic acid, others) as bioengineered variants and composites. Three specific aims will be pursued. Aim 1: Biomaterials that dynamically change properties in response to local signals. These biopolymer systems will provide core functions for sensing-response using silk-elastin chemistry and composite designs with hyaluronic acid and collagen, for a broad range of utility for different cell and tissue needs. Aim 2: Biomaterials that change properties on demand, in response to external stimuli. The focus will be on light activation systems (via specific chemistries) or electric field-mediated changes (via incorporated conductive components). The goal is to control the material volume, mechanics, and delivery of bioactive factors in an on-demand mode, using external sources (light, electric field), to control cell and tissue outcomes. Aim 3: “Smart” scaffolds for tissue regeneration and modeling of disease. Scaffold designs based on the materials from Aims 1 and 2 will be utilized to generate functional devices and tissue models for studies in TRD1, 2 and 3, in vitro and in vivo.

总结 TRD 1将专注于自适应响应生物材料的设计和实施，以满足 P41的目标。这些生物材料可以感知和驱动细胞或对局部 环境来驱动体外和体内复杂组织结构的功能恢复。的 假设具有激活/响应的集成特征的生物材料系统可以提供 设计用于恢复组织结构和功能的支架的具体益处。这些新 材料系统将与其他TRD的需求和目标相结合，以优化组织 在基础和转化层面上的成果。最终目标是开发生物材料 基于对组织结构变化的响应的复杂组织结构的功能恢复系统 局部环境（内在信号）或通过应用的变化（外在信号）。计划是 开发对当地生物和条件变化提供适应性反应的材料（例如，pH值、 温度活性氧，酶）或外部信号（例如，光，电场）来实现 改变以改善组织功能或再生目标。机械性能、降解的控制 和释放生物活性因子以响应局部变化是动态响应控制的例子 目标.核心生物材料将基于生物聚合物（例如，弹性蛋白，胶原蛋白，丝，透明质酸 酸等）作为生物工程变体和复合物。将追求三个具体目标。目标1： 响应局部信号动态改变特性的生物材料。这些生物聚合物 系统将使用丝弹性蛋白化学和复合材料提供传感响应的核心功能 透明质酸和胶原蛋白的设计，用于不同细胞和组织需求的广泛用途。 目标2：生物材料根据需求改变特性，以响应外部刺激。的 重点将放在光激活系统（通过特定的化学物质）或电场介导的变化（通过 结合的导电部件）。目标是控制材料体积、力学和 使用外部源（光、电场）以按需模式递送生物活性因子， 控制细胞和组织的结果。目标3：用于组织再生和建模的“智能”支架 疾病基于目标1和2材料的支架设计将用于生成 用于研究RD 1、2和3的体外和体内功能器械和组织模型。