3D Intestinal Tissues

3D 肠道组织

• 批准号: 9312411
• 负责人: DAVID L. KAPLAN
• 金额: $ 30.55万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-07 至 2022-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9312411
• 关键词: Address; Area; Biocompatible Materials; Biological; Biological Assay; Bioreactors; Cells; Clinic; Coculture Techniques; Colon; Communicable Diseases; Complex; Data; Development; Disease; Engineering; Enteral; Extracellular Matrix; Faculty; Frequencies; Goals; Human; Immune; Individual; Infection; Intestines; Laboratories; Mechanics; Modeling; Modification; Molds; Organoids; Outcome; Oxygen; Perfusion; Peristalsis; Pharmaceutical Preparations; Phase; Physiological; Population; Postdoctoral Fellow; Preparation; Quality Control; Reproducibility; Research; Research Project Grants; Robotics; Scaffolding Protein; Series; Silk; Small Intestines; Smooth Muscle; Source; Supplementation; Supporting Cell; System; Tissue Engineering; Tissue Model; Tissue Sample; Tissues; Training; Validation; Variant; Work; base; cell type; insight; pathogen; response; scaffold; scale up; systems research; tissue support frame

Core B – Summary Core B will utilize the improvements developed for the 3D intestinal systems to support the preparation and utility of the tissue models, including generating the scaffolds and seeded scaffolds/tissues for all projects. The plans are based on our extensive preliminary data where we have demonstrated structurally- and functionally- relevant 3D human intestinal tissues for the study of infectious diseases. The intestinal systems include enteroid primary cell sources, a 3D porous protein scaffolding system with a geometrically-engineered hollow lumen with adaptability to both large and small intestinal tissues. In Aim #1 the focus will be on the preparation of scaffolds and 3D intestinal systems for the three research projects. Here the goal is to provide intestinal tissues to support the three research projects (60 systems per week), obtain primary tissue samples from the clinic, establish early passage organoid material from both the small intestine and the colon, and train individuals in the various projects. In addition, a focus will be on documenting all materials for validation and quality control to assure reproducibility. In Aim #2 the focus will be on optimization of the 3D intestinal tissues. Here the goal is to continue to optimize the currently developed 3D human intestinal tissues generated from organoids for physiological relevance, and develop increasingly complex features to facilitate analysis of intestinal pathogens. These activities will include the incorporation of additional cell types, supplementation of the scaffolds with ECM components, and further development of a bioreactor system to control flow, peristalsis and oxygen levels. A team of faculty, post doctoral fellows and a technician will support the needs of Core B and work closely with the research teams on all phases of the plans.

核心B - 摘要 核心B将利用为3D肠系统开发的改进来支持准备和 组织模型的效用，包括为所有项目生成支架和种子支架/组织。这 计划基于我们广泛的初步数据，我们在结构和功能上证明了 相关的3D人肠组织用于研究传染病。肠系统包括 Enteroid Orimal细胞源，一种3D多孔蛋白脚手架系统，具有几何设计的空心 对大小肠组织适应性的管腔。在AIM＃1中，重点将放在准备上 三个研究项目的脚手架和3D肠系统。这里的目标是提供肠道 组织支持三个研究项目（每周60个系统），从 诊所，从小肠和结肠建立早期通道的器官材料，然后训练 此外，将重点放在记录所有验证材料和 质量控制以确保可重复性。在AIM＃2中，重点将是优化3D肠组织。 在这里，目标是继续优化当前开发的3D人类肠道组织 身体相关性的器官，并发展越来越复杂的特征，以促进分析 肠道病原体。这些活动将包括其他单元类型的清单，补充 带有ECM组件的脚手架，并进一步开发生物反应器系统以控制流动，蠕动 和氧气水平。一支由教师，博士后研究员和技术人员的团队支持核心B的需求 并与研究团队紧密合作计划的所有阶段。