Evaluation and Validation of Dried Blood Spots as Matrix for Exposure Assessment

描述(申请人提供)：生物监测已成为评估接触包括持久性有机污染物(POPs)在内的各种化学品的常用工具。持久性有机污染物通常是在血清中测量的，通常需要抽取10毫升或更多的全血。由于样本收集的侵入性，婴儿和儿童通常不包括在评估持久性有机污染物暴露或健康影响的研究中。干血点(DBS)是从所有新生儿出生时收集的，并由国家卫生部门存档多年。DBS是持久性有机污染物生物监测的潜在基质。使用DBS的明显优势包括：(1)可以很容易地从参与者或非医务人员那里收集；(2)与血液采集用品相比，DBS卡很便宜；(3)每个州都有一个来自该州出生的所有儿童的DBS档案；(4)DBS可以通过标准的邮政服务轻松地从现场邮寄到实验室；以及(5)可以用来采集婴儿或儿童的血液样本。尽管它们具有优势，但很少有实验室尝试将它们用作生物监测研究的基质。我们建议建立并验证一种灵敏、选择性和精确度高的方法来同时测定DBS中的多溴联苯、多氯联苯和有机氯农药。我们将在密歇根州1970-1980年存档的50个新生儿DBS样本中测量这些化学物质，密歇根州在10世纪70年代初经历了一次污染事件，导致广泛接触某些持久性有机污染物。这些测量将帮助我们了解存档超过40年的DBS是否有助于历史测试目的，并将帮助我们精确确定污染发生的确切年份。此外，我们计划现场测试分别在干冰上(隔夜)和通过USPS运往实验室的配对血清和DBS样本的预期收集，以确定它们是否存在相关性而没有明显的偏差。这项研究不仅将为我们目前在密歇根州进行的队列研究提供信息，还将为寻求也可用于婴儿和儿童的成本效益样本收集策略的其他研究提供信息。

DESCRIPTION (provided by applicant): Biomonitoring has become a common tool in assessing exposure to a variety of chemicals including persistent organic pollutants (POPs). POPs are typically measured in blood serum, usually requiring a 10 mL whole blood draw or more. Because of the invasiveness of sample collection, infants and children are not typically included in studies evaluating POPs exposure or health effects. Dried blood spots (DBS) are collected from all neonates at birth and are archived by the state health department for a number of years. DBS represent a potential matrix for biomonitoring of POPs. Distinct advantages of using DBS include: (1) they can be easily collected from participant or non-medical personnel; (2) DBS cards are inexpensive compared to blood collection supplies; (3) each state has an archive of DBS from all children born in the state; (4) DBS can be easily mailed from the field to laboratory using standard postal services; and (5) they can be used to collect infant or child blood samples. Despite their advantages, few laboratories have attempted to use them as a matrix for biomonitoring studies. We propose to develop and validate a sensitive, selective and precise method for measuring polybrominated biphenyls, polychlorinated biphenyls and organochlorine pesticides in a single DBS. We will measure these chemicals in 50 neonate DBS samples archived from 1970-1980 in Michigan, a state that experienced a contamination event in the early 1070s which resulted in widespread exposure to certain POPs. These measurements will help us to understand if DBS archived for over 40 years are useful for historic testing purposes and will help us pinpoint the exact year the contamination occurred. In addition, we plan to field test the prospective collection of paired serum and DBS samples shipped to the laboratory on dry ice (overnight) and via USPS, respectively, to determine if they correlate without appreciable bias. This research will not only inform our current cohort study in Michigan but also other studies seeking cost-effective sample collection strategies that can also be used on infants and children.