Evaluation and Validation of Dried Blood Spots as Matrix for Exposure Assessment

干血斑作为暴露评估基质的评估和验证

• 批准号: 8842996
• 负责人: Dana Boyd Barr
• 金额: $ 19.4万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-05-01 至 2017-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8842996
• 关键词: Archives; Back; Biological Monitoring; Birth; Blood; Blood specimen; Body Burden; Chemicals; Child; Cohort Studies; Collection; Communities; Community Health; Consumption; Dermal; Development; Dichlorodiphenyl Dichloroethylene; Dieldrin; Dry Ice; Endocrine disruption; Enrollment; Evaluation; Event; Exposure to; Fees; Female; Flame Retardants; Funding; Future; Gas Chromatography; Genitourinary system; Health; Human Resources; Individual; Industrial Accidents; Infant; Isotopes; Laboratories; Livestock; Location; Measurement; Measures; Methods; Michigan; Modeling; Participant; Pilot Projects; Plants; Plasma; Polybrominated Biphenyls; Polychlorinated Biphenyls; Postal Service; Pregnancy; Registries; Relative (related person); Research; Risk; Sampling; Serum; Services; Shipping; Ships; Soil; Spontaneous abortion; Spottings; Techniques; Testing; Time; Training; United States National Institutes of Health; Validation; Whole Blood; Work; base; beef; biobank; cost effective; dichlorodiphenyltrichloroethane; experience; exposure pathway; feeding; male; maternal serum; neonate; organochlorine pesticide; pollutant; prenatal; prospective; sample collection; tandem mass spectrometry; tool

DESCRIPTION (provided by applicant): Biomonitoring has become a common tool in assessing exposure to a variety of chemicals including persistent organic pollutants (POPs). POPs are typically measured in blood serum, usually requiring a 10 mL whole blood draw or more. Because of the invasiveness of sample collection, infants and children are not typically included in studies evaluating POPs exposure or health effects. Dried blood spots (DBS) are collected from all neonates at birth and are archived by the state health department for a number of years. DBS represent a potential matrix for biomonitoring of POPs. Distinct advantages of using DBS include: (1) they can be easily collected from participant or non-medical personnel; (2) DBS cards are inexpensive compared to blood collection supplies; (3) each state has an archive of DBS from all children born in the state; (4) DBS can be easily mailed from the field to laboratory using standard postal services; and (5) they can be used to collect infant or child blood samples. Despite their advantages, few laboratories have attempted to use them as a matrix for biomonitoring studies. We propose to develop and validate a sensitive, selective and precise method for measuring polybrominated biphenyls, polychlorinated biphenyls and organochlorine pesticides in a single DBS. We will measure these chemicals in 50 neonate DBS samples archived from 1970-1980 in Michigan, a state that experienced a contamination event in the early 1070s which resulted in widespread exposure to certain POPs. These measurements will help us to understand if DBS archived for over 40 years are useful for historic testing purposes and will help us pinpoint the exact year the contamination occurred. In addition, we plan to field test the prospective collection of paired serum and DBS samples shipped to the laboratory on dry ice (overnight) and via USPS, respectively, to determine if they correlate without appreciable bias. This research will not only inform our current cohort study in Michigan but also other studies seeking cost-effective sample collection strategies that can also be used on infants and children.

描述（由申请人提供）：生物监测已成为评估暴露于多种化学物质在内的各种化学物质（包括持续性有机污染物（POPS））的常见工具。 POP通常在血清中测量，通常需要10毫升全血或更多。由于采集样本的侵入性，婴儿和儿童通常不包括在评估POPS暴露或健康影响的研究中。干血点（DB）是从所有新生儿收集的，并由州卫生部门存档多年。 DBS代表了POP生物监测的潜在矩阵。使用DBS的独特优势包括：（1）可以轻松地从参与者或非医学人员那里收集它们； （2）与血液收集用品相比，DBS卡是便宜的； （3）每个州都有来自该州所有儿童的DB档案； （4）使用标准邮政服务可以轻松地将DBS从现场邮寄到实验室； （5）它们可用于收集婴儿或儿童血样。尽管有优势，但很少有实验室试图将其用作生物监测研究的矩阵。我们建议开发和验证一种敏感，选择性和精确的方法，用于测量单个DBS中的多溴二苯基，多氯联苯和有机氯农药。我们将在1970 - 1980年在密歇根州存档的50个新生儿DBS样品中测量这些化学物质，该州在1070年代初期经历了污染事件，导致某些流行音乐的广泛暴露。这些测量将帮助我们了解已存档超过40年的DBS对于历史测试目的有用，并将帮助我们确定污染的确切年份。此外，我们计划现场测试前瞻性收集的配对血清和DBS样品，分别在干冰上运送到实验室（过夜）和通过USPS，以确定它们是否相关而没有明显的偏见。这项研究不仅将为我们当前在密歇根州的队列研究提供信息，而且还将为寻求具有成本效益的样本收集策略的其他研究提供信息，这些研究也可以用于婴儿和儿童。