Impact of prenatal insecticide exposure on neurodevelopmental trajectories in a Thai birth cohort: building exposure science and neurodevelopmental research capacity in Thailand

项目摘要 在发展中国家，接触杀虫剂是普遍存在的， 在整个怀孕期间的活动都是经常接触的。 虽然在一些出生队列研究中， 美国已经显示出产前有机磷（OP）对神经发育的持续影响， 杀虫剂暴露，神经发育途径和暴露时间导致不利的 结果并不清楚。 因此，监管机构一直不愿改变风险评估 标准或提供关于孕妇接触的具体建议。 拟议 泰国的纵向出生队列研究将：1）建立泰国研究人员的能力， 农药相关神经毒性创新性和竞争性研究2）测定OP代谢产物 和拟除虫菊酯（PYR）杀虫剂，以及3）评估以下杀虫剂的影响 产前OP（和PYR作为探索性分析）杀虫剂暴露对神经发育 从出生到3岁的轨迹。 与朱拉隆功大学（CU）合作， 泰国和泰国北部北方的清迈大学（CMU），300名孕妇将在 招募的将采集8份连续尿样、4份连续血样和脐带血， 在怀孕期间收集，以记录在怀孕期间 三个月Brazelton神经行为评估量表将在 出生后进行视觉注意力、情绪调节、记忆和抑制控制测试 以确定神经发育的“级联”如何最终影响整体认知能力， 功能（例如，Bayley-III）。能力建设活动包括开发核心分析工具， CMU的实验室设施和CU的神经发育设施沿着促进 论文写作和国际科学出版物。

Project Summary Exposure to insecticides is ubiquitous in the developing world, where women who perform farming activities throughout pregnancy are routinely exposed. Although several birth cohort studies in the U.S. have shown persistent neurodevelopmental effects of prenatal organophosphate (OP) insecticide exposure, the neurodevelopmental pathways and timing of exposure leading to adverse outcomes is not clear. Thus, regulatory agencies have been reluctant to change risk assessment standards or provide specific recommendations about exposures to pregnant women. The proposed longitudinal birth cohort study in Thailand will: 1) build capacity of Thai researchers to develop innovative and competitive studies of pesticide related neurotoxicity 2) measure metabolites of OP and pyrethroid (PYR) insecticides during each trimester of pregnancy, and 3) evaluate the impact of prenatal OP (and PYR as exploratory analysis) insecticide exposure on neurodevelopmental trajectories from birth to age 3. In collaboration with Chulalongkorn University (CU) in central Thailand and Chiang Mai University (CMU) in northern Thailand, 300 pregnant women will be recruited. Eight serial urine samples, 4 serial blood samples, and umbilical cord blood will be collected during pregnancy to document temporally-resolved insecticide exposure during each trimester. The Brazelton Neurobehavioral Assessment Scale will be administered to offspring at birth followed by tests of visual attention, regulation of emotion, memory and inhibitory control to determine how the “cascade” of neurologic development ultimately affects overall cognitive function (e.g., Bayley-III). Capacity building activities include development of core analytic laboratory facilities at CMU and neurodevelopmental facilities at CU along with courses to promote grant writing and international scientific publications.