Impact of prenatal insecticide exposure on neurodevelopmental trajectories in a Thai birth cohort: building exposure science and neurodevelopmental research capacity in Thailand

项目摘要 在发展中国家，接触杀虫剂是无处不在的，在那里从事耕作的妇女 怀孕期间的活动经常被曝光。尽管有几项出生队列研究 美国已显示出产前有机磷(OP)对神经发育的持续影响 杀虫剂暴露、神经发育途径和暴露导致不良反应的时间 结果尚不清楚。因此，监管机构一直不愿改变风险评估。 标准或提供关于孕妇暴露的具体建议。建议数 泰国的纵向出生队列研究将：1)建立泰国研究人员开发的能力 农药相关神经毒性的创新性和竞争性研究2)OP代谢物的测定 和拟除虫菊酯(PYR)杀虫剂在怀孕的每个三个月，以及3)评估 产前OP(以PYR为探索性分析)杀虫剂暴露对神经发育的影响 从出生到3岁的轨迹。与中部的朱拉隆功大学(CU)合作 泰国和泰国北部的清迈大学(CMU)将有300名孕妇 被招募了。8个连续尿样，4个连续血液样本，以及脐带血 在怀孕期间收集，以记录在每个 三个月。布拉斯尔顿神经行为评定量表将在 分娩后进行视觉注意、情绪调节、记忆和抑制控制测试 来确定神经学发展的“级联”最终如何影响整体认知 功能(例如，贝利-III)。能力建设活动包括制定核心分析 CMU的实验室设施和CU的神经发育设施以及促进 资助写作和国际科学出版物。

Project Summary Exposure to insecticides is ubiquitous in the developing world, where women who perform farming activities throughout pregnancy are routinely exposed. Although several birth cohort studies in the U.S. have shown persistent neurodevelopmental effects of prenatal organophosphate (OP) insecticide exposure, the neurodevelopmental pathways and timing of exposure leading to adverse outcomes is not clear. Thus, regulatory agencies have been reluctant to change risk assessment standards or provide specific recommendations about exposures to pregnant women. The proposed longitudinal birth cohort study in Thailand will: 1) build capacity of Thai researchers to develop innovative and competitive studies of pesticide related neurotoxicity 2) measure metabolites of OP and pyrethroid (PYR) insecticides during each trimester of pregnancy, and 3) evaluate the impact of prenatal OP (and PYR as exploratory analysis) insecticide exposure on neurodevelopmental trajectories from birth to age 3. In collaboration with Chulalongkorn University (CU) in central Thailand and Chiang Mai University (CMU) in northern Thailand, 300 pregnant women will be recruited. Eight serial urine samples, 4 serial blood samples, and umbilical cord blood will be collected during pregnancy to document temporally-resolved insecticide exposure during each trimester. The Brazelton Neurobehavioral Assessment Scale will be administered to offspring at birth followed by tests of visual attention, regulation of emotion, memory and inhibitory control to determine how the “cascade” of neurologic development ultimately affects overall cognitive function (e.g., Bayley-III). Capacity building activities include development of core analytic laboratory facilities at CMU and neurodevelopmental facilities at CU along with courses to promote grant writing and international scientific publications.