Xenbase: a Xenopus Model Organism Database

Xenbase：非洲爪蟾模式生物数据库

• 批准号: 8678968
• 负责人: PETER D VIZE
• 金额: $ 85.96万
• 依托单位: CINCINNATI CHILDRENS HOSP MED CTR
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2015-12-10
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8678968
• 关键词: African; Amino Acid Sequence; Animal Model; Biochemical; Bioinformatics; Biological; Biological Models; Cells; Communities; Complementary DNA; Data; Data Analyses; Data Sources; Databases; Development; Developmental Cell Biology; Disease; Education; Embryo; Embryology; Expressed Sequence Tags; Funding; Future; Gene Expression; Generations; Genes; Genome; Genomics; Goals; Health; Human; Human Genome; Image; Instruction; Length; Link; Literature; Manuals; Maps; Messenger RNA; Microarray Analysis; Microinjections; Mining; National Institute of Child Health and Human Development; Orthologous Gene; Pathway interactions; Peptide Sequence Determination; Phenotype; Play; Process; Proteins; Publications; RNA Splicing; Rana; Regulator Genes; Research; Research Personnel; Resources; Role; Services; System; TACSTD1 gene; Testing; Transgenic Organisms; Variant; Vertebrates; Xenopus; Xenopus laevis; comparative; data mining; egg; empowered; functional genomics; gain of function; gene function; high throughput screening; improved; in vivo; insight; member; model organisms databases; mutant; success; tool

Animal models provide powerful experimental tools to test gene function and interrogate biological pathways. Comparative functional genomics between humans and model organisms has led to a wealth of discoveries, and it is clear that this will continue to play an important role in unlocking the potential of the genome for human health. In this post genomic era highly annotated and easily accessible data are increasingly essential for researchers to integrate vast amounts of sequence, expression and functional data into a meaningful biological synthesis. The goal of this proposal is to maintain and enhance Xenbase; the Xenopus model organism database. The African frogs Xenopus has long been one of the premiere model organisms used by cell and developmental biologists, providing numerous fundamental insights. The large and abundant Xenopus embryos enable sophisticated embryology, easy manipulation of gene expression by microinjection and biochemical analyses. Together with recent advances in genome and EST sequencing, the generation of full-length cDNA sets, microarray analysis, functional genomics screens, gene knockdown using anitsense morpholino oligos, the development of rapid transgenics and the establishment of Xenbase has made Xenopus a powerful system for functional genomics. Xenbase is a comprehensive database that provides a portal to inter-related Xenopus data including genomic, mRNA and protein sequence, gene expression, function and publications, which is all highly integrated with NCBI and other model organism databases. The goal of this proposal is to maintain and expand Xenbase. Aim 1) Maintain and further curate the data in Xenbase by implementing Testpresso data mining and manual curation. Aim 2) Enhance Xenbase functionality by introducing morpholino and mutant phenotypes. Aim 3) Support new content on Xenbase gene pages including mRNA splicing microarrays, and a Xenopus WIKI. Aim 4) Continue and expand collaborative research and service efforts by further integrating Xenbase data with NCBI and other model organism data bases. This proposal will empower Xenopus research and provide the broader scientific community a means of accessing a wealth of Xenopus data.

动物模型为测试基因功能和询问生物学提供了强大的实验工具 小路。人类和模式生物之间的比较功能基因组学导致了丰富的 显然，这将继续发挥重要作用，释放 人类健康的基因组。在这个后基因组时代，高度注释和易于访问的数据 对于研究人员来说，整合大量的序列、表达和功能数据越来越重要 变成一种有意义的生物合成。该提案的目标是维护和增强Xenbase； 非洲爪哇模式生物数据库。非洲青蛙长期以来一直是首屈一指的模特之一 供细胞和发育生物学家使用的生物体，提供了许多基本的见解。大的 和丰富的非洲爪哇胚胎使复杂的胚胎学，轻松操纵基因表达 通过微量注射和生化分析。以及基因组和EST的最新进展 测序、全长cdna集合的生成、微阵列分析、功能基因组学筛选、基因 利用反义吗啉寡核苷酸进行基因敲除，快速转基因技术的发展和建立 Xenbase的研究使Xenopus成为一个强大的功能基因组学系统。Xenbase是一款全面的 数据库，提供非洲爪哇相关数据的门户，包括基因组、mRNA和蛋白质 序列、基因表达、功能和出版物，所有这些都与NCBI和其他 生物模型数据库。这项提议的目标是维护和扩大Xenbase。目标1)维护 并通过实施Testpresso数据挖掘和手动管理，进一步对Xenbase中的数据进行管理。目标 2)通过引入吗啉基和突变表型来增强Xenbase的功能。目标3)支持新的 Xenbase基因页面上的内容包括mRNA剪接微阵列和一个Xenopus Wiki。目标4) 通过进一步将Xenbase数据与 NCBI和其他模式生物数据库。这项提案将增强非洲爪哇的研究能力，并提供 更广泛的科学界提供了获取丰富的非洲爪哇数据的手段。