Xenbase: a Xenopus Model Organism Database

Xenbase：非洲爪蟾模式生物数据库

• 批准号: 8678968
• 负责人: PETER D VIZE
• 金额: $ 85.96万
• 依托单位: CINCINNATI CHILDRENS HOSP MED CTR
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2015-12-10
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8678968
• 关键词: African; Amino Acid Sequence; Animal Model; Biochemical; Bioinformatics; Biological; Biological Models; Cells; Communities; Complementary DNA; Data; Data Analyses; Data Sources; Databases; Development; Developmental Cell Biology; Disease; Education; Embryo; Embryology; Expressed Sequence Tags; Funding; Future; Gene Expression; Generations; Genes; Genome; Genomics; Goals; Health; Human; Human Genome; Image; Instruction; Length; Link; Literature; Manuals; Maps; Messenger RNA; Microarray Analysis; Microinjections; Mining; National Institute of Child Health and Human Development; Orthologous Gene; Pathway interactions; Peptide Sequence Determination; Phenotype; Play; Process; Proteins; Publications; RNA Splicing; Rana; Regulator Genes; Research; Research Personnel; Resources; Role; Services; System; TACSTD1 gene; Testing; Transgenic Organisms; Variant; Vertebrates; Xenopus; Xenopus laevis; comparative; data mining; egg; empowered; functional genomics; gain of function; gene function; high throughput screening; improved; in vivo; insight; member; model organisms databases; mutant; success; tool

Animal models provide powerful experimental tools to test gene function and interrogate biological pathways. Comparative functional genomics between humans and model organisms has led to a wealth of discoveries, and it is clear that this will continue to play an important role in unlocking the potential of the genome for human health. In this post genomic era highly annotated and easily accessible data are increasingly essential for researchers to integrate vast amounts of sequence, expression and functional data into a meaningful biological synthesis. The goal of this proposal is to maintain and enhance Xenbase; the Xenopus model organism database. The African frogs Xenopus has long been one of the premiere model organisms used by cell and developmental biologists, providing numerous fundamental insights. The large and abundant Xenopus embryos enable sophisticated embryology, easy manipulation of gene expression by microinjection and biochemical analyses. Together with recent advances in genome and EST sequencing, the generation of full-length cDNA sets, microarray analysis, functional genomics screens, gene knockdown using anitsense morpholino oligos, the development of rapid transgenics and the establishment of Xenbase has made Xenopus a powerful system for functional genomics. Xenbase is a comprehensive database that provides a portal to inter-related Xenopus data including genomic, mRNA and protein sequence, gene expression, function and publications, which is all highly integrated with NCBI and other model organism databases. The goal of this proposal is to maintain and expand Xenbase. Aim 1) Maintain and further curate the data in Xenbase by implementing Testpresso data mining and manual curation. Aim 2) Enhance Xenbase functionality by introducing morpholino and mutant phenotypes. Aim 3) Support new content on Xenbase gene pages including mRNA splicing microarrays, and a Xenopus WIKI. Aim 4) Continue and expand collaborative research and service efforts by further integrating Xenbase data with NCBI and other model organism data bases. This proposal will empower Xenopus research and provide the broader scientific community a means of accessing a wealth of Xenopus data.

动物模型提供了强大的实验工具来测试基因功能和询问生物学 途径。人类和模式生物之间的比较功能基因组学已经产生了大量的 的发现，很明显，这将继续在释放该技术的潜力方面发挥重要作用 人类健康的基因组。在这个后基因组时代，高度注释且易于访问的数据 对于研究人员来说，整合大量序列、表达和功能数据变得越来越重要 转化为有意义的生物合成。该提案的目标是维护和增强Xenbase；这 非洲爪蟾模式生物数据库。非洲青蛙非洲爪蟾长期以来一直是首要模型之一 细胞和发育生物学家使用的生物体，提供了许多基本见解。大的 丰富的非洲爪蟾胚胎可实现复杂的胚胎学，轻松操纵基因表达 通过显微注射和生化分析。结合基因组和 EST 的最新进展 测序、全长 cDNA 组的生成、微阵列分析、功能基因组学筛选、基因 使用反义吗啉寡核苷酸进行敲低、快速转基因技术的发展和建立 Xenbase 使 Xenopus 成为功能基因组学的强大系统。 Xenbase 是一个综合性的 数据库，提供相互关联的非洲爪蟾数据（包括基因组、mRNA 和蛋白质）的门户 序列、基因表达、功能和出版物，均与 NCBI 等高度集成 模式生物数据库。该提案的目标是维护和扩展 Xenbase。目标 1) 维护 并通过实施 Testpresso 数据挖掘和手动管理来进一步管理 Xenbase 中的数据。目的 2) 通过引入吗啉代和突变表型增强 Xenbase 功能。目标 3) 支持新的 Xenbase 基因页面上的内容，包括 mRNA 剪接微阵列和 Xenopus WIKI。目标 4) 通过进一步将 Xenbase 数据与 NCBI 和其他模式生物数据库。该提案将增强非洲爪蟾的研究能力并提供 更广泛的科学界是获取丰富的非洲爪蟾数据的一种手段。