Refinement of an autodissemination station for control of mosquito vectors

改进用于控制蚊媒的自动传播站

• 批准号: 8646663
• 负责人: Michael Gilbert Banfield
• 金额: $ 72.25万
• 依托单位: BANFIELDBIO INC.
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-01 至 2015-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8646663
• 关键词: Adoption; Adult; Aedes; Animals; Area; Biodegradation; Biological Assay; Breeding; Culicidae; Dengue; Devices; Disease; Disease Outbreaks; Dose; Drug Formulations; Economics; Effectiveness; Environment; Environmental Risk Factor; Exclusion; Female; Fertility; Government Agencies; Habitats; Health; Hot Spot; Human; Insecticides; Juvenile Hormones; Laboratories; Laboratory Study; Lead; Longevity; Maintenance; Marketing; Methods; Military Personnel; Mosquito Control; Oviposition; Patient currently pregnant; Phase; Population; Population Control; Production; Public Health; Recommendation; Research; Resistance; Risk; Sales; Sentinel; Series; Site; Sum; Technology; Testing; Time; Universities; Vaccines; Visit; West Nile virus; chikungunya; cost; cost effective; design; field study; interest; large scale production; minimal risk; novel; prevent; prototype; public health relevance; tool; toxicant; urban area; user-friendly; vector; vector control; vector mosquito; water quality

DESCRIPTION (provided by applicant): The urban container-breeding mosquitoes Aedes albopictus and Aedes aegypti are vectors for deadly and debilitating diseases such as dengue and Chikungunya. There is no known vaccine or treatment for either disease; mosquito control is the only effective means of preventing and managing outbreaks. But container species are difficult to manage using conventional vector control technologies due to their numerous small, cryptic habitats. Autodissemination - wherein female mosquitoes contaminated with the low-risk insect growth regulator pyriproxyfen transport lethal concentrations to additional larval habitats offers the promise of effective, economic and environmentally friendly control for these species. In Phase I, collaborative efforts between SpringStar and the Rutgers University, an autodissemination station has been developed and refined for Ae. albopictus. The station was highly effective in cage and room studies at attracting and contaminating oviposition-seeking females, and releasing lethal concentrations of pyriproxyfen into subsequent containers visited. The Phase I station prototype has high attraction, exclusion from oviposition, unidirectional design, specialized formulations, extended activity, and is maintenance-free, biodegradable, user-friendly and low-risk. Phase II testing will extend the concept to Ae. aegypti, assess the effect of field variables on station efficacy, assess field deployment on localized mosquito populations in cryptic habitats, and modify non-essential portions of the station to yield a low-cost product. Prototype stations will be tested in field and semi-field conditions to determine how far the pyriproxyfen can be disseminated, how well it reaches cryptic habitats, and the impact of competing site abundance, size, and water quality. Cage and room tests will determine effectiveness of the current design against Ae. aegypti, evaluate alternate oviposition attractants against both Ae. albopictus and Ae. aegypti, and assess the effect of our formulations on adult longevity, fecundity, and fertility. Crucial field trials will center on replicated focal points ofhigh mosquito activity ('hot spots'). Field- stored units will be tested over time for structural stabilty, efficacy duration, and biodegradation. The prototype will be modified as necessary to achieve optimal efficacy, cost, and versatility. The final design resulting at the end of this phase will b suitable for large-scale production.

描述（由申请人提供）：城市集装箱繁殖的蚊子艾德斯白化病和埃及埃及是致命和使人衰弱的疾病（例如登革热和奇肯古尼亚）的媒介。没有已知的疫苗或任何两种疾病的治疗方法；蚊子控制是预防和管理暴发的唯一有效手段。但是，由于其众多的小型隐性栖息地，使用常规矢量控制技术很难使用集装箱物种来管理。自动解剖 - 其中的女性蚊子被低风险的昆虫生长调节剂羟基苯芬传输致死浓度到其他幼虫栖息地提供了对这些物种有效，经济和环境友好控制的希望。在第一阶段，Springstar与Rutgers大学之间的合作努力，为AE开发和完善了自动启动站。白化病。该站在笼子和房间研究中非常有效，吸引和污染了寻求产卵的女性，并释放出致命的羟基苯芬浓度为随后参观的容器。 I期电台原型具有很高的吸引力，排除在产卵，单向设计，专业配方，扩展活动，并且无维护，可生物降解，用户友好和低风险。第二阶段的测试将将概念扩展到AE。埃及，评估场变量对站点功效的影响，评估对隐性栖息地中局部蚊子种群的现场部署，并修改站点的非必需部分以产生低成本产品。原型站将在现场和半场条件下进行测试，以确定如何 乙芬太尼可以传播，它到达神秘栖息地的水平以及竞争地点丰度，大小和水质的影响。笼子和房间测试将确定当前设计对AE的有效性。埃及，评估替代的产卵吸引剂 反对两个AE。白化病和AE。埃及，并评估我们配方对成人寿命，生育和生育的影响。关键的现场试验将集中在复制的蚊子活动（“热点”）的焦点上。现场存储的单元将随着时间的推移测试，以实现结构稳定，有效性持续时间和生物降解。原型将根据需要进行修改，以实现最佳功效，成本和多功能性。在此阶段结束时产生的最终设计将B适合大规模生产。