Refinement of an autodissemination station for control of mosquito vectors

描述（由申请人提供）：城市容器繁殖蚊子白纹伊蚊和埃及伊蚊是登革热和基孔肯雅热等致命和使人衰弱的疾病的媒介。这两种疾病都没有已知的疫苗或治疗方法;蚊子控制是预防和管理疫情的唯一有效手段。但是，由于集装箱物种的栖息地众多且小而隐蔽，因此难以使用传统的病媒控制技术进行管理。自体传播-受低风险昆虫生长调节剂吡丙醚污染的雌蚊将致死浓度传播到其他幼虫栖息地，这为有效、经济和环境友好地控制这些物种提供了希望。在第一阶段，SpringStar和罗格斯大学之间的合作努力，为Ae开发和改进了一个自动传播站。白纹伊蚊该站是非常有效的笼子和房间的研究，在吸引和污染产卵寻求女性，并释放致死浓度的吡丙醚到随后访问的容器。第一阶段站原型具有高吸引力，排除产卵，单向设计，专门配方，扩展活动，免维护，可生物降解，用户友好和低风险。第二阶段测试将把概念扩展到Ae。埃及，评估实地变量对监测站效率的影响，评估对隐蔽生境中局部蚊子种群的实地部署，并修改监测站的非必要部分，以产生低成本产品。将在野外和半野外条件下对原型台站进行测试，以确定 吡丙醚能传播多远，它到达隐蔽生境的程度如何，以及竞争地点的丰度、大小和水质的影响。笼子和房间测试将确定当前设计对Ae的有效性。埃及伊蚊，评价交替产卵引诱剂 两个人都是Ae。albopictus和Ae.埃及伊蚊，并评估我们的制剂对成虫寿命、繁殖力和生育力的影响。关键的实地试验将集中在蚊子活动频繁的复制中心（“热点”）。现场储存的单位将进行测试，随着时间的推移，结构稳定性，有效期，和生物降解.原型将根据需要进行修改，以实现最佳功效，成本和多功能性。在此阶段结束时产生的最终设计将B适合大规模生产。

DESCRIPTION (provided by applicant): The urban container-breeding mosquitoes Aedes albopictus and Aedes aegypti are vectors for deadly and debilitating diseases such as dengue and Chikungunya. There is no known vaccine or treatment for either disease; mosquito control is the only effective means of preventing and managing outbreaks. But container species are difficult to manage using conventional vector control technologies due to their numerous small, cryptic habitats. Autodissemination - wherein female mosquitoes contaminated with the low-risk insect growth regulator pyriproxyfen transport lethal concentrations to additional larval habitats offers the promise of effective, economic and environmentally friendly control for these species. In Phase I, collaborative efforts between SpringStar and the Rutgers University, an autodissemination station has been developed and refined for Ae. albopictus. The station was highly effective in cage and room studies at attracting and contaminating oviposition-seeking females, and releasing lethal concentrations of pyriproxyfen into subsequent containers visited. The Phase I station prototype has high attraction, exclusion from oviposition, unidirectional design, specialized formulations, extended activity, and is maintenance-free, biodegradable, user-friendly and low-risk. Phase II testing will extend the concept to Ae. aegypti, assess the effect of field variables on station efficacy, assess field deployment on localized mosquito populations in cryptic habitats, and modify non-essential portions of the station to yield a low-cost product. Prototype stations will be tested in field and semi-field conditions to determine how far the pyriproxyfen can be disseminated, how well it reaches cryptic habitats, and the impact of competing site abundance, size, and water quality. Cage and room tests will determine effectiveness of the current design against Ae. aegypti, evaluate alternate oviposition attractants against both Ae. albopictus and Ae. aegypti, and assess the effect of our formulations on adult longevity, fecundity, and fertility. Crucial field trials will center on replicated focal points ofhigh mosquito activity ('hot spots'). Field- stored units will be tested over time for structural stabilty, efficacy duration, and biodegradation. The prototype will be modified as necessary to achieve optimal efficacy, cost, and versatility. The final design resulting at the end of this phase will b suitable for large-scale production.