Refinement of an Autocidal Gravid Ovitrap for mass-manufacturing

改进用于大规模生产的自杀妊娠产卵器

• 批准号: 9276569
• 负责人: Michael Gilbert Banfield
• 金额: $ 61.85万
• 依托单位: BANFIELDBIO INC.
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-01 至 2019-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9276569
• 关键词: Address; Adhesives; Adult; Aedes; Affect; Arboviruses; Area; Biological Assay; Breeding; Centers for Disease Control and Prevention (U.S.); Chemicals; Communicable Diseases; Computer-Aided Design; Country; Culicidae; Data; Dengue; Development; Devices; Diagnostic; Diagnostic Procedure; Disease; Disease Outbreaks; Dose; Ecosystem; Effectiveness; Elements; Exclusion; Female; Florida; Formulation; Frequencies; Funding; Geography; Goals; Gravid; Gravidity; Home environment; Individual; Industry; Infection; Infusion procedures; Injection of therapeutic agent; Insecticides; Lead; Life; Mission; Modification; Molds; Monitor; Mosquito Control; Neighborhoods; Performance; Persons; Pesticides; Phase; Population; Prevalence; Process; Production; Public Health; Puerto Rico; Quality of life; Quick Test for Liver Function; Regulation; Research; Risk; Seasons; Ships; Site; Source; Technology; Testing; Tropical Disease; United States National Institutes of Health; Vector-transmitted infectious disease; Virus Diseases; Water; Work; Yellow Fever; Zoonoses; base; chikungunya; commercialization; cost; cost effective; design; design and construction; digital; disorder control; efficacy testing; egg; field study; improved; killings; pesticide resistance; prevent; prototype; public health relevance; success; three-dimensional modeling; tool; transmission process; urban area; urban setting; user-friendly; vector; vector control; vector mosquito

 DESCRIPTION (provided by applicant): Lethal ovitraps are a modification of a common mosquito population monitoring technology: adult females are enticed to a water container to lay their eggs and are exposed to a lethal dose of pesticide. The Autocidal Gravid Ovitrap (AGO) improves this technology in several ways. It has been modified to make it more attractive to gravid Aedes aegypti. The killing agent is an adhesive, thus extending the useful field life while negating the need for insecticides. This project aims to modify the current AGO design to make it suitable for mass production. The goal is to produce an efficacious, cost-effective, and durable AGO. Long-term objectives include optimizing use of the AGO for control of arbovirus-vectoring mosquitoes to prevent outbreaks of dengue and other diseases, and extending the technology for use with other container-breeding species of mosquitoes. The final result is expected to be a commercially viable product for professional and home use. Phase I efforts culminated in a prototype injection mold for the AGO, with individual elements of the design validated both digitally and in competitive cage bioassays. Multiple synthetic attractants were evaluated, with several performing at least as well as the standard hay infusion. The Phase I AGO is suitable for mass production, cost-effective, user-friendly, and has high attraction to Ae. aegypti. Phase II will result in a product that can be mass-manufactured with demonstrated efficacy against container- breeding mosquitoes. The injection-molded AGO will be evaluated in large room and field tests for effectiveness and durability. The attractant formulation will be refined in arena ad field tests to be at least as attractive as hay infusion, and easily packaged and shipped. Large-scale urban field testing will be expanded to test the AGO's efficacy in suppressing both Ae. aegypti and Ae. albopictus. A CDC-developed diagnostic "quick test" will be used to determine disease frequency in trapped mosquitoes, to assess if large-scale deployment of the AGO reduces the mosquito population below the dengue transmission threshold. Container-breeding mosquitoes such as Ae. aegypti and Ae. albopictus are day-biting nuisance mosquitoes. They are also vectors of serious viral diseases, including dengue fever, yellow fever, and chikungunya. Currently >2.5 billion people in tropical countries are at risk of infection with dengue fever. The mission of the CDC-NCEZID is to detect, prevent, and control zoonotic infectious diseases. Dengue fever and chikungunya are not currently major threats to US residents, but these diseases are spreading as their mosquito vectors expand their ranges. The AGO will be both a monitoring tool to check the spread of these increasingly invasive species, and an effective control option for use in outbreaks by creating area-wide suppression of mosquito populations. It promises to be an important tool in the long term battle to control nuisance mosquitoes, improve quality of life for people in affected areas, and prevent vector-borne diseases.

 描述（由申请人提供）：致命产卵器是对常见蚊子种群监测技术的改进：成年雌性被引诱到水容器中产卵，并暴露于致命剂量的杀虫剂中。自杀妊娠产卵器 (AGO) 在几个方面改进了这项技术。它经过修改，使其对怀孕的埃及伊蚊更具吸引力。杀虫剂是一种粘合剂，因此可以延长田间使用寿命，同时无需使用杀虫剂。该项目旨在修改当前的AGO设计，使其适合批量生产。目标是生产一种有效的、具有成本效益的、耐用的 前。长期目标包括优化 AGO 的使用，以控制传播虫媒病毒的蚊子，以防止登革热和其他疾病的爆发，并将该技术推广到其他容器繁殖的蚊子种类。最终结果预计将是一款适合专业和家庭使用的商业上可行的产品。第一阶段的努力最终形成了 AGO 原型注塑模具，设计的各个元素都经过了数字化和竞争性笼式生物测定的验证。对多种合成引诱剂进行了评估，其中几种的效果至少与标准干草浸剂一样好。一期AGO适合量产，性价比高，用户友好，对Ae有较高的吸引力。埃及伊蚊。第二阶段将生产出一种可以大规模生产的产品，并已证明对容器繁殖的蚊子有效。注塑成型的 AGO 将在大房间和现场测试中进行有效性和耐用性评估。引诱剂配方将在竞技场和现场测试中进行改进，使其至少与干草浸液一样有吸引力，并且易于包装和运输。将扩大大规模城市现场测试，以测试 AGO 抑制伊蚊的功效。埃及伊蚊和伊蚊。白纹伊蚊。 CDC 开发的诊断“快速测试”将用于确定被困蚊子的疾病频率，以评估大规模部署 AGO 是否将蚊子数量减少到登革热传播阈值以下。容器繁殖的蚊子，如伊蚊。埃及伊蚊和伊蚊。白纹伊蚊是日间叮人的讨厌蚊子。它们也是严重病毒性疾病的传播媒介，包括登革热、黄热病和基孔肯雅热。目前，热带国家超过 25 亿人面临感染登革热的风险。 CDC-NCEZID 的使命是检测、预防和控制人畜共患传染病。登革热和基孔肯雅热目前并不是美国居民的主要威胁，但随着蚊媒传播范围的扩大，这些疾病正在蔓延。 AGO 既是检查这些日益入侵的物种传播的监测工具，也是通过在大范围内抑制蚊子种群来控制疫情爆发的有效控制选择。它有望成为控制蚊子、改善受影响地区人民的生活质量和预防媒介传播疾病的长期斗争的重要工具。