Investigating tumour biology using regulated RNAi in cells and mice

使用最近发现的称为RNA干扰（RNAi）的过程抑制基因表达可用作分析细胞和人类疾病的基因工程动物模型中特定基因的实验工具。我建议使用RNAi在小鼠癌症模型中模拟人类癌症基因突变，并旨在发现新的肿瘤抑制基因。另一个目的是通过使用RNAi抑制已建立的小鼠肿瘤中的特定基因来验证癌症中的潜在药物靶点。

Inhibiting gene expression using the recently discovered process known as RNA interference (RNAi) can be used as an experimental tool to analyse specific genes, in cells and genetically engineered animal models of human disease. I propose to use RNAi to mimic human cancer gene mutations in mouse cancer models, and aim to discover novel tumour suppressor genes. A further aim is to validate potential drug targets in cancer by using RNAi to inhibit specific genes in established mouse tumours.