Lactic Acid Bacteria that Detect & Inhibit Enterococci in the Mammalian GI Tract
检测的乳酸菌
基本信息
- 批准号:8747170
- 负责人:
- 金额:$ 35.3万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2014
- 资助国家:美国
- 起止时间:2014-08-01 至 2018-04-30
- 项目状态:已结题
- 来源:
- 关键词:AddressAnimal ExperimentsAnimal ModelAnimalsAntibiotic ResistanceAntibiotic TherapyAntibioticsBacteriaBacteriophagesBenchmarkingBile fluidBioavailableBiological MetamorphosisBlood CirculationCell WallCellsChloride IonChloridesCleaved cellCloningCommunicable DiseasesCommunicationCytolysisDetectionDevelopmentDiseaseEngineeringEnterococcusEnterococcus faecalisEnterococcus faeciumEnvironmentEnzymesFeedbackFutureGastrointestinal tract structureGene ExpressionGenesGeneticGenetic EngineeringGoalsHealth Care CostsHealthcare SystemsHeartHospitalsHumanIncidenceIndividualInfectionInterventionIntestinesLactobacillusLactococcusLactococcus lactisLeadLength of StayLiverMammalsMembraneMembrane ProteinsMethodsModelingModificationMorbidity - disease rateMusNosocomial InfectionsOperonOrganismPatientsPeptide HydrolasesPeptidesPeptidoglycanPheromonePlasmidsProbabilityProbioticsProductionProtein EngineeringProteinsReporter GenesReportingResistanceResistance developmentResortSeveritiesSignal TransductionSkinSpleenStomachStructureSurfaceSystemTechnologyTestingTherapeuticVacuumVancomycinVancomycin resistant enterococcusVirulenceWorkantimicrobialantimicrobial peptidebile saltsbiophysical propertiescostdirected evolutionendolysinenterocinfightinggastrointestinalimprovedin vivoinsightkillingslactic acid bacteriamathematical modelmicroorganismmortalitymulti-scale modelingpathogenpeptide pheromone cCF10promoterprotein expressionpublic health relevanceresearch studyresponsescreeningspatiotemporaltherapeutic development
项目摘要
DESCRIPTION (provided by applicant): Enterococci are bacteria that grow naturally inside human intestines. Over the last few decades, these pathogens have developed resistance to our most potent antibiotics, including vancomycin, an antibiotic of last resort for infectious disease. Vancomycin-resistant Enterococcus (VRE) does not cause illness to healthy individuals. But it does cause severe illness to hospital patients, especially the ones who have undergone antibiotic therapy. Antibiotic therapy eliminates a lot of the "good" bacteria in the gut
of humans. VRE grab and fill this vacuum, spreading quickly from the intestines to the bloodstream and from there to the spleen, the liver, the heart. There they grow and cause disease that can no longer be easily treated with antibiotics. We genetically engineer lactic acid
bacteria (LAB) to specifically target enterococcus inside gastrointestinal (GI) tracts. We propose to administer these cellbots to mice and examine if enterococcus vanishes from mouse GI tracts. We pursue three specific aims: First we engineer "smart" LAB that can detect enterococci. Upon detection, engineered LAB produce and release proteins. In the second aim, we engineer these proteins to be lethal to enterococci. In the third aim, we will examine the efficacy of our modified LAB in mice. We will infect mice with enterococcus and then administer LAB to them. The main hypothesis is that engineered LAB kill enterococci and stop their colonization inside animal GI tracts. If successful, these experiments can lead to the development of a promising therapeutic strategy against enterococcal infections.
描述(由申请人提供):肠球菌是在人体肠道内自然生长的细菌。在过去的几十年里,这些病原体已经对我们最有效的抗生素产生了耐药性,包括万古霉素,一种治疗传染病的最后手段。万古霉素耐药肠球菌(VRE)不会引起健康个体的疾病。但它确实会给住院病人带来严重的疾病,尤其是那些接受过抗生素治疗的病人。抗生素治疗会消除肠道中的许多“好”细菌
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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GARY M DUNNY其他文献
GARY M DUNNY的其他文献
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{{ truncateString('GARY M DUNNY', 18)}}的其他基金
Functional genomics analysis of colonization and persistence of Enterococcus faecalis in the gastrointestinal tract.
粪肠球菌在胃肠道中定植和持续存在的功能基因组学分析。
- 批准号:
9215645 - 财政年份:2016
- 资助金额:
$ 35.3万 - 项目类别:
Pathway for functional characterization of hypothetical genes and non-coding RNAs of Enterococcus faecalis
粪肠球菌假设基因和非编码 RNA 功能表征途径
- 批准号:
8986937 - 财政年份:2015
- 资助金额:
$ 35.3万 - 项目类别:
Lactic Acid Bacteria that Detect & Inhibit Enterococci in the Mammalian GI Tract
检测的乳酸菌
- 批准号:
9060971 - 财政年份:2014
- 资助金额:
$ 35.3万 - 项目类别:
Lactic Acid Bacteria that Detect & Inhibit Enterococci in the Mammalian GI Tract
检测的乳酸菌
- 批准号:
9272922 - 财政年份:2014
- 资助金额:
$ 35.3万 - 项目类别:
Invasion and Exclusion by Enterococcus faecalis in the Manduca gut community
粪肠球菌对天蛾肠道群落的入侵与排除
- 批准号:
8412944 - 财政年份:2012
- 资助金额:
$ 35.3万 - 项目类别:
4th ASM Conference on Cell-Cell Communication in Bacteria
第四届 ASM 细菌细胞间通讯会议
- 批准号:
8205337 - 财政年份:2011
- 资助金额:
$ 35.3万 - 项目类别:
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