Oxarane-Acrylate System to Double the Clinical Service Life of Restorative Resins

氧杂环丙烷-丙烯酸酯系统可将修复树脂的临床使用寿命延长一倍

• 批准号: 8729445
• 负责人: H. RALPH RAWLS
• 金额: $ 44.25万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8729445
• 关键词: Accounting; Acrylates; Address; Animals; Anti-Bacterial Agents; Award; Bacteria; Biochemical; Biocompatible Materials; Biomedical Engineering; Bisphenol A-Glycidyl Methacrylate; Calcium; Characteristics; Chemical Engineering; Clinical; Clinical Research; Clinical Services; Color; Composite Dental Resin; Delayed Hypersensitivity; Dental; Dental Enamel; Dental Pulp; Dental caries; Dentin; Dentistry; Doctor of Philosophy; Environment; Esters; Ethylene Oxide; Event; Exposure to; Fatigue; Filler; Fluorine; Goals; Hydrolysis; Hydrophobicity; Hydroquinones; Hypersensitivity; In Situ; In Vitro; Incidence; Ions; Life; Longevity; Mastication; Mechanics; Methacrylates; Microbial Biofilms; Minerals; Modeling; Nature; One-Step dentin bonding system; Oral; Oral cavity; Oral health; Oral mucous membrane structure; Oxygen; Performance; Plant Resins; Polymer Chemistry; Polymers; Predisposition; Property; Recurrence; Relaxation; Residual state; Resistance; Science; Scientist; Side; Silver; Solid; Stress; Structure; Surface; System; Testing; Urethane; Viscosity; Water; Work; analog; antimicrobial; bacterial resistance; base; biomaterial compatibility; bisphenol A; cost; crosslink; dental resin; design; dioxirane; esterase; hydroquinone; in vivo; innovation; inorganic phosphate; irritation; meetings; monomer; multidisciplinary; nanoparticle; novel; physical property; polymerization; public health relevance; response; restoration; restorative dentistry; restorative resins; triethylene glycol dimethacrylate; uptake

DESCRIPTION (provided by applicant): In order to develop a novel restorative system with at least twice the lifetime of Bis-GMA/TEGDMA- based composites, their incomplete cure and susceptibility to hydrolysis and esterase degradation must be overcome. To address these problems, we will develop a novel superhydrophobic, degradation-resistant, dental restorative based on an Oxirane/Acrylate interpenetrating network System (OASys, pronounced Oasis). These novel monomers based on fluoridated urethanes with either dioxirane or diacrylate functionality can be highly converted to form a hydrophobic, degradation-resistant, tough and resilient interpenetrating polymer network (IPN) that is inherently highly crosslinked. By their nature, these characteristics impart low residual stresses, high resistance to hydrolytic and enzymatic degradation, and biocompatibility. We will also develop a novel one-step (primer-less), "smart," antimicrobial bonding resin with in situ-generated, colorless and color stable, silver nanoparticles (AgNPs). The bonding resin will contain a phosphate group plus both oxirane and acrylate functionalities. The oxirane and acrylate functionalities bond to the corresponding functionalities in the IPN resin matrix for potentially a much stronger bond than the conventional methacrylate system. The phosphate group will allow the bonding resin to wet etched mineral surfaces as well as bond directly to calcium in Ca-phosphate mineral structures. In the event of marginal gap formation, the "smart" in situ-generated AgNPs will release Ag+ ions and create an antibacterial environment, thereby further reducing the incidence of recurrent caries. Five specific aims are proposed: 1. To determine the effect of using oxiranes, increased hydrophobicity, and IPNs on resin mechanical properties, physical properties and in vitro biocompatibility. The more promising compositions will be combined with reinforcing filler and used for Aim 2. 2. To determine the effect of using a 4- Phospho-NPG GA oxirane (4POA)-based bonding system and in situ-generated silver nanoparticles (AgNP) on bonding resin mechanical properties, physical properties, and in vitro biocompatibility and antibacterial activit, as well as on bond strength to oxirane/acrylate interpenetrating network composites. The two best- performing composites will be chosen for subsequent aims. 3. To determine the effect of using oxiranes, increased hydrophobicity, and IPNs on resin resistance to the oral biochemical environment. The two best- performing groups chosen in Aim 2 will be fatigue- and wear-tested after exposure to acidic, basic and esterase-containing environments for 90 days. 4. To determine the effect of using oxiranes, increased hydrophobicity, and IPNs on resin resistance to bacterial degradation. The two best-performing groups from Aim 2 will be tested in an artificial mouth bacterial biofilm model. 5. To determine the in vivo biocompatibility of the OASys. The best performing OASys will be tested in three in vivo biocompatibility models: delayed-type hypersensitivity, oral mucosa irritation, and pulp and dentin response tests.

描述(由申请人提供)：为了开发一种新的修复系统，其寿命至少是Bis-GMA/TEGDMA基复合材料的两倍，必须克服它们的不完全固化以及对水解和酯酶降解的敏感性。为了解决这些问题，我们将开发一种基于环氧乙烷/丙烯酸酯互穿网络体系(OASYS，发音为OASIS)的新型超疏水、抗降解的牙科修复材料。这些基于二氧六环或二丙烯酸酯官能团的含氟氨酯的新型单体可以高度转化，形成一种疏水性、耐降解性、韧性和弹性的内在高度交联性的互穿聚合物网络(IPN)。从本质上讲，这些特性赋予了低残余应力、高耐水解性和耐酶降解性以及生物相容性。我们还将开发一种新型的一步法(无底漆)、“智能”抗菌粘合树脂，其中含有原位生成的、无色和颜色稳定的银纳米颗粒(AgNPs)。粘合树脂将包含一个磷酸基团以及环氧乙烷和丙烯酸酯官能团。环氧乙烷和丙烯酸酯官能团与IPN树脂基质中的相应官能团结合在一起，可能比传统的甲基丙烯酸酯体系具有更强的成键能力。磷酸基团将允许粘合树脂润湿蚀刻的矿物表面，并直接与钙-磷酸盐矿物结构中的钙结合。在边缘间隙形成的情况下，原位生成的智能AgNPs将释放Ag+离子并创造抗菌环境，从而进一步降低复发龋齿的发生率。提出了五个具体目标：1.确定使用环氧烷、增加疏水性和IPN对树脂的力学性能、物理性能和体外生物相容性的影响。2.研究4-磷酸-NPG-GA环氧烷(4POA)粘接体系和原位生成银纳米颗粒(AgNP)对粘接树脂力学性能、物理性能、体外生物相容性和抗菌活性的影响，以及对环氧乙烷/丙烯酸酯互穿网络复合材料的粘接强度的影响。两种性能最好的复合材料将被选为后续目标。3.确定使用环氧烷、增加疏水性和IPN对树脂对口腔生化环境的耐受性的影响。在AIM 2中选出的两个表现最好的组将在暴露于酸性、碱性和含酯酶的环境中90天后进行疲劳和磨损测试。4.确定使用环氧烷、增加疏水性和IPN对树脂抗细菌降解能力的影响。来自AIM 2的两个表现最好的小组将在一个人工 口腔细菌生物被膜模型。5.测定OASYS的体内生物相容性。表现最好的OASYS将在三种体内生物相容性模型中进行测试：迟发性超敏反应、口腔粘膜刺激以及牙髓和牙本质反应测试。