Lowering the Cost of Imaging for Retinal Microvasculature in Diabetic Patients

降低糖尿病患者视网膜微血管成像的成本

• 批准号: 8903059
• 负责人: ANN E ELSNER
• 金额: $ 22.15万
• 依托单位: AEON IMAGING, LLC
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2017-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8903059
• 关键词: Algorithmic Software; Benchmarking; Biological Markers; Blood; Blood Vessels; Blood capillaries; Blood-Retinal Barrier; Burn injury; Clinic; Clinical; Computer software; Cone; Custom; Detection; Development; Devices; Edema; Equipment; Eye; Failure; Financial compensation; Goals; Health; Healthcare; High-Cost Technology; Human; Image; Imaging Device; Indiana; Individual Differences; Institution; Insulin-Dependent Diabetes Mellitus; Lasers; Lead; Light; Maps; Measures; Methodology; Methods; Microcirculation; Ophthalmoscopes; Optics; Patients; Performance; Perfusion; Phase; Procedures; Publishing; Pump; Pupil; Research; Resolution; Retina; Retinal; Retinal Cone; Risk; Scanning; Series; Structure of retinal pigment epithelium; System; Techniques; Technology; Testing; Time; Universities; Vision; adaptive optics; capillary; contrast imaging; cost; density; design; diabetic; diabetic patient; digital; flexibility; fovea centralis; image processing; improved; in vivo imaging; light scattering; novel; older patient; optical imaging; particle; public health relevance; sensor

 DESCRIPTION (provided by applicant): Recently, we found extensive remodeling of the microvasculature of the retina, occurring in some diabetic patients much earlier than expected. This provides the potential for novel biomarkers. However, the equipment is too expensive for most clinics and small practices. These early retinal microvascular changes cannot be seen with routine clinical examination or traditional imaging devices. We propose to reduce the cost and improve ease of use of this technology to allow widespread detection of retinal microvascular changes as biomarkers. Part of the high cost of these devices is the adaptive optics (AO) subsystem, which is deformable mirrors needed to provide compensation for the aberrations of the optics of the human eye. By planning for high resolution, but less than diffraction limited resolution, a wider field of view and less extreme deformable mirror performance can be realized. The important capillary changes will be nevertheless visualized in the retina. We propose 3 Aims. In Aim 1, we will optimize a lower cost retinal imager, the Digital Light Ophthalmoscope (DLO), for high magnification and high contrast imaging that is sufficient to visualize retinal capillaries and cones outside the central fovea. Aeon will build a DLO with a mid- size for the field of view, which provides an image sufficiently large to show capillary networks in a single image, with the capillaries and cones being better visualized following image processing. This also more readily allows the montaging of AO images taken sequentially for this Phase I proposal. In Aim 2, we will develop the AO subsystem for the AO-DLO to correct aberrations of the human eye, so that the high resolution images are high contrast. We will use a commercially available, visible wavelength deformable mirror and adapt the custom AO sensor and control techniques from existing, custom software. These Hartman Shack control algorithms and software are published and have been shared with other institutions. One key feature is the ability to use a smaller region of the pupil in older subjects. In Aim 3, we wil develop an AO-DLO by integrating AO with the DLO, and map the microcirculation of the retina in 10 normal subjects. We will compare the capillary perfusion maps to corresponding maps from the AO Scanning Laser Ophthalmoscope (AO-SLO), that was used discover the retinal microvascular changes. For both AO-DLO and AO-SLO, we will produce capillary maps by collecting a series of images from the AO-DLO, aligning them, and plotting the variance of scaled images. This is possible because the moving blood particles lead to differing amounts of reflectivity over time, whereas the remaining retinal reflectivity is relatively unchanged in the wavelength range that we use. Then we will compare the test-retest of the capillary maps for each device vs. the comparison of the two devices.

 描述(申请人提供)：最近，我们发现视网膜微血管的广泛重塑，在一些糖尿病患者中发生得比预期的要早得多。这为开发新的生物标记物提供了可能性。然而，对于大多数诊所和小诊所来说，这种设备太贵了。这些早期的视网膜微血管改变不能用常规的临床检查或传统的成像设备来看到。我们建议降低成本并提高这项技术的易用性，以便广泛检测视网膜微血管变化作为生物标志物。这些设备的高成本的一部分是自适应光学(AO)子系统，它是提供人眼光学像差补偿所需的可变形反射镜。通过规划高分辨率，但小于衍射极限分辨率，可以实现更宽的视场和更少极端变形的镜面性能。尽管如此，重要的毛细血管变化仍将在视网膜上可视化。我们提出了三个目标。在目标1中，我们将优化一种成本较低的视网膜成像仪，即数字光检眼镜(DLO)，用于高倍率和高对比度成像，足以显示中央中心凹外的视网膜毛细血管和视锥。永旺将建造一个中等大小的视场DLO，它提供的图像足够大，可以在一张图像中显示毛细血管网络，经过图像处理后，毛细血管和锥体可以更好地可视化。这还可以更容易地对第一阶段提案中顺序拍摄的AO图像进行剪辑。在目标2中，我们将开发用于AO-DLO的AO子系统，以校正人眼的像差，从而使高分辨率图像具有高对比度。我们将使用市面上可见的可见波长变形反射镜，并采用现有定制软件中的定制AO传感器和控制技术。这些Hartman Shack控制算法和软件已发布，并已与其他机构共享。其中一个关键特征是，在年龄较大的受试者中，能够使用较小的瞳孔区域。在目标3中，我们将通过将AO与DLO相结合来开发一种AO-DLO，并绘制10名正常人的视网膜微循环图。我们将毛细血管灌注图与AO扫描激光眼底镜(AO-SLO)的相应图进行比较，以发现视网膜微血管的变化。对于AO-DLO和AO-SLO，我们将通过从AO-DLO收集一系列图像、对齐它们并绘制缩放图像的变化来生成毛细管图。这是可能的，因为随着时间的推移，移动的血液颗粒会导致不同数量的反射率，而剩余的视网膜反射率在我们使用的波长范围内相对不变。然后，我们将对每个设备的毛细管图的重新测试与两个设备的比较进行比较。

项目成果

Imaging the visual system: from the eye to the brain.

视觉系统成像：从眼睛到大脑。

• DOI: 10.1111/opo.12298
• 发表时间: 2016
• 期刊: Ophthalmic & physiological optics : the journal of the British College of Ophthalmic Opticians (Optometrists)
• 作者: Thompson,Benjamin;Read,ScottA;Dumoulin,SergeO;Elsner,AnnE;Porter,Jason;Roorda,Austin
• 通讯作者: Roorda,Austin