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Leptin Signaling and Alzheimer's Disease

瘦素信号传导和阿尔茨海默病

基本信息

批准号：
8919198
负责人：
Michael Paul Murphy
金额：
$ 18.25万
依托单位：
UNIVERSITY OF KENTUCKY
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-09-01 至 2016-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8919198
关键词：
Adipocytes Affect Age Age-associated memory impairment Alzheimer&apos s Disease Alzheimer&apos s disease risk Amyloid Aneurysm Animals Beryllium Biological Biological Process Brain Case Mixes Characteristics Clinical Complex Data Dementia Dependovirus Deposition Development Diabetes Mellitus Disasters Disease Electron Microscopy Employee Strikes Endothelial Cells Epidemiology Etiology Exhibits Foundations Functional disorder Future Genotype Health Hunger Hypothalamic structure Image Impaired cognition Individual Injury Knock-in Mouse Knowledge Leptin Leptin resistance Lesion Life Style Link Magnetic Resonance Imaging Mediator of activation protein Metabolic Metabolic Diseases Modeling Mus Neurodegenerative Disorders Neurofibrillary Tangles Neurologic Dysfunctions Neurons Non-Insulin-Dependent Diabetes Mellitus Obesity Pathologic Pathology Pathway interactions Patients Phenotype Play Population Predisposition Public Health Recording of previous events Resistance Risk Risk Factors Role Satiation Senile Plaques Serum Signal Transduction Societies Stroke System Testing Therapeutic Variant Vascular Dementia Weight age related aging brain amyloid peptide angiogenesis cerebrovascular cognitive function demographics diabetic disease phenotype high risk improved improved functioning innovation leptin receptor light microscopy middle age mouse leptin receptor mouse model nervous system disorder neuronal survival neuropathology novel peptide A peptide hormone prevent receptor repaired therapeutic development

项目摘要

DESCRIPTION (provided by applicant): Alzheimer's disease (AD) is the most common age-related neurodegenerative disease, currently affecting more than five million people in the U.S.A. The vast majority of AD cases are sporadic, with no clear etiology. It is well-documented that lifestyle and overall health play a role in the development of the disease. A connection exists between risk for AD and Type 2 Diabetes Mellitus (T2DM), a metabolic disease that is linked to obesity. The mechanism that underlies this epidemiological connection is unknown. Recent neuropathology studies have shown that AD cases that have a history of T2DM and obesity have substantially more cerebrovascular pathology, but have either the same or lesser amounts of neuritic plaques and neurofibrillary tangles. It may be that changes in the cerebrovasculature alter the threshold for the development of AD, or that individuals with AD and a history of obesity represent cases of mixed AD and vascular dementia (VaD). This proposal considers the possibility that these elements are connected through defective leptin signaling, since individuals with a history of obesity and diabetes are leptin resistant. Leptin is an adipocyte-derived peptide hormone that regulates satiety and hunger via signaling through the leptin receptor (LepR), and these receptors are expressed throughout the brain. To explore the connection between leptin resistance, obesity and AD, we created a unique mouse line (db/AD) that combines features of these pathophysiologic states. These mice are resistant to leptin (the LepR is inactivated), become rapidly obese and diabetic, and develop AD-related neuropathology. These mice also develop a striking phenotype of cerebrovascular pathology, and display a profound cognitive impairment. In recent years, several studies have linked leptin with cognitive function. It has even been suggested that leptin treatment may be a viable therapeutic approach towards alleviating age-related cognitive decline. This project proposes to directly test the role of leptin in cognitive function in our novel model by selectively replacing he defective LepR in the brain with a functional version via adeno-associated virus, thereby restoring leptin sensitivity. Preliminary tests indicate that we are able to replace the receptor without affecting the hypothalamus, and the animals remain hyperphagic and retain their metabolic disease phenotype. We therefore believe that this approach will allow us to separate the effects of leptin on the development of neurologic dysfunction from the many complex problems associated with obesity and systemic metabolic disease. If leptin resistance is the cause of the cognitive dysfunction in the db/AD mice, then restoring leptin sensitivity will improve function. This project, utilizing a novel and innovative mouse model, has the potential to significantly advance our understanding of the association between obesity, diabetes, and dementia. If there is a clear connection between leptin, leptin resistance and neuropathology, patients may one day benefit from preventative of therapeutic strategies targeting leptin pathways.

描述（由申请人提供）：阿尔茨海默病（AD）是最常见的年龄相关性神经退行性疾病，目前在美国影响超过500万人。绝大多数AD病例是散发性的，没有明确的病因。有充分证据表明，生活方式和整体健康状况在疾病的发展中发挥着作用。AD风险与2型糖尿病（T2 DM）之间存在联系，2型糖尿病是一种与肥胖有关的代谢疾病。这种流行病学联系背后的机制尚不清楚。最近的神经病理学研究表明，有T2 DM和肥胖病史的AD病例具有显著更多的脑血管病理学，但具有相同或更少量的神经炎斑块和神经纤维缠结。这可能是由于脑血管系统的变化改变了AD发生的阈值，或者患有AD和肥胖史的个体代表了AD和血管性痴呆（VaD）的混合病例。该建议考虑了这些元素通过缺陷的瘦素信号传导连接的可能性，因为具有肥胖和糖尿病史的个体是瘦素抵抗的。瘦素是一种脂肪细胞衍生的肽激素，通过瘦素受体（LepR）的信号传导来调节饱腹感和饥饿感，这些受体在整个大脑中表达。为了探索瘦素抵抗、肥胖和AD之间的联系，我们创建了一个独特的小鼠系（db/AD），其结合了这些病理生理状态的特征。这些小鼠对瘦素有抵抗力（LepR失活），迅速肥胖和糖尿病，并发展为AD相关的神经病理学。这些小鼠还发展出脑血管病理学的显著表型，并显示出严重的认知障碍。近年来，一些研究将瘦素与认知功能联系起来。甚至有人认为，瘦素治疗可能是一种可行的治疗方法，以减轻与年龄相关的认知能力下降。该项目提出在我们的新模型中直接测试瘦素在认知功能中的作用，通过腺相关病毒选择性地用功能性版本替换大脑中有缺陷的LepR，从而恢复瘦素敏感性。初步测试表明，我们能够在不影响下丘脑的情况下取代受体，并且动物保持摄食过多并保留其代谢疾病表型。因此，我们相信这种方法将使我们能够将瘦素对神经功能障碍发展的影响与肥胖和全身代谢疾病相关的许多复杂问题分开。如果瘦素抵抗是db/AD小鼠认知功能障碍的原因，那么恢复瘦素敏感性将改善功能。该项目利用一种新颖的创新小鼠模型，有可能显着推进我们对肥胖，糖尿病和痴呆症之间关系的理解。如果在瘦素、瘦素抵抗和神经病理学之间存在明确的联系，那么有一天患者可能会从针对瘦素途径的预防性治疗策略中受益。