Targeted Analysis Resource

有针对性的分析资源

• 批准号: 9062028
• 负责人: John D. Meeker
• 金额: $ 546.64万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-30 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9062028
• 关键词: Amino Acids; Analytical Chemistry; Aromatic Polycyclic Hydrocarbons; Benzophenones; Bile Acids; Bioinformatics; Biological; Biological Assay; Biological Markers; Biometry; Chemicals; Child; Child health care; Clinical Research; Communication; Core Facility; Development; Environment; Environmental Epidemiology; Environmental Exposure; Epidemiologic Studies; Equipment; Exposure to; Fatty Acids; Flame Retardants; Goals; Grant; Growth and Development function; Hormones; Human; Human Resources; Immune; International; Isoflavones; Knowledge; Laboratories; Leadership; Lipids; Measurement; Measures; Metabolic; Metals; Methods; Michigan; Micronutrients; Minerals; Molecular Epidemiology; Nonesterified Fatty Acids; Nutrient; Oxidative Stress; Parabens; Pesticides; Phenols; Phytoestrogens; Phytosterols; Polychlorinated Biphenyls; Procedures; Process; Psychosocial Stress; Quality Control; Reproducibility; Research Personnel; Research Support; Resources; Robin bird; Sampling; Science; Technical Expertise; Tobacco smoke; Triclosan; Universities; Validation; Vitamins; Work; bisphenol A; computerized data processing; epidemiology study; epigenomics; experience; inflammatory marker; laboratory facility; metabolomics; method development; novel; nutrition; phthalates; programs; quality assurance; response; sample collection

ABSTRACT (Targeted Analysis Resource) The primary objective of the Michigan Children’s Health Exposure Analysis Resource (M-CHEAR) Laboratory Hub is to contribute to large-scale national efforts to advance our knowledge of the impact of the environment on children’s health by offering high-quality, advanced laboratory support for a wide assortment of targeted and untargeted analyses in biological samples from external researchers conducting epidemiology and clinical studies of children’s health. The overarching goal of the M-CHEAR Targeted Analysis Resource is to provide external investigators with highly sensitive, selective, state-of-the-art targeted analysis of a broad array of biomarkers that can be effectively applied to epidemiology and clinical studies of children’s health. This will be done through leveraging core facilities at University of Michigan and the modern facilities and equipment at its partnering laboratory (NSF International), offering highly sensitive measurement methods that we have already developed in these settings and successfully applied to ongoing high-impact epidemiologic studies of children’s health, implementing and adhering to standard operating procedures and strict quality assurance/quality control processes, continually expanding our analysis capabilities, and effective internal and external communications to respond efficiently to investigator and CHEAR program needs. Specific analyses to be offered include: 1) High-throughput trace level analysis of biomarkers of exposure to a range of environmental exposures, including phthalates, bisphenol A (BPA) and other phenols (e.g., triclosan, benzophenone-3, dichlorophenols), parabens, polycyclic aromatic hydrocarbons (PAH), flame retardant chemicals, persistent and non-persistent pesticides, polychlorinated biphenyls (PCBs), perfluoroalkyl chemicals (PFCs), tobacco smoke, metals/metalloids, and others; 2) Targeted analysis of markers related to nutrition and metabolic factors including mineral nutrients, vitamins, phytoestrogens, lipids, amino acids, fatty acids, bile acids, oxysterols, phytosterols, and more; and 3) Efficient analysis of additional targeted assays of endogenous measures such as hormone levels, immune indicators, and markers of inflammation, oxidative stress, and psychosocial stress. We will work closely with the Developmental Core in the development of methods for targeted analysis of new exogenous or endogenous markers, as well as methods for measurement of these markers in novel biological matrices. The Targeted Analysis Resource will also conduct timely and high-quality data processing to identify potential errors and maximize ease of use, communicate with other CHEAR Laboratory Hubs to capitalize on one another’s capabilities and expertise, and participate in round-robin assessments to determine reproducibility of existing or new methods and to compare accuracy and precision of specific methods across CHEAR Laboratory Hubs. Finally, we will be able to offer our diverse and unique expertise in analysis, interpretation and application of the various biomarkers to epidemiology studies of children’s health to investigators and the CHEAR program.

摘要（目标分析资源） 密歇根儿童健康暴露分析资源实验室（Michigan Children's Health Exposure Analysis Resource Laboratory，M-CHEARS） 该中心旨在为大规模的国家努力做出贡献，以提高我们对环境影响的认识 通过提供高质量，先进的实验室支持， 对来自进行流行病学和临床研究的外部研究人员的生物样本进行非靶向分析 儿童健康的研究。M-CHESTIC目标分析资源的首要目标是提供 外部研究人员对广泛的 这些生物标志物可以有效地应用于儿童健康的流行病学和临床研究。这将是 通过利用密歇根大学的核心设施和其现代化的设施和设备， 合作实验室（NSF International），提供高灵敏度的测量方法，我们已经 在这些环境中开发，并成功地应用于正在进行的儿童高影响流行病学研究， 健康，执行和遵守标准操作程序和严格的质量保证/质量 控制流程，不断扩大我们的分析能力，以及有效的内部和外部 沟通，以有效地响应研究者和CHEMICAL计划的需求。具体分析如下： 提供包括：1）高通量痕量水平分析暴露于一系列环境的生物标志物 暴露，包括邻苯二甲酸酯，双酚A（BPA）和其他酚类（例如，三氯生，二苯甲酮-3， 二氯苯酚），对羟基苯甲酸酯，多环芳烃（PAH），阻燃化学品，持久性有机污染物 和非持久性农药、多氯联苯、全氟烷基化学品、烟草 吸烟、金属/类金属及其他; 2）营养和代谢相关标志物的靶向分析 包括矿物质营养素、维生素、植物雌激素、脂质、氨基酸、脂肪酸、胆汁酸 氧化固醇、植物固醇等;以及3）内源性胆固醇的额外靶向测定的有效分析。 测量如激素水平、免疫指标和炎症标志物、氧化应激， 心理压力我们将与发展核心密切合作， 新的外源性或内源性标记的靶向分析，以及测量这些标记的方法， 新生物基质中的标记物。有针对性的分析资源还将进行及时和高质量的 数据处理，以识别潜在的错误，并最大限度地提高易用性，与其他CHEMICAL 实验室中心利用彼此的能力和专业知识，并参与循环 评估以确定现有或新方法的重现性，并比较准确度和精密度 CHEMICAL实验室中心的具体方法。最后，我们将能够提供我们的多样化和独特的 在分析，解释和应用各种生物标志物的流行病学研究的专业知识， 儿童的健康调查和CHEMICAL计划。