A micro Hall chip for circulating microvesicle based cancer monitoring

用于基于循环微泡的癌症监测的微型霍尔芯片

• 批准号: 9112786
• 负责人: David Aaron Issadore
• 金额: $ 19.93万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2018-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9112786
• 关键词: Address; Antibodies; Benchmarking; Biological Assay; Biological Markers; Biological Models; Blood; Blood Tests; Blood specimen; Cancer Center; Cells; Clinical; Detection; Devices; Diagnosis; Disease Progression; Disease model; ERBB2 gene; Epidermal Growth Factor Receptor; Equipment; Future; Harvest; Health; Heterogeneity; Hour; Human; ITGB3 gene; Label; Letters; Magnetic nanoparticles; Magnetism; Malignant Neoplasms; Measurement; Measures; Methods; Microfluidics; Microspheres; Modeling; Molecular; Molecular Target; Monitor; Mucin 1 protein; Neoplasm Metastasis; Oncogenic; PTPRC gene; Patient Care; Patients; Preparation; Procedures; Proteomics; Sampling; System; TACSTD1 gene; Techniques; Time; Translations; Ultracentrifugation; Western Blotting; Whole Blood; Work; base; biomaterial compatibility; cell type; drug efficacy; gallium arsenide; genetic information; improved; innovation; microchip; microvesicles; multidisciplinary; nanomaterials; nanoparticle; nanoscale; neoplastic cell; protein expression; sensor; specific biomarkers; stem; temporal measurement; tumor

DESCRIPTION (provided by applicant): Tumor cells are known to shed nano-scale objects called circulating microvesicles (CμVs) into patients' blood. These CμVs have been shown to carry molecular information from the tumor, which can potentially be used to diagnose and monitor cancer using only a blood test. However, due to their extremely small size (d ~ 50 nm), there has not been a clinically viable method to detect and profile these CμVs. To address these challenges, we propose a microchip-based platform that can quantitatively profile CμVs directly in unprocessed whole blood. On this chip, we harness the small feature size of microelectronics and combine it with the biocompatibility of microfluidics and magnetic nanoparticles (MNPs) to measure these nano-scale objects. Our proposed device is handheld and aims to reduce measurement times from several hours using conventional equipment to less than thirty minutes. Moreover, due to the high sensitivity of our micro-magnetic sensing method, the biomarker specific limit of detection (100 CμV/mL) will exceed that of conventional techniques. This innovative, clinically practical approach to CμV detection has great potential for non-invasive, routine monitoring of disease progression, drug efficacy, and metastasis, offering tremendous benefits for patients.

描述（由申请人提供）：已知肿瘤细胞会将称为循环微泡（c μ v）的纳米级物体释放到患者的血液中。这些c μ v已被证明携带来自肿瘤的分子信息，可以仅通过血液检查来诊断和监测癌症。然而，由于它们的尺寸非常小（d ~ 50 nm），目前还没有临床可行的方法来检测和描述这些c μ v。为了解决这些挑战，我们提出了一种基于微芯片的平台，可以在未处理的全血中直接定量分析c μ v。在这个芯片上，我们利用微电子的小特征尺寸，并将其与微流体和磁性纳米颗粒（MNPs）的生物相容性相结合，来测量这些纳米级物体。我们提出的设备是手持式的，旨在将使用传统设备的测量时间从几个小时减少到不到30分钟。此外，由于我们的微磁传感方法的高灵敏度，生物标志物的检测特异性限（100 CμV/mL）将超过传统技术。这种创新的、临床实用的CμV检测方法在无创、常规监测疾病进展、药物疗效和转移方面具有巨大的潜力，为患者提供了巨大的益处。

项目成果

Liter-scale production of uniform gas bubbles via parallelization of flow-focusing generators.

• DOI: 10.1039/c7lc00295e
• 发表时间: 2017-07-25
• 期刊: Lab on a chip
• 影响因子: 6.1
• 作者: Jeong HH;Yadavali S;Issadore D;Lee D
• 通讯作者: Lee D

Point-of-care rare cell cancer diagnostics.

• DOI: 10.1007/978-1-4939-2172-0_9
• 发表时间: 2015-01-01
• 期刊: Methods in molecular biology (Clifton, N.J.)
• 作者: Issadore, David

Magnetic Nickel iron Electroformed Trap (MagNET): a master/replica fabrication strategy for ultra-high throughput (>100 mL h(-1)) immunomagnetic sorting.

• DOI: 10.1039/c6lc00487c
• 发表时间: 2016-08-02
• 期刊: Lab on a chip
• 影响因子: 6.1
• 作者: Ko J;Yelleswarapu V;Singh A;Shah N;Issadore D
• 通讯作者: Issadore D

Toolbox for Exploring Modular Gene Regulation in Synthetic Biology Training.

用于探索合成生物学培训中的模块化基因调控的工具箱。

• DOI: 10.1021/acssynbio.6b00057
• 发表时间: 2016
• 期刊: ACS synthetic biology
• 影响因子: 4.7
• 作者: Magaraci,MichaelS;Bermudez,JessicaG;Yogish,Deeksha;Pak,DanielH;Mollov,Viktor;Tycko,Josh;Issadore,David;Mannickarottu,SevileG;Chow,BrianY
• 通讯作者: Chow,BrianY

Hybrid soft-lithography/laser machined microchips for the parallel generation of droplets.

• DOI: 10.1039/c3lc50979f
• 发表时间: 2013-12-21
• 期刊: Lab on a chip
• 影响因子: 6.1
• 作者: Muluneh M;Issadore D
• 通讯作者: Issadore D