Portable optogenetics/two-photon imaging instrument for in vivo brain studies

用于体内脑研究的便携式光遗传学/双光子成像仪器

• 批准号: 9254840
• 负责人: Youbo Zhao
• 金额: $ 22.99万
• 依托单位: PHYSICAL SCIENCES, INC
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-04 至 2018-04-03
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9254840
• 关键词: Achievement; Animal Testing; Animals; Behavior; Brain; Calcium; Complex; Crystallization; Development; Devices; Dimensions; Engineering; Evaluation; Fiber; Fluorescence; Gel; Generations; Genetically Modified Animals; Goals; Government; Hand; Head; Human; Illinois; Image; Imaging Device; Imaging technology; Laboratories; Lasers; Lateral; Light; Lighting; Mechanics; Microscope; Neurons; Neurosciences; Optics; Pattern; Performance; Persons; Phase; Physiologic pulse; Play; Proteins; Research; Research Personnel; Resolution; Sepharose; Source; System; Technology; Testing; Universities; base; cost; density; design; experience; flexibility; fluorescence imaging; in vivo; innovative technologies; instrument; neural circuit; next generation; novel strategies; optical imaging; optogenetics; particle; photonics; physical science; portability; programs; prototype; relating to nervous system; success; tool; two-photon

Project Summary/Abstract Physical Sciences Inc. (PSI) and University of Illinois at Urbana Champaign (UIUC) propose to develop and commercialize an affordable optogenetics/two-photon (2P) imaging instrument that will enable simultaneous manipulation and recording of neuronal activity in genetically modified animals. The goal of this project is to bring this highly desirable tool into the hands of neuroscientists and expand their capability for investigating and deciphering neural circuits underpinning complex brain function and behavior. Currently, the access of neuroscientists to this technology is profoundly limited, because of both high cost, which is often beyond the affordability of neuroscience researchers, and difficulty of use on live animals (bench-top mounted microscope platforms are cumbersome for in vivo brain studies involving living animals). To reach this goal, PSI will use innovative technologies, including dispersion-compensated laser delivery through a fiber cord, coherent supercontinuum (SC) generation in a photonic crystal fiber that enables the use of multiple excitation wavelengths selected for optimal excitation of calcium-sensitive fluorescent proteins, and beam control with spatial light modulators, etc. The achievement of this goal will be supported by the expertise and a proven track record of developing the next generation biomedical devices of the investigators from PSI, as well as the engineering and neuroscience expertise of the leading research groups at University of Illinois at Urbana-Champaign.

项目概要/摘要 物理科学公司 (PSI) 和伊利诺伊大学香槟分校 (UIUC) 提议开发 并将一种经济实惠的光遗传学/双光子 (2P) 成像仪器商业化，这将使 同时操作和记录转基因动物的神经元活动。目标是 该项目旨在将这一非常理想的工具带到神经科学家手中并扩展他们的能力 用于研究和破译支撑复杂大脑功能和行为的神经回路。 目前，由于成本高、 这通常超出了神经科学研究人员的承受能力，并且难以在活体动物上使用 （台式安装的显微镜平台对于涉及活体的体内大脑研究来说很麻烦 动物）。 为了实现这一目标，PSI 将使用创新技术，包括色散补偿激光传输 通过光纤线，在光子晶体光纤中产生相干超连续谱（SC），使得 使用多个激发波长来优化钙敏感荧光的激发 蛋白质，以及空间光调制器的光束控制等。将支持这一目标的实现 凭借开发下一代生物医学设备的专业知识和良好的记录 PSI 的研究人员以及领先研究的工程和神经科学专业知识 伊利诺伊大学厄巴纳-香槟分校的研究小组。