Defining and targeting mechanisms of pancreas cancer pathogenesis

胰腺癌发病机制的定义和靶向机制

• 批准号: 9759649
• 负责人: Martin Whittle
• 金额: $ 13.93万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-19 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9759649
• 关键词: Animal Husbandry; Autopsy; Award; Biology; Complex; Development; Disease; Drug resistance; Enzyme-Linked Immunosorbent Assay; Fluorescence Microscopy; Foundations; Fred Hutchinson Cancer Research Center; Genes; Genetic Engineering; Genotype; Histocytochemistry; Immunotherapy; Laboratories; Laboratory Research; Malignant neoplasm of pancreas; Mass Spectrum Analysis; Modeling; Molecular; Molecular Biology; Molecular Cloning; Mus; Operative Surgical Procedures; Pathogenesis; Postdoctoral Fellow; Process; Quantitative Reverse Transcriptase PCR; Research; Research Project Grants; Senior Scientist; Signal Transduction; Specialist; Techniques; Training; Western Blotting; cancer cell; experimental study; immunoreaction; insight; model development; mouse model; novel therapeutic intervention; programs; tissue culture; tumor; tumor microenvironment

Project Summary Dr. Martin Whittle is a senior scientist working in the laboratory of Dr. Sunil Hingorani at the Fred Hutchinson Cancer Research Center. Dr. Whittle’s training has provided him a strong foundation in the fields of molecular biology, cancer cell signaling, and tumor modeling in mice. This Research Specialist award will support Dr. Whittle’s effort on research projects central to the overall Hingorani Laboratory research program including the following three key projects: the complex pancreas cancer microenvironment and its contributions to disease pathogenesis and drug resistance; the immune reaction in PDA and development of effective immunotherapies; and the molecular underpinnings of the metastatic switch in pancreas cancer. Aside from performing vital experiments for each of these projects, Dr. Whittle further supports the Hingorani lab by training new technicians and postdoctoral fellows in fundamental and advanced laboratory techniques that are critical to the Hingorani research program, including genetic engineering, mouse model development and characterization, animal husbandry, surgical procedures and necropsies, primary tissue culture, histochemistry and immunohistology, genotyping PCR, qRT-PCR, western blotting, FACS, fluorescence microscopy, gene array, molecular cloning, ELISA, mass spectrometry and others.

项目摘要 博士马丁·惠特尔是弗雷德哈钦森的苏尼尔·欣戈拉尼博士实验室的资深科学家 癌症研究中心。惠特尔博士的训练为他在分子生物学领域打下了坚实的基础。 生物学、癌细胞信号传导和小鼠肿瘤建模。该研究专家奖将支持博士。 惠特尔在研究项目上的努力是整个欣戈拉尼实验室研究计划的核心，包括 以下三个关键项目：复杂的胰腺癌微环境及其对疾病的贡献 PDA的发病机制和耐药性; PDA的免疫反应和有效治疗药物的开发 免疫疗法;以及胰腺癌转移开关的分子基础。除了 惠特尔博士为这些项目中的每一个进行了重要的实验，并通过以下方式进一步支持欣戈拉尼实验室： 培训新的技术人员和博士后研究员的基础和先进的实验室技术， Hingorani研究计划的关键，包括基因工程，小鼠模型开发和 表征、动物饲养、外科手术和尸检、原代组织培养、组织化学 免疫组织学、基因分型PCR、qRT-PCR、蛋白质印迹、FACS、荧光显微镜、基因 芯片、分子克隆、ELISA、质谱等。