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Foundations of MRI Corticography for mesoscale organization and neuronal circuitry

中尺度组织和神经元回路的 MRI 皮质成像基础

基本信息

批准号：
9763650
负责人：
David Alan Feinberg
金额：
$ 95.92万
依托单位：
UNIVERSITY OF CALIFORNIA BERKELEY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-09-16 至 2021-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9763650
关键词：
3-Dimensional Accounting Animal Model Area Aricept BRAIN initiative Blood Vessels Brain Brain imaging Brain region Cell Density Cells Cerebral cortex Cholinesterase Inhibitors Computer Simulation Dimensions Distant Electrocorticogram Electrodes Electrophysiology (science)FDA approved Fiber Fluorescence Microscopy Foundations Functional Magnetic Resonance Imaging Goals Human Image Individual Link Magnetic Resonance Imaging Maps Measurable Measures Microscopic Microscopy Modeling Motor Mus Mutant Strains Mice Neuronal Differentiation Neurons Neurosciences Ocular dominance columns Pattern Pharmacology Physiologic pulse Population Research Resolution Rodent Signal Transduction Specific qualifier value Specificity Specimen Surface Technology Testing Transcranial magnetic stimulation Transgenic Mice United States National Institutes of Health Variant Visual Cortex blood oxygenation level dependent response brain circuitry cholinergic density design donepezil frontal lobe functional group hemodynamics high resolution imaging human imaging human model interest millimeter mouse model neuronal circuitry neurovascular next generation novel strategies optogenetics relating to nervous system response species difference temporal measurement

项目摘要

PROJECT SUMMARY Functional MRI (fMRI) is performed at a macroscopic scale of 1 to 3 millimeters spatial resolution. The term `mesoscale' has come to denote the resolution of a finer granularity of neuronal organization, to show functional organization across the depth and along the surface of the cortex. Mesoscale fMRI representation of neural activity, however, is not firmly established. A primary objective of this research is to evaluate fMRI's ability to accurately differentiate neuronal activity in cortical layers and columns. This will allow studies of local circuitry in columnar organization and layers with fiber projections to and from distant brain regions, so that hierarchical and directional connectivity between hundreds of human brain regions may eventually be routinely studied non-invasively in the human brain. This project will leverage state-of-the-art MRI hardware and pulse sequences specifically designed for high- resolution imaging of human cortex in a BRAIN Initiative project for next generation human brain imaging (NIH R24MH106096 ”MRI Corticography” (MRCoG)). It will also use several cutting-edge neuroscience technologies, including CLARITY, optogenetic fMRI (ofMRI), transcranial magnetic stimulation (TMS) and electrocorticography (ECoG), to identify and manipulate neuronal activity underlying the fMRI signal. To determine the spatial specificity and laminar profile of BOLD activity, we will use optogenetic stimulation of neuronal populations in different cortical layers of mouse brain while simultaneously imaging with BOLD fMRI. Secondly, variations of vascular and neuronal density will be disambiguated from variations of co-localized fMRI activity using CLARITY and 3D fluorescence microscopy. In humans, the microscale to mesoscale fMRI mapping will be validated using direct electrophysiological mapping with ultra-high-density ECoG grids and advanced computational modeling. To elucidate whole brain mesoscale circuit interactions in humans, MRCoG will be combined with TMS to test hierarchical organization of frontal cortex and transhemispheric motor connections. In humans, pharmacologically modulated brain circuits will be evaluated using an FDA-approved cholinesterase inhibitor, to determine the laminar profile of mesoscale fMRI when feedforward processing is increased.

项目总结