Vet-LIRN Capacity Building Tissue Digestion

VET-LIRN网络能力建设项目 FOA#：PAR-18-604 印第安纳州动物疾病诊断实验室 普渡大学 项目摘要/摘要 该VET-LIRN网络能力建设项目将促进有效和协调的 FDA CVM Vet-LIRN和印第安纳州对人和动物食品安全问题的回应 普渡大学动物疾病诊断实验室通过提高 通过提供设备齐全和配备人员齐全的毒理学诊断样品制备 实验室。这项能力建设建议的目标是扩大毒理学 实验室消化组织和其他固体诊断样本以进行痕量分析的能力和能力 通过购买两台设备进行矿物和重金属的电感耦合等离子体质谱分析 资助相关技术人员进行消化和分析。这些都很重要 因为这将增加我们调查潜在动物食源性疾病的能力，并使 与Vet-LIRN调查一起进行更有效和协调的分析 紧急增压能力。该项目的具体目标是获得一架菲舍尔布兰德™850 均质机系统，将用于产生样品匀浆以供消化和 提取，微波加速反应系统(MARS6)消化器，将用于 完全消化和准备用于电感耦合等离子体质谱分析的组织和其他固体诊断样品 并为技术人员提供执行均质化、消化和分析的时间。这 该项目将影响动物疾病诊断实验室和Vet-LIRN，因为它将 统一用于电感耦合等离子体/质谱仪分析的样品均质和消解方法 目前正在VET-LIRN合作协议方法资助实验室使用， 能够在大规模动物食品/饲料期间支持突增容量测试 并为短期监测工作提供支持的基础 熟练程度评估。该设备的采购和实施将加强 提高动物疫病诊断实验室的能力，增强我们支持兽医的能力- LIRN在其调查和方法协调努力中，最终达成了一项合作 致力于全面保障食品安全和保护人畜健康。

Vet-LIRN Network Capacity-Building Projects FOA #: PAR-18-604 Indiana Animal Disease Diagnostic Laboratory Purdue University Project Summary/Abstract This Vet-LIRN Network Capacity-Building Project will facilitate an effective and coordinated response to human and animal food safety issues between FDA CVM Vet-LIRN and the Indiana Animal Disease Diagnostic Laboratory at Purdue University through increased capability for diagnostic toxicology sample preparation and by providing for a well-equipped and staffed laboratory. The objective of this Capacity-Building proposal is to expand the toxicology laboratory's capability and capacity to digest tissues and other solid diagnostic samples for trace mineral and heavy metal ICP/MS analyses through the purchase of two pieces of equipment and funding of associated technician time to perform the digestion and analyses. These are important because it will increase our capacity to investigate potential animal foodborne illness and enable more effective and coordinated analyses with Vet-LIRN investigations and in times of emergency surge capacity. The specific aim of this project is to acquire a Fisherbrand™ 850 Homogenizer System which will be used to generate sample homogenates for digestion and extraction, a Microwave Accelerated Reaction System (MARS6) digester which will be used to completely digest and prepare tissues and other solid diagnostic samples for ICP/MS analyses and to fund technician time to perform the homogenizations, digestions and analyses. This project will impact the Animal Disease Diagnostic Laboratory and Vet-LIRN in that it will enable harmonization of sample homogenization and digestion methods for ICP/MS analyses that are currently being used in Vet-LIRN Cooperative Agreement Methods Grant laboratories, afford the ability to support surge capacity testing during large-scale animal food/feed emergency events and provide a foundation of support for short-term surveillance efforts and proficiency evaluations. Acquisition and implementation of this equipment will strengthen the capabilities of the Animal Disease Diagnostic Laboratory and enhance our ability to support Vet- LIRN in its investigatory and methods harmonization efforts, culminating in a collaborative effort toward the overall food safety and protection of human and animal health.