TRD1

建议继续支持荧光动力学实验室扩大规模 加强生物医学技术研究新领域的发现和应用 合作、服务、培训和传播活动，以造福生物医学 研究社区。荧光成像和荧光方法已经达到了 在生物学、生物物理学和临床研究中的突出作用。LFD是一种生物技术 资源中心，在开发新的荧光技术方面取得了成功 生物学和医学，并坚定地致力于服务、培训和传播 旨在将荧光方法引入用户设施的组件，可供 生物医学研究社区。 对于这项提议，我们构思了一个强有力的技术开发计划，以扩大目前的 成像荧光仪器的局限性，特别是对细胞动力学的研究 流程。项目包括：开发用于深部成像的新显微镜平台 组织，仍然保持衍射有限的分辨率；利用光片显微镜 波动分析具有开发激进的新测量方法的潜力 纳米尺度的细胞和组织中的分子流动；使用新的电子学和光学进行3D 细胞动力学成像.扩展相量方法以获得荧光寿命成像 用于动态过程快速、无拟合可视化的频谱分析和扩散分析 并扩展我们的软件，以允许在我们的新的 显微镜。 LFD的组织结构允许用户和协作者访问创新 仅在此BTRR资源中提供的技术，这为他们提供了一个独特的机会 迅速推进自己的研究项目。解决生物问题的丰富程序 开发和推动生物过程、大分子等方面的技术进步和研究 组装、组织组织和动力学、膜形态/功能关系，以及 用于检测血液中细菌的创新生物医学仪器 对临床领域的影响。为该应用程序建议的教育项目包括实践操作 培训学生、博士后研究员和访问科学家、讲习班和专业人员 学校对创新的荧光方法进行了培训。我们改进后的网站将继续成为 有关荧光动力学技术的信息的参考源，以及一个大型存储库 出版物、讲座和教程。

Continuing support is proposed for the Laboratory for Fluorescence Dynamics to expand technological biomedical research to new fields of discovery and applications and to strengthen collaborative, service, training and dissemination activities for the benefit of the biomedical research community. Fluorescence imaging and fluorescence methodologies have reached a prominent role in Biology, Biophysics and in Clinical Research. The LFD is a biotechnological resource center with a history of successes in developing new fluorescence technologies for biology and medicine and with a strong commitment to service, training, and dissemination components aimed at bringing fluorescence methods in a user facility available to the biomedical research community. For this proposal we have conceived a strong technical development program to expand current limits of imaging fluorescence instrumentation, particularly for the study of dynamical cellular processes. Projects include: developments of new microscope platforms for imaging in deep tissues, still maintaining diffraction limited resolution; exploiting the light sheet microscope for fluctuation analysis with the potential for developing radical new approaches to measure molecular flow in cells and tissue at the nanoscale; using new electronics and optics for 3D imaging of cellular dynamics; expanding the phasor approach to fluorescence lifetime imaging analysis to spectral and diffusion analysis for fast, fit-free visualization of dynamical processes and expanding our software to allow new algorithms for data acquisition and analysis in our new microscopes. The organization structure of the LFD allows users and collaborators to access innovative technologies only available in this BTRR resource, which affords them a unique opportunity to rapidly advance their own research programs. A rich program of driving biological problems exploit and push technological advances and research in biological processes, macromolecular assembly, tissue organization and dynamics, membrane morphology/function relationships, and innovative biomedical instrumentation for detection of bacteria in blood which could have an impact in the clinical area. Educational programs proposed for this application include hands-on training of students, postdoctoral fellows and visiting scientists, workshops and specialized schools in innovative fluorescence methodologies. Our improved web site will continue to be a reference source for information about fluorescence dynamic techniques, and a large repository of publications, lectures and tutorials.