Phenotyping and Biobanking Core

项目总结 青少年节律、奖励和睡眠中心(CARRS)表型和生物库核心(核心) B)通过获取具有特定表型的研究对象来支持研究项目 人和动物项目所要求的特征。我们的中心假设是晚睡时间， 睡眠时间短和昼夜节律失调对青少年的皮质边缘功能产生不利影响， 进一步增强奖励敏感性，损害认知控制，增加药物使用风险。这个 协调和深思熟虑的程序，以确保在人类和 动物为这一转化科学计划提供了基础。关于人体研究(项目1和2)， 核心B将对13至15岁至15岁的青少年进行系统的招募和筛选 让参与者系统地选择不同的睡眠时间。对于啮齿动物研究，核心B将建立一个繁殖 和表型鉴定管道，以提供项目3、4和5所需的动物。核心B还将提供 生物库服务，包括收集、管理和保存青少年标本 为测量人类和青春期HS大鼠的分子节律表型并提供高质量的 未来机械学研究的储存库。Core B为这一创新的翻译研究项目做出了贡献 通过协作开发和实施确保CARRS人类相似表型的计划 青少年和青少年啮齿动物模型研究。

PROJECT SUMMARY The Center for Adolescent Rhythms, Reward, and Sleep (CARRS) Phenotyping and Biobanking Core (Core B) supports the research projects through the acquisition of research subjects with specific phenotypic characteristics required by the human and animal projects. Our central hypothesis is that late sleep timing, short sleep duration, and circadian misalignment adversely impact cortico-limbic function in adolescents, further enhancing reward sensitivity, impairing cognitive control, and increasing substance use risk. The coordinated and deliberate procedures for assuring analogous phenotypic characteristics in humans and animals provide the foundation for this translational scientific program. For human studies (Projects 1 and 2), Core B will conduct systematic recruitment and screening of adolescents ages 13 through 15 years old to provide participants systematically varied in sleep timing. For rodent studies, Core B will establish a breeding and phenotyping pipeline to provide the animals required for Projects 3, 4 and 5. Core B will also provide biobanking services, including the collection, management, and banking of specimens from adolescent humans and adolescent HS rats to measure molecular rhythm phenotypes and to provide a high-quality repository for future mechanistic studies. Core B contributes to this innovative translational research program by collaboratively developing and implementing plans for assuring analogous phenotypes for CARRS human adolescent and adolescent rodent model studies.