Core B: Proteomics and lipoprotein characterization core

蛋白质组学和脂蛋白表征核心（核心 B）将提供现代的强大工具 为计划项目资助 (PPG) 研究人员提供质谱分析和复杂数据集分析。核心将 还提供脂蛋白分离以及脂蛋白颗粒浓度和大小的测量 校准的离子淌度分析。核心将允许脂蛋白的结构鉴定和定量 包括残余脂蛋白颗粒 (RLP) 以及 VLDL、LDL 和 HDL，以及测量 使用校准的离子淌度分析计算这些脂蛋白类别的脂蛋白颗粒数。 核心人员精通液相色谱-电喷雾电离-MS/MS分析（鸟枪法 蛋白质的复杂生物混合物的蛋白质组学，特别强调脂蛋白蛋白质组 以及使用靶向 LC-MSMS 分析对其蛋白质货物进行定量。核心总监 建立了 HDL 蛋白质组的精确定量方法并将其应用于 HDL 的定量 蛋白质组在大型转化研究中，并将通过相同的方法应用于其他脂蛋白类别 核心B. 独特的校准离子淌度分析方法测量HDL颗粒数和5个亚种 HDL 颗粒，以及促动脉粥样硬化颗粒（如 RLP）和小而密的 LDL。我们预计所有 调查人员将利用这些功能。 除了提供仪器能力和生物信息学工具外，Core B 工作人员还将提供咨询 以及在质谱和脂蛋白颗粒浓度测量应用方面的合作 计划项目以及复杂数据集的集成和分析。这将包括开发新的 旨在实现该计划研究者的研究目标的分析方法。 核心 B 将优化向 PPG 提供这些服务的效率和成本效益 研究人员通过提供一个中心实验室。这避免了 PPG 个别调查员需要 在自己的实验室维护所需的仪器，避免商业批量的高成本 光谱分析服务，并将确保不同项目地点（西雅图、圣路易斯）分析之间的一致性。 圣路易斯和纽约）。通过集中化和标准化程序，核心 B 将提供一套通用的 分析工具将导致对涉及生理的分子机制的统一理解 和糖尿病血管疾病的病理生理过程。

The proteomics and lipoprotein characterization core (Core B) will provide the powerful tools of modern mass spectrometry and complex data set analysis to Program Project Grant (PPG) investigators. The Core will also provide lipoprotein isolation, and measurements of lipoprotein particle concentration and size using calibrated ion mobility analysis. The Core will permit structural identification and quantitation of lipoproteins including remnant lipoprotein particles (RLPs), as well as VLDL, LDL and HDL, and measurement of lipoprotein particle number of these lipoprotein classes using calibrated ion mobility analysis. Core personnel are proficient in liquid chromatography-electrospray ionization-MS/MS analysis (shotgun proteomics) of complex biological mixtures of proteins, with a particular emphasis on the lipoprotein proteomes and on the quantification of their protein cargo using targeted LC-MSMS analysis. The Core Director established precise methods for quantitation of HDL proteome and applied them to quantification of the HDL proteome in large translational studies and will apply the same methods to other lipoprotein classes through Core B. The unique calibrated ion mobility analysis method measures HDL particle number and 5 subspecies of HDL particles, as well as proatherogenic particles like RLPs, and small dense LDL. We anticipate that all investigators will take advantage of these capabilities. In addition to providing instrumental capabilities and bioinformatics tools, Core B staff will provide consultation and collaboration on the application of mass spectrometry and lipoprotein particle concentration measurements to the Program Project, and integration and analysis of complex datasets. This will include development of new analytical methods targeted at achieving the research objectives of the Program’s investigators. Core B will optimize the efficiency and cost-effectiveness through which these services are provided to PPG investigators by providing a central laboratory. This avoids the need for individual PPG investigators to maintain the required instrumentation in their own laboratories, avoids the high cost of commercial mass spectrometric services, and will ensure consistency between analyses for different project sites (Seattle, St. Louis, and New York). By centralizing and standardizing procedures, Core B will provide a common set of analytical tools that will lead to a unified understanding of molecular mechanisms involved in the physiologic and pathophysiologic processes of diabetic vascular disease.