PROTEOMICS AND BIOINFORMATICS CORE

华盛顿大学糖尿病研究中心的蛋白质组学和生物信息学核心提供 附属研究人员可以使用现代质谱 (MS) 和复杂数据集分析的强大工具。的 核心目标是： (1) 执行 MS 来对复杂的化合物进行公正的发现和半定量分析 使用最先进的非靶向和靶向 LC-MS/MS 技术对蛋白质样品进行定性和定量分析 定量分析，包括开发新的质谱结构鉴定方法； (2) 提供 验证了与糖尿病相关的特定蛋白质分析物的靶向定量分析，以支持临床和 转化研究； (3) 协助DRC附属机构进行研究的实验设计，以确保研究的成功 蛋白质组学分析并提供 MS 原理和使用培训； (4) 提供生物信息学分析 （例如，差异表达分析、蛋白质-蛋白质相互作用网络分析和通路富集 分析）用于蛋白质组和转录组数据集，包括 RNA-seq 和单细胞 RNA-seq，目的 提供糖尿病和糖尿病相关疾病过程的综合系统生物学概述。由 提供进行最先进的蛋白质组学分析所需的集中设施和专业知识， 定量质谱分析和生物信息学分析，核心以最佳方式满足这些目标 支持基础和临床研究的效率和成本效益。此外，通过集中和 通过标准化程序，蛋白质组学和生物信息学核心为其附属研究人员提供了一个共同的 一套分析工具，用于获得对所涉及的分子机制的统一理解 糖尿病的病理生理过程及其相关并发症。

The Proteomics and Bioinformatics Core of the University of Washington Diabetes Research Center provides affiliate investigators the powerful tools of modern mass spectrometry (MS) and complex data set analysis. The goals of the Core are to: (1) Perform MS for unbiased discovery and semi-quantitative analyses of complex protein samples using state-of-the-art untargeted and targeted LC-MS/MS techniques for both qualitative and quantitative analysis, including development of new MS methods for structural identification; (2) Provide validated targeted quantitative assays of specific protein analytes relevant to diabetes in support of clinical and translational studies; (3) Assist DRC affiliates with experimental design of their studies to ensure successful proteomics analysis and provide training in principles and use of MS; and (4) Provide bioinformatics analysis (e.g., differential expression analysis, protein-protein interaction network analysis, and pathway enrichment analysis) for proteomic and transcriptomic data sets, including RNA-seq and single cell RNA-seq, with the aim of providing an integrated, systems biology overview of diabetes and diabetes-related disease processes. By providing a centralized facility and expertise necessary to perform state-of-the-art proteomics analysis, quantitative mass spectrometric assays, and bioinformatics analysis, the Core meets these goals with optimal efficiency and cost-effectiveness in support of basic and clinical research. Further, by centralizing and standardizing procedures, the Proteomics and Bioinformatics Core provides its affiliate investigators a common set of analytical tools for obtaining a unified understanding of molecular mechanisms involved in pathophysiologic processes of diabetes and its associated complications.