Quantitative and Functional Proteomics Core

华盛顿大学糖尿病研究中心的定量和功能蛋白质组学核心 为附属研究人员提供现代质谱分析和复杂数据集分析的强大工具。 核心的目标是： (1) 为 DRC 附属研究人员进行 MS 分析，例如量化 目标分析物并获得用于蛋白质结构鉴定的光谱； (2) 开发新的质谱方法 参与糖尿病及其发病机制的蛋白质的结构鉴定或定量 并发症、危险因素或治疗。； (3) 协助DRC附属机构进行研究实验设计 确保成功的蛋白质组学分析并提供 MS 原理和使用方面的培训； (4) 提供一个中心 数据存储、传播和共享设施； (5) 为分析和分析提供生物信息学支持 解释蛋白质组数据集并将其与基因本体论、蛋白质-蛋白质相互作用相整合 数据库和路径分析； (6) 为蛋白质组学研究的整合提供生物信息学支持 通过功能测定，长期目标是提供糖尿病和糖尿病的综合系统生物学观点 糖尿病相关的疾病过程。通过提供集中式设施，核心可以实现这些目标 最佳效率和成本效益，提供执行最先进的蛋白质组学所需的专业知识 以及当前技术前沿的质谱研究。此外，通过集中和 通过标准化程序，定量和功能蛋白质组学核心为其附属研究人员提供了 一套通用的分析工具，用于获得对所涉及的分子机制的统一理解 糖尿病的病理生理过程及其相关并发症。

The Quantitative and Functional Proteomics Core of the University of Washington Diabetes Research Center provides affiliate investigators the powerful tools of modern mass spectrometry and complex data set analysis. The goals of the Core are to: (1) Perform MS analyses for DRC affiliate investigators, such as quantifying target analytes and obtaining spectra for structural identification of proteins; (2) Develop new MS methods for structural identification or quantification of proteins involved in the pathogenesis of diabetes and its complications, risk factors, or treatment.; (3) Assist DRC affiliates with experimental design of their studies to ensure successful proteomics analysis and provide training in principles and use of MS; (4) Provide a central facility for data storage, dissemination, and sharing; (5) Provide bioinformatics support for analyzing and interpreting proteomic data sets and for integrating them with Gene Ontology, protein-protein interaction databases, and pathway analysis; and (6) Provide bioinformatics support for integration of proteomic studies with functional assays, with the long-term aim of providing an integrated, systems biology view of diabetes and diabetes-related disease processes. By providing a centralized facility, the Core meets these goals with optimal efficiency and cost-effectiveness, providing expertise necessary to perform state-of-the-art proteomics and mass spectrometric studies at the cutting edge of current technology. Further, by centralizing and standardizing procedures, the Quantitative and Functional Proteomics Core provides its affiliate investigators a common set of analytical tools for obtaining a unified understanding of molecular mechanisms involved in pathophysiologic processes of diabetes and its associated complications.