Quantitative and Functional Proteomics Core

定量和功能蛋白质组学核心

• 批准号: 10077858
• 负责人: Tomas Vaisar
• 金额: $ 13.25万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-02-10 至 2022-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10077858
• 关键词: Address; Adipocytes; Animals; Bioinformatics; Biological Assay; Body Fluids; Cells; Data; Data Analyses; Data Set; Data Storage and Retrieval; Databases; Development; Diabetes Mellitus; Disease; Electrospray Ionization; Ensure; Experimental Designs; Flow Cytometry; Functional disorder; Funding; Future; Genes; Goals; Human; Human Resources; Hyperglycemia; Individual; Informatics; Isotopes; Laboratories; Lipoproteins; Liquid Chromatography; Lymph; Mass Spectrum Analysis; Methods; Modernization; Molecular; Monitor; Ontology; Pathway Analysis; Plasma; Preparation; Procedures; Process; Proteins; Proteome; Proteomics; Reaction; Research; Research Personnel; Research Support; Risk Factors; Sampling; Services; Shotguns; Spectrometry, Mass, Electrospray Ionization; Standardization; Structure; Systems Biology; Techniques; Technology; Tissues; Training; Universities; Urine; Washington; analytical method; analytical tool; base; complex data; cost; cost effectiveness; data sharing; diabetes pathogenesis; insight; instrument; instrumentation; interest; islet; liquid chromatography mass spectrometry; macrophage; novel; phenotypic data; protein protein interaction; tandem mass spectrometry; tool; two-dimensional

The Quantitative and Functional Proteomics Core of the University of Washington Diabetes Research Center provides affiliate investigators the powerful tools of modern mass spectrometry and complex data set analysis. The goals of the Core are to: (1) Perform MS analyses for DRC affiliate investigators, such as quantifying target analytes and obtaining spectra for structural identification of proteins; (2) Develop new MS methods for structural identification or quantification of proteins involved in the pathogenesis of diabetes and its complications, risk factors, or treatment.; (3) Assist DRC affiliates with experimental design of their studies to ensure successful proteomics analysis and provide training in principles and use of MS; (4) Provide a central facility for data storage, dissemination, and sharing; (5) Provide bioinformatics support for analyzing and interpreting proteomic data sets and for integrating them with Gene Ontology, protein-protein interaction databases, and pathway analysis; and (6) Provide bioinformatics support for integration of proteomic studies with functional assays, with the long-term aim of providing an integrated, systems biology view of diabetes and diabetes-related disease processes. By providing a centralized facility, the Core meets these goals with optimal efficiency and cost-effectiveness, providing expertise necessary to perform state-of-the-art proteomics and mass spectrometric studies at the cutting edge of current technology. Further, by centralizing and standardizing procedures, the Quantitative and Functional Proteomics Core provides its affiliate investigators a common set of analytical tools for obtaining a unified understanding of molecular mechanisms involved in pathophysiologic processes of diabetes and its associated complications.

华盛顿大学糖尿病研究中心的定量和功能蛋白质组学核心 为附属研究人员提供现代质谱和复杂数据集分析的强大工具。 核心的目标是：（1）为DRC附属研究者进行MS分析，例如量化 目标分析物和获得光谱用于蛋白质的结构鉴定;（2）开发新的MS方法， 糖尿病发病机制中涉及的蛋白质的结构鉴定或定量及其 并发症、危险因素或治疗。(3)协助DRC附属机构进行研究的实验设计， 确保成功的蛋白质组学分析并提供MS原理和使用方面的培训;（4）提供中央 数据存储、传播和共享设施;（5）为分析和 解释蛋白质组学数据集，并将其与基因本体论、蛋白质-蛋白质相互作用 数据库和途径分析;（6）为蛋白质组学研究的整合提供生物信息学支持 与功能测定，长期目标是提供一个综合的，系统生物学观点的糖尿病， 糖尿病相关的疾病过程。通过提供一个集中的设施，核心满足这些目标， 最佳的效率和成本效益，提供执行最先进的蛋白质组学所需的专业知识 和质谱研究的前沿技术。此外，通过集中和 标准化程序，定量和功能蛋白质组学核心提供其附属研究人员， 一套通用的分析工具，用于统一理解 糖尿病及其相关并发症的病理生理过程。