Dissect the Timing Mechanism for Immune Maturation in Plants

项目摘要 生物体的成熟与一系列生理和生物学特性的变化有关。 形态学特征，包括免疫力随年龄的变化。植物经历了一个时代- 在芽成熟过程中，抗病性的依赖性获得，这被称为与抗病性相关的 抗性（ARR）。尽管人们对细胞内的分子和细胞细节有了越来越多的了解， 植物先天免疫网络，我们对防御反应的了解， 在生物体成熟过程中整合到不同的细胞环境中仍然非常有限。 本研究的目的是剖析ARR发病的时间机制 在植物中。我们将利用拟南芥及其细菌病原体假单胞菌pv. 番茄DC 3000作为模式系统，以阐明遗传结构和信号传导 ARR的组成部分。我们的总体假设是，异时microRNA途径是一种 调节发育和免疫成熟的协调启动的内在时钟。 miRNA靶点的功能多样化确保了miRNA靶点的鲁棒性和可塑性。 成熟过程是对内在和环境信号的反应。我们的目标是确定 遗传成分连接发育时间和先天免疫。我们亦会探讨 宿主-病原体对ARR组分的军备竞赛。植物先天免疫系统 在受体结构和信号传导方面与哺乳动物先天免疫系统惊人的相似性 级联。拟议的研究有希望产生新的见解， 发育和免疫程序如何协调的分子机制， 一个有机体的成熟。我们的长期目标是提供基本知识， 发展和防御之间的相互作用，这是植物生存的关键。

PROJECT SUMMARY The maturation of an organism is associated with changes in a spectrum of physiological and morphological traits, including age-dependent change of immunity. Plants undergo an age- dependent gain of disease resistance during shoot maturation, which is known as the Age-related resistance (ARR). Despite the accumulating knowledge of the molecular and cellular details of the plant innate immune network, our knowledge about how the defense responses are incorporate into distinct cellular context during the maturation of an organism is still very limited. The aim of this proposed study is to dissect the timing mechanism that governs the onset of ARR in plants. We will use Arabidopsis thaliana and its bacterial pathogen Pseudomonas syringae pv. tomato DC3000 as a model system to elucidate the genetic architecture and signaling components of ARR. Our overall hypothesis is that a heterochronic microRNA pathway is an intrinsic clock regulating the coordinated onset of developmental and immune maturation. Functional diversification of the miRNA targets ensures the robustness and plasticity of the maturation process in response to intrinsic and environmental signals. We aim to identify the genetic components linking developmental timing and innate immunity. We will also explore the host-pathogen arms race on the components of ARR. The plant innate immune system share striking similarities with the mammalian innate immune system in receptor structure and signaling cascade. The proposed study has promising potential to generate novel insights into the molecular mechanisms of how developmental and immune programs are coordinated during the maturation of an organism. Our long-term goal is to provide fundamental knowledge of the crosstalk between development and defense, a tradeoff critical for plant survival.