Dissect the Timing Mechanism for Immune Maturation in Plants

剖析植物免疫成熟的时间机制

• 批准号: 10672250
• 负责人: Li Yang
• 金额: $ 34.92万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-01 至 2026-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10672250
• 关键词: Age of Onset; Aging; Biological Models; Development; Disease Resistance; Ensure; Genetic; Goals; Health; Immune; Immunity; Innate Immune System; Knowledge; Link; MicroRNAs; Molecular; Morphology; Mouse-ear Cress; Natural Immunity; Organism; Pathway interactions; Pattern; Physiological; Plants; Process; Pseudomonas syringae; Regulation; Resistance; Signal Transduction; Structure; Tomatoes; age related; arms race; defense response; genetic architecture; immune system function; insight; novel; pathogen; pathogenic bacteria; programs; receptor; response; trait

PROJECT SUMMARY The maturation of an organism is associated with changes in a spectrum of physiological and morphological traits, including age-dependent change of immunity. Plants undergo an age- dependent gain of disease resistance during shoot maturation, which is known as the Age-related resistance (ARR). Despite the accumulating knowledge of the molecular and cellular details of the plant innate immune network, our knowledge about how the defense responses are incorporate into distinct cellular context during the maturation of an organism is still very limited. The aim of this proposed study is to dissect the timing mechanism that governs the onset of ARR in plants. We will use Arabidopsis thaliana and its bacterial pathogen Pseudomonas syringae pv. tomato DC3000 as a model system to elucidate the genetic architecture and signaling components of ARR. Our overall hypothesis is that a heterochronic microRNA pathway is an intrinsic clock regulating the coordinated onset of developmental and immune maturation. Functional diversification of the miRNA targets ensures the robustness and plasticity of the maturation process in response to intrinsic and environmental signals. We aim to identify the genetic components linking developmental timing and innate immunity. We will also explore the host-pathogen arms race on the components of ARR. The plant innate immune system share striking similarities with the mammalian innate immune system in receptor structure and signaling cascade. The proposed study has promising potential to generate novel insights into the molecular mechanisms of how developmental and immune programs are coordinated during the maturation of an organism. Our long-term goal is to provide fundamental knowledge of the crosstalk between development and defense, a tradeoff critical for plant survival.

项目总结 生物体的成熟与一系列生理和生理指标的变化有关 形态特征，包括免疫力随年龄的变化。植物经历了一个年龄- 枝条成熟过程中抗病性的依赖获得，这被称为年龄相关 阻力(ARR)。尽管积累了关于分子和细胞细节的知识 植物的先天免疫网络，我们对防御反应的了解 在生物体的成熟过程中融入到不同的细胞环境中仍然是非常有限的。 这项拟议的研究的目的是剖析控制ARR发病的时间机制 在植物中。我们将使用拟南芥及其细菌病原菌紫丁香假单胞菌。 以番茄DC3000为模型系统阐明遗传结构和信号转导 ARR的组件。我们的总体假设是，异时microRNA途径是一种 调节发育和免疫成熟协调开始的内在时钟。 MiRNA靶标的功能多样化确保了 对内在信号和环境信号作出反应的成熟过程。我们的目标是确定 基因成分将发育时间和先天免疫联系在一起。我们还将探索 寄主-病原菌在ARR组分上的军备竞赛。植物的先天免疫系统 受体结构和信号与哺乳动物先天免疫系统惊人的相似之处 卡斯卡德。这项拟议的研究具有潜在的潜力，可以为 发育和免疫程序如何协调的分子机制 生物体的成熟。我们的长期目标是提供有关 发育和防御之间的串扰，这是植物生存的关键权衡。

项目成果

Distinct function of SPL genes in age-related resistance in Arabidopsis.

• DOI: 10.1371/journal.ppat.1011218
• 发表时间: 2023-03
• 期刊: PLoS pathogens
• 影响因子: 6.7

Pathogen-triggered changes in plant development: Virulence strategies or host defense mechanism?

病原体触发的植物发育变化：毒力策略还是宿主防御机制？

• DOI: 10.3389/fmicb.2023.1122947
• 发表时间: 2023
• 期刊: Frontiers in microbiology
• 影响因子: 5.2

Shoot Maturation Strengthens FLS2-Mediated Resistance to Pseudomonas syringae.

芽成熟增强FLS2介导的对丁香假单胞菌的抵抗力。

• DOI: 10.1094/mpmi-02-23-0018-r
• 发表时间: 2023
• 期刊: Molecular plant-microbe interactions : MPMI
• 作者: Hu,Lanxi;Kvitko,Brian;Severns,PaulM;Yang,Li
• 通讯作者: Yang,Li