Effect of CX4945 in tamoxifen resistant BCa

CX4945 在他莫昔芬耐药 BCa 中的作用

• 批准号: 10673636
• 负责人: Christopher Williams
• 金额: $ 28.4万
• 依托单位: XAVIER UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-05 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10673636
• 关键词: Antiestrogen Therapy; Antineoplastic Agents; Breast Carcinoma; Clinical; Degradation Pathway; Development; Diagnosis; Down-Regulation; Endocrine; Endocrine Gland Neoplasms; Estrogen Antagonists; Estrogen Receptor alpha; Estrogen Receptors; Estrogens; Genomics; Goals; Hormones; Incidence; Length; Ligands; Mediating; Metastatic Neoplasm to Lymph Nodes; Modeling; Molecular Chaperones; Mutation; Oncogenic; Patients; Phosphorylation; Phosphotransferases; Play; Predisposition; Protein Isoforms; Proteins; Quality of life; Receptor Signaling; Relapse; Reporting; Repression; Resistance; Role; Selective Estrogen Receptor Modulators; Signal Transduction; Stimulation of Cell Proliferation; Tamoxifen; Testing; Tissue Microarray; Transcript; Ubiquitination; Variant; casein kinase II; efficacious treatment; efficacy evaluation; estrogen disruption; estrogenic; hormone therapy; inhibitor; malignant breast neoplasm; non-genomic; patient derived xenograft model; pharmacologic; preclinical study; prevent; receptor; rho; therapy resistant; tumor xenograft; tumorigenesis

Non-genomic estrogen signaling has been implicated in mediating therapeutic resistance to anti-estrogen therapies in breast carcinoma (BCa) and may contribute to the mitogenic effects of selective estrogen receptor modulators (SERMS). ERα36 [a 36 Kd transcript variant of estrogen receptor alpha (ERα)] mediates non- genomic estrogen signaling, and is implicated in anti-estrogen resistance and anti-estrogen induced mitogenesis1. Dual inhibition of ERα66 (full length ERα) and ERα36 signaling might prove to be a more efficacious mode of antihormonal therapy that avoids the acquisition of anti-estrogen induced resistance and tumorigenesis. This can be achieved by pharmacological targeting of the common posttranslational regulatory mechanisms (ie, phosphorylation) between the two isoforms. CX4945 (silmitasertib), a clinical stage CK2 inhibitor, represses protein levels of both ERα66 and ERα36. As such CX4945 might serve to uniquely disrupt oncogenic kinase signaling as well as reduces stability of ERα isoforms in BCa. We hypothesize that CX4945 inhibits both genomic and nongenomic estrogenic signaling by functioning as an indirect pan-estrogen receptor downregulator which disrupts both ERα66 and ERα36 expression in BCa. In order to test this hypothesis we will: Aim I. Investigate the role of the ERα66/36 /CK2 signaling axis in the development of tamoxifen resistance. For this aim we hypothesize that CK2 promotes the development of 4HT resistance by promoting aberrant ERα signaling. We will determine the incidence and causative role of altered ERα36 and CK2 expression in the development of tamoxifen resistance in breast cancer. Aim 2 Elucidate the mechanisms of CX4945 mediated downregulation of ERα66/36 in BCa. Here we hypothesize that pharmacological inhibition of CK2 with CX4945 renders ERα isoforms susceptible to the 26S proteasomal degradation pathway. We will ascertain the impact of CK2 mediated phosphorylation of ERα66/36 on chaperone interaction, ubiquitination and subsequently the proteolytic degradation of ERα36. Aim 3 Assess the antineoplastic efficacy of CX4945 in paired patient derived xenograft (PDX) models of 4HT sensitive and resistant models of BCa. We hypothesize that CX4945 will inhibit the progression of tamoxifen-sensitive and tamoxifen-resistant (de novo and acquired) PDX tumors. PDX tumors from endocrine sensitive patients will be used to generate models of acquired resistance, whereas de-novo resistance will be modelled using PDX from patients with Y537S mutation. The successful completion of these studies could lead to the use of CX4945 or similar CK2 inhibitors with indirect pan-ERα downregulating functions in the treatment of hormone sensitive and hormone resistant breast cancer.

非基因组雌激素信号已涉及介导抗雌激素治疗抗性

项目成果

Breast Cancer Reconstruction: Design Criteria for a Humanized Microphysiological System.

• DOI: 10.1089/ten.tea.2020.0372
• 发表时间: 2021-02
• 期刊: Tissue engineering. Part A
• 作者: Trivia P Frazier;Christopher Williams;M. Henderson;T. Duplessis;Emma Rogers;Xiying Wu;K. Hamel;E. Martin;O. Mohiuddin;Shahensha Shaik;R. Devireddy;B. Rowan;Daniel Hayes;J. Gimble

NURR1 Is Differentially Expressed in Breast Cancer According to Patient Racial Identity and Tumor Subtype.

• DOI: 10.3390/biomedinformatics2040045
• 发表时间: 2022-12
• 期刊: BioMedInformatics