Research Project 1 - Developing and applying tools to probe internal state dynamics of perception and motivation

研究项目 1 - 开发和应用工具来探测感知和动机的内部状态动态

• 批准号: 10687144
• 负责人: Karl A. Deisseroth
• 金额: $ 83.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-17 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10687144
• 关键词: 3-Dimensional; Animal Behavior; Area; Astronomy; Behavior; Behavioral; Behavioral Model; Biological; Brain; Brain region; Calibration; Cell Nucleus; Cells; Communities; Complex; Data; Data Set; Development; Diameter; Dimensions; Doctor of Philosophy; Education and Outreach; Ensure; Event; Fire - disasters; Gene Expression; Individual; Lead; Learning; Link; Measures; Methods; Mission; Modeling; Molecular; Motivation; Mus; Neuromodulator; Neurons; Noise; Output; Pattern; Perception; Play; Population Dynamics; Positioning Attribute; Preparation; Primates; Probability; Property; Pupil; Reaction Time; Research Project Grants; Resolution; Rodent; Role; Satiation; Sensory; Signal Transduction; Source; Specific qualifier value; Speed; Stimulus; Structure; Technology; Testing; Text; Thirst; Time; Training; Typology; Visual Perception; Work; Writing; alertness; area striata; cell type; data modeling; design; experimental study; interest; laboratory experiment; molecular phenotype; network models; neural; new technology; nonhuman primate; optogenetics; predictive modeling; sensory cortex; sensory input; theories; tool; vigilance; visual stimulus

Research Project 1 - Internal state dynamics of perception and motivati0n Lead: Karl Deisseroth MD PhD Summary This project is focused on development and application of technology for probing the interaction of external sensory input with internal states of the mammalian brain. The unique leverage of our team is technology-driven experimental access to new regimes of speed, scope, and resolution in defining and tuning these internal states. First, we will quantify the population dynamics and statistical structure of time-varying spontaneous activity across the volume of primary visual cortex (with individual cells defined by position and wiring in 3D space, and by molecular identity) to test impact of spontaneous fluctuations on environmental stimulus-triggered neuronal activity and animal behavior. Next, we will quantify cortical activity during transitions among internal states (along with behaviors), impose precisely-defined ensemble activity to assess impact of state transitions on evoked population dynamics and behavior, and quantify endogenous activity influencing these states to assess natural mechanisms and effects. Finally, we will integrate our biological findings and new technological capabilities to directly and precisely impose more complex temporal dynamics of interest by controlling multiple single cells in multiple cortical regions over time. The same cells observed to be active and important in behavior will be made available for molecular phenotyping analysis, so that cell typology may be linked to the specific natural and causal roles of cells in this important domain of circuit computation and behavior.

研究项目 1 - 感知和动机的内部状态动态 负责人：Karl Deisseroth 医学博士 概括 该项目的重点是探索外部相互作用的技术的开发和应用。 哺乳动物大脑内部状态的感觉输入。我们团队的独特优势是技术驱动 在定义和调整这些内部状态时实验性地获得速度、范围和分辨率的新机制。 首先，我们将量化随时间变化的自发活动的种群动态和统计结构 跨越初级视觉皮层的体积（各个细胞由 3D 空间中的位置和连线定义，以及 通过分子身份）来测试自发波动对环境刺激触发的神经元的影响 活动和动物行为。接下来，我们将量化内部状态转换期间的皮质活动 （以及行为），施加精确定义的集成活动来评估状态转换对 诱发群体动态和行为，并量化影响这些状态的内源活动以评估 自然机制和影响。最后，我们将整合我们的生物学发现和新技术 通过控制多个来直接和精确地施加更复杂的感兴趣的时间动态的能力 随着时间的推移，多个皮质区域的单细胞。观察到相同的细胞在行为中活跃且重要 将可用于分子表型分析，以便细胞类型学可以与特定的 细胞在电路计算和行为的这个重要领域中的自然和因果作用。