Ambystoma Genetic Stock Center

钝口螈遗传资源中心

• 批准号: 9212698
• 负责人: Stephen Randal Voss
• 金额: $ 33万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-03-01 至 2020-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9212698
• 关键词: Adolescent; Adult; Advisory Committees; Ambystoma; Ambystoma mexicanum; Applied Research; Area; Behavior; Biological; Biomedical Research; Body part; Brain; Breeding; Cells; Cellular biology; Collection; Communities; Community Services; Computers; Custom; Data; Development; Embryo; Embryonic Development; Ensure; Equipment; Evolution; Funding; Genes; Genetic; Genome; Genomics; Health; Heart; Hour; Human; IACUC; Informatics; Injectable; Injection of therapeutic agent; Institution; International; Jaw; Kentucky; Knock-in; Life; Limb structure; Methods; Microinjections; Mission; Modeling; Monitor; Natural regeneration; Neurobiology; Organ Model; Organism; Physiology; Population; Preparation; Procedures; Production; Protocols documentation; Research; Research Infrastructure; Research Personnel; Research Support; Resources; Retina; Services; Site; Site-Directed Mutagenesis; Speed; Spinal Cord; System; Tail; Time; Tissues; Transgenic Organisms; Translating; United States National Institutes of Health; Universities; Vertebrates; Visit; Work; animal resource; base; experience; gene function; innovation; knowledge base; learning strategy; meetings; operation; organ regeneration; public health relevance; tissue repair; transcription activator-like effector nucleases; web site

DESCRIPTION (provided by applicant): This application seeks support to continue operations of the Ambystoma Genetic Stock Center (AGSC) at the University of Kentucky. The AGSC maintains a historically significant collection of Mexican axolotls (Ambystoma mexicanum), an endangered species that provides living materials in support of biomedical research nationally and internationally. Most notably, axolotls are studied because they are unique among vertebrates in being able to regenerate numerous tissues and body parts. These include the spinal cord, limbs, jaw, retina, brain, heart, and tail, all of which can be regenerated at any poit during embryonic, larval, and adult life. Also, axolotls are used by researchers that work in many additional areas, including neurobiology, ecotoxicology, development, physiology, cell biology, genetics, genomics, and evolution. Over the next 5 years, the AGSC will implement a new resource management plan to better ensure long-term sustainability of axolotl stocks and move each year towards greater self- sufficiency. This will be accomplished by providing high quality stocks that the community has come to depend upon, increasing production and distribution of axolotls, and offering new products and services. In particular, the AGSC will offer a microinjection service to assist investigators in the creation of transgenic axolotls. The AGSC will continue to serve as an informatics hub where investigators obtain information about the collection, technical procedures, potential collaborators, and research findings. Overall, the project will ensure distribution and long-term sustainability of axolotl resources to NIH-funded investigators.

描述（由申请人提供）：本申请寻求支持，以继续在肯塔基大学的Ambystoma遗传库存中心（AGSC）的运营。 AGSC保留了历史上重要的墨西哥axolotls（Ambystoma Mexicanum），这是一种濒临灭绝的物种，可提供用于国家和国际生物医学研究的生存材料。最值得注意的是，研究了Axolotls，因为它们在能够再生许多组织和身体部位的脊椎动物中是独一无二的。其中包括脊髓，四肢，下巴，视网膜，大脑，心脏和尾巴，所有这些都可以在胚胎，幼虫和成人生活期间在任何poit上再生。此外，在许多其他领域工作的研究人员都使用了Axolotls，包括神经生物学，生态毒理学，发育，生理学，细胞生物学，遗传学，基因组学和进化。在接下来的5年中，AGSC将实施一项新的资源管理计划，以更好地确保Axolotl股票的长期可持续性，并每年朝着更大的自给自足发展。这将通过提供社区依赖的高质量股票，增加Axolotls的生产和分配以及提供新产品和服务来实现。特别是，AGSC将提供一项微注射服务，以协助研究人员创建转基因Axolotls。 AGSC将继续作为信息学枢纽，调查人员获得有关收集，技术程序，潜在合作者和研究结果的信息。总体而言，该项目将确保Axolotl资源的分配和长期可持续性向NIH资助的调查人员提供。