Replacement of Pass Through Autoclave Sterilizer Within A High Health Status Barrier Vivarium- Building M3 UC Davis

 描述(由申请人提供)：M3目标基因组学设施隔膜住房单元是加州大学戴维斯分校(UC Davis)校园内唯一的高健康状况屏障啮齿动物住房设施，迫切需要更换专用散装直通高压灭菌器。M3屏障间隔室的生物安全取决于这一关键设备的运行功能，该设备已有40多年的历史，已被评估和证明具有不可修复的损害，这将导致显著的设备故障和随后的设施运行故障，危及其中饲养的动物的健康和福利。M3设施为加州大学戴维斯分校的38名调查人员提供积极的拨款和内部校园支持，总额约为1050万美元。所有用品、笼子、床上用品、饲料和瓶子都经过高压灭菌处理，以确保M3屏障隔膜中饲养的转基因动物和免疫受损啮齿类动物的严格健康状况。对于几个由NIH资助的大型赠款储存库项目(突变小鼠资源和研究中心、KnoKout小鼠项目库和小鼠代谢表型中心的减肥外科模型分发)，M3屏障间隔膜充当了高健康“供应商状态屏障”。M3屏障住房设施还容纳了为加州大学戴维斯分校校园啮齿动物健康监测计划提供所有啮齿动物哨兵动物的校园聚居地。因此，M3屏障设施的运营影响范围超出了设施内饲养的动物范围，并影响到加州大学戴维斯分校所有与鼠标相关的研究。目前的高压灭菌器已有40多年的历史，已经超过了预期寿命。由于缺乏基础设施建设，几年来蒸汽供应不足造成的损坏导致高压灭菌器护套出现裂缝，无法修复。正在申请资金，以更换通过高压灭菌器的散装灭菌器，并为新设备提供额外的专用电动锅炉支助，以防止中央蒸汽基础设施不足造成的过度磨损。新的高压灭菌器将更加节能、节水，并提高运营效率，以支持M3靶向基因组学隔膜设备的日益增长的使用。这将确保当前和未来啮齿动物项目的生物安全、福利和健康，取决于健康状况 这个隔间收容所提供的。

 DESCRIPTION (provided by applicant): The M3 Targeted Genomics Facility Vivarium Housing unit is the only high health status barrier rodent housing facility on the University of California Davis (UC Davis) Campus and is in dire need of replacement of the facility dedicated bulk pass-through autoclave sterilizer. The biosecurity of the M3 barrier vivarium is dependent on the operational function of this critical piece of equipment which is over 40 years old and has been assessed and demonstrated to have irreparable damage which will lead to eminent equipment failure and subsequent facility operational failure jeopardizing the animal health and welfare of those animals housed within. The M3 facility supports 38 UC Davis investigators with active grant and internal campus support totaling approximately $10.5 million. All supplies, caging, bedding, feed, and bottles are autoclaved into the facility to ensure the strict health status of the genetically modified animals and immune compromised rodent colonies housed in the M3 barrier vivarium. The M3 barrier vivarium serves as the High health "vendor status barrier" for shipping associated with several large NIH funded grant repository projects (Mutant Mouse Resource and Research Center, Knockout Mouse Project Repository, and the Bariatric Surgical Model Distribution for the Mouse Metabolic Phenotyping Center). The M3 barrier housing facility also houses the campus colony which supplies all rodent sentinel animals for the UC Davis Campus Rodent Health Monitoring Program. Thus, the impact of the operations of the M3 barrier facility reach beyond the animals housed within the facility and affect all mouse related research across the UC Davis campus. The current autoclave is over 40 year old and has been used past its life expectancy. Damage from several years of inadequate steam supply due to lack of infrastructure development have led to cracks in the jacket of the autoclave which can't be repaired. Funds are being requested for replacement of the bulk pass through autoclave sterilizer as well as additional dedicated electric boiler support for the new equipment to prevent excessive wear and tear from inadequate central steam infrastructure. The new autoclave will be more energy efficient, water saving, and improve operational efficiency in order to support the growing use of the M3 Targeted Genomics Vivarium Facility. This will ensure the biosecurity, welfare, and health of current and future rodent projects reliant on the health status that this vivarium housing facility provides.