Acquisition of rodent metabolic and behavioral phenotyping system

啮齿动物代谢和行为表型系统的获取

• 批准号: 10799014
• 负责人: Kyle Patrick Quinn
• 金额: $ 25万
• 依托单位: UNIVERSITY OF ARKANSAS AT FAYETTEVILLE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2026-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10799014
• 关键词: Arkansas; Behavioral; Bioenergetics; Biomedical Research; Body Weight; Carbon Dioxide; Cells; Centers of Research Excellence; Consumption; Data; Data Science; Development; Disease; Faculty; Future; Genus Hippocampus; Image; Interdisciplinary Study; Isotopes; Kinetics; Laboratory Animals; Measurement; Metabolic; Metabolism; Molecular; Monitor; Oxygen Consumption; Phase; Production; Research; Research Personnel; Research Project Grants; Resolution; Resources; Rodent; Role; System; Tissues; Universities; behavioral phenotyping; innovation; instrument; laboratory experiment; metabolic phenotype; recruit; repaired; water vapor

PROJECT SUMMARY The Arkansas Integrative Metabolic Research Center (AIMRC) is a Phase I COBRE at the University of Arkansas at Fayetteville (04/01/2021-02/28/2026; PI: Dr. Kyle P. Quinn). The scientific objective of the AIMRC is to investigate the role of cell and tissue metabolism in disease, development, and repair through research involving advanced imaging, bioenergetics, and data science. The long-term objective of the AIMRC is to establish a sustainable interdisciplinary research center that can support biomedical research at the U of A and grow the emerging strengths in metabolic research on campus. The Bioenergetics Core currently assists researchers with measurements of metabolic flux at the cell and isolated tissue levels. This supplement will add complementary new capabilities by enabling non-destructive and automated monitoring of rodents to simultaneously monitor body weight, oxygen consumption, carbon dioxide release, and water vapor formed during the kinetic activities of these laboratory animals through the acquisition of Promethion metabolic cages. The instrument will enable investigators to assess the unusual consumption/accumulation of heavy isotope enriched (13C/15N/2H) metabolites formed under various disease conditions. The data acquired using the Promethion metabolic core is expected to provide valuable information to understand the molecular mechanisms underlying various diseases. The information gained from the Promethion metabolic core can be highly complementary to the data acquired using the other available instrumentational facilities (Seahorse and Oroboros respirometers) available in the AIMRC Bioenergetics Core. The Promethion metabolic cages purchased as part of this supplement request will provide a unique resource to our campus and region. It will be highly beneficial for the research proposed by current research project leaders and recruited new faculty who will be candidates for future advancement to research project leaders.

项目摘要 阿肯色州综合代谢研究中心（AIMRC）是阿肯色州大学的一期COBRE 在费耶特维尔（04/01/2021-02/28/2026; PI：凯尔P.奎因博士）。AIMRC的科学目标是 研究细胞和组织代谢在疾病，发育和修复中的作用， 先进的成像、生物能量学和数据科学。AIMRC的长期目标是建立一个 可持续的跨学科研究中心，可以支持生物医学研究在A的U和增长 代谢研究的新兴优势。生物能量学核心目前帮助研究人员 在细胞和分离的组织水平的代谢通量的测量。这一补充将补充 新的能力，使非破坏性和自动监测啮齿动物，同时监测 体重、耗氧量、二氧化碳释放量和运动过程中形成的水蒸气 这些实验室动物通过收购异丙嗪代谢笼。该仪器将使 研究人员评估重同位素富集（13 C/15 N/2 H）的异常消耗/积累 在各种疾病条件下形成的代谢物。使用异丙嗪代谢核心获得的数据 有望提供有价值的信息，以了解各种潜在的分子机制， 疾病从异丙嗪代谢核心获得的信息可以与数据高度互补 使用其他可用的仪器设备（Seahorse和Oroboros呼吸计）获得 在AIMRC生物能量学核心。作为本补充剂的一部分购买的异丙嗪代谢笼 请求将提供一个独特的资源，我们的校园和地区。这将是非常有益的研究 由目前的研究项目负责人提出，并招募了新的教师谁将是未来的候选人 晋升为研究项目负责人。