Acquisition of rodent metabolic and behavioral phenotyping system

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PROJECT SUMMARY The Arkansas Integrative Metabolic Research Center (AIMRC) is a Phase I COBRE at the University of Arkansas at Fayetteville (04/01/2021-02/28/2026; PI: Dr. Kyle P. Quinn). The scientific objective of the AIMRC is to investigate the role of cell and tissue metabolism in disease, development, and repair through research involving advanced imaging, bioenergetics, and data science. The long-term objective of the AIMRC is to establish a sustainable interdisciplinary research center that can support biomedical research at the U of A and grow the emerging strengths in metabolic research on campus. The Bioenergetics Core currently assists researchers with measurements of metabolic flux at the cell and isolated tissue levels. This supplement will add complementary new capabilities by enabling non-destructive and automated monitoring of rodents to simultaneously monitor body weight, oxygen consumption, carbon dioxide release, and water vapor formed during the kinetic activities of these laboratory animals through the acquisition of Promethion metabolic cages. The instrument will enable investigators to assess the unusual consumption/accumulation of heavy isotope enriched (13C/15N/2H) metabolites formed under various disease conditions. The data acquired using the Promethion metabolic core is expected to provide valuable information to understand the molecular mechanisms underlying various diseases. The information gained from the Promethion metabolic core can be highly complementary to the data acquired using the other available instrumentational facilities (Seahorse and Oroboros respirometers) available in the AIMRC Bioenergetics Core. The Promethion metabolic cages purchased as part of this supplement request will provide a unique resource to our campus and region. It will be highly beneficial for the research proposed by current research project leaders and recruited new faculty who will be candidates for future advancement to research project leaders.