Acquisition of rodent metabolic and behavioral phenotyping system

啮齿动物代谢和行为表型系统的获取

• 批准号: 10799014
• 负责人: Kyle Patrick Quinn
• 金额: $ 25万
• 依托单位: UNIVERSITY OF ARKANSAS AT FAYETTEVILLE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2026-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10799014
• 关键词: Arkansas; Behavioral; Bioenergetics; Biomedical Research; Body Weight; Carbon Dioxide; Cells; Centers of Research Excellence; Consumption; Data; Data Science; Development; Disease; Faculty; Future; Genus Hippocampus; Image; Interdisciplinary Study; Isotopes; Kinetics; Laboratory Animals; Measurement; Metabolic; Metabolism; Molecular; Monitor; Oxygen Consumption; Phase; Production; Research; Research Personnel; Research Project Grants; Resolution; Resources; Rodent; Role; System; Tissues; Universities; behavioral phenotyping; innovation; instrument; laboratory experiment; metabolic phenotype; recruit; repaired; water vapor

PROJECT SUMMARY The Arkansas Integrative Metabolic Research Center (AIMRC) is a Phase I COBRE at the University of Arkansas at Fayetteville (04/01/2021-02/28/2026; PI: Dr. Kyle P. Quinn). The scientific objective of the AIMRC is to investigate the role of cell and tissue metabolism in disease, development, and repair through research involving advanced imaging, bioenergetics, and data science. The long-term objective of the AIMRC is to establish a sustainable interdisciplinary research center that can support biomedical research at the U of A and grow the emerging strengths in metabolic research on campus. The Bioenergetics Core currently assists researchers with measurements of metabolic flux at the cell and isolated tissue levels. This supplement will add complementary new capabilities by enabling non-destructive and automated monitoring of rodents to simultaneously monitor body weight, oxygen consumption, carbon dioxide release, and water vapor formed during the kinetic activities of these laboratory animals through the acquisition of Promethion metabolic cages. The instrument will enable investigators to assess the unusual consumption/accumulation of heavy isotope enriched (13C/15N/2H) metabolites formed under various disease conditions. The data acquired using the Promethion metabolic core is expected to provide valuable information to understand the molecular mechanisms underlying various diseases. The information gained from the Promethion metabolic core can be highly complementary to the data acquired using the other available instrumentational facilities (Seahorse and Oroboros respirometers) available in the AIMRC Bioenergetics Core. The Promethion metabolic cages purchased as part of this supplement request will provide a unique resource to our campus and region. It will be highly beneficial for the research proposed by current research project leaders and recruited new faculty who will be candidates for future advancement to research project leaders.

项目总结 阿肯色州综合代谢研究中心(AIMRC)是阿肯色州大学的一期工程 在费耶特维尔(2021年4月1日-2026年2月28日；PI：Kyle P.Quinn博士)。AIMRC的科学目标是 通过以下研究探讨细胞和组织代谢在疾病、发育和修复中的作用 先进的成像、生物能量学和数据科学。AIMRC的长期目标是建立一个 可持续的跨学科研究中心，可以支持加州大学的生物医学研究，并发展 在校园新陈代谢研究方面的新优势。生物能量学核心目前协助研究人员 在细胞和隔离组织水平上的代谢流量的测量。这份补充文件将补充 通过实现对啮齿动物的非破坏性和自动化监控来同时监控 在动力学活动中形成的体重、氧气消耗、二氧化碳释放和水蒸气 这些实验动物通过收购扑灭净代谢笼子。该仪器将使 调查人员评估重同位素浓缩物(13C/15N/2H)的异常消耗/积累 在各种疾病条件下形成的代谢物。使用扑灭净代谢核心获得的数据 有望提供有价值的信息来理解各种不同的分子机制 疾病。从扑灭净代谢核心获得的信息可以与数据高度互补 使用其他可用的仪器设备(海马和奥罗博罗斯呼吸仪)获取 在AIMRC生物能量学核心。作为本补充材料的一部分购买的扑灭净代谢笼 申请将为我们的校园和地区提供独特的资源。这将对这项研究大有裨益 由现任研究项目负责人提出，并招聘将成为未来候选人的新教师 晋升为研究项目负责人。