FUNCTIONS OF THE BROTHER/CBFB PROTO-ONCOPROTEIN FAMILY

兄弟/CBFB 原癌蛋白家族的功能

• 批准号: 2561036
• 负责人: John Peter Gergen
• 金额: $ 18.27万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2561036
• 关键词: DNA binding protein; Drosophilidae; animal genetic material tag; animal tissue; chimeric proteins; chromosome deletion; circular dichroism; developmental genetics; embryonic stem cell; gene dosage; gene duplication; gene interaction; genetic regulatory element; microcalorimetry; nuclear magnetic resonance spectroscopy; oncoproteins; phenotype; protein structure function; protooncogene; regulatory gene; sedimentation equilibrium; stoichiometry; transcription factor; transposon /insertion element

The proposed study concerns two families of proteins that interact to form a heteromeric transcription regulator, CBF (Core Binding Factor). This factor was first studied in its capacity as a regulator of Moloney murine leukemia virus and Polyoma DNA tumor virus function. In mammals, it is required for the development of hematopoietic lineages, and the genes encoding both the a and ss subunits of the factor are frequent targets for chromosomal rearrangements associated with leukemia in humans. CBFa is a DNA binding protein, while CBFss interacts with a, increasing its DNA binding affinity. ss itself cannot bind DNA. (Dr. Banerjee's lab) and in mouse embryonic stem cells homozygous for a loss of function CBFss mutation (Dr. Speck's lab). The Drosophila studies will utilize Bro, BGB and CBFss genes placed into GAL4-responsive UAST P element transformation vectors. Gal4 expression will be directed by maternal, eye and heat shock promoters. With the isolation of Bro mutations, the ss proteins will be examined for interchangeability in mutation rescue experiments. If that is successful, studies of chimeric protein functions will also be carried out.

这项提议的研究涉及两个相互作用形成a蛋白的蛋白质家族