FORMATION OF LITHIATED ADDUCTS OF GLYCEROPHOSPHOCHOLINE LIPIDS FACILITATES
促进甘油磷酸胆碱脂质的锂加合物的形成
基本信息
- 批准号:6665786
- 负责人:
- 金额:$ 15.75万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2002
- 资助国家:美国
- 起止时间:2002-08-01 至 2003-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Electrospray ionization (ESI) tandem mass spectrometry (MS) has
simplified analysis of phospholipid mixtures, and, in negative ion
mode, permits structural identification of picomole amounts of
phospholipid species. Collisionally activated dissociation (CAD) of
phospholipid anions yields negative ion tandem mass spectra that
contain fragment ions representing the fatty acid substituents as
carboxylate anions. Glycerophosphocholine (GPC) lipids contain a
quaternary nitrogen moiety and more readily form cationic adducts than
anionic species, and positive ion tandem mass spectra of protonated
GPC species contain no abundant ions which identify fatty acid
substituents. We report here that lithiated adducts of GPC species
are readily formed by adding lithium hydroxide to the solution in
which phospholipid mixtures are infused into the ESI source. CAD of
[MLi+] ions of GPC species yields tandem mass spectra that contain
prominent ions representing losses of the fatty acid
sub stituents. These ions and their relative abundances can be used
to assign the identities and positions of the fatty acid substituents
of GPC species. Tandem mass spectrometric scans monitoring neutral
losses of the head-group or of fatty acid substituents from lithiated
adducts can be used to identify GPC species in tissue phospholipid
mixtures. Similar scans monitoring parents of specific product ions
can also be used to identify the fatty acid substituents of GPC
species, and this facilitates identification of distinct isobaric
contributors to ions observed in the ESI/MS total ion current.
电喷雾电离(ESI)串联质谱(MS)具有
磷脂混合物的简化分析,和,在负离子
模式,允许对皮摩尔量进行结构鉴定
磷脂种类。 碰撞活化解离(CAD)
磷脂阴离子产生负离子串联质谱,
含有代表脂肪酸取代基的碎片离子,
羧酸根阴离子。 甘油磷酸胆碱(GPC)脂质含有
季氮部分和更容易形成阳离子加合物比
质子化的阴离子种类和正离子串联质谱
GPC物种不含丰富的识别脂肪酸的离子
取代基。 我们在这里报告,GPC物种的锂化加合物
通过向溶液中加入氢氧化锂,
该磷脂混合物被注入ESI源。 的计算机辅助设计
[MLi GPC物质的+]离子产生串联质谱,其包含
代表脂肪酸损失的主要离子
替代品 这些离子和它们的相对丰度可用于
指定脂肪酸取代基的身份和位置
GPC物种 串联质谱扫描监测中性
从锂化中损失头基或脂肪酸取代基
加合物可用于鉴定组织磷脂中的GPC物质
混合物。 类似扫描监测特定产物离子的母体
也可用于鉴定GPC的脂肪酸取代基
物种,这有利于识别不同的等压
在ESI/MS总离子电流中观察到的离子的贡献者。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('FONG-FU HSU', 18)}}的其他基金
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体内生物转化新代谢物的表征
- 批准号:
8361399 - 财政年份:2011
- 资助金额:
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ELECTROSPRAY IONIZATION WITH LOW-ENERGY COLLISIONALLY ACTIVATED DISSOCIATION
低能碰撞激活解离的电喷雾电离
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STRUCTURAL CHARACTERIZATION OF UNSATURATED GLYCEROPHOSPHOLIPIDS
不饱和甘油磷脂的结构表征
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ELUCIDATION OF THE DOUBLE-BOND POSITION OF LONG-CHAIN UNSATURATED FATTY ACIDS
长链不饱和脂肪酸双键位置的阐明
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- 资助金额:
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ELECTROSPRAY IONIZATION WITH LOW-ENERGY COLLISIONALLY ACTIVATED DISSOCIATION
低能碰撞激活解离的电喷雾电离
- 批准号:
8168803 - 财政年份:2010
- 资助金额:
$ 15.75万 - 项目类别:
CHARACTERIZATION OF NEW METABOLITES FROM IN VIVO BIOTRANSFORMATION
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- 批准号:
8168802 - 财政年份:2010
- 资助金额:
$ 15.75万 - 项目类别:
ELUCIDATION OF THE DOUBLE-BOND POSITION OF LONG-CHAIN UNSATURATED FATTY ACIDS
长链不饱和脂肪酸双键位置的阐明
- 批准号:
7954004 - 财政年份:2009
- 资助金额:
$ 15.75万 - 项目类别:
STRUCTURAL CHARACTERIZATION OF PHOSPHATIDYL-MYO-INOSITOL MANNOSIDES FROM
磷脂酰肌醇甘露糖苷的结构表征
- 批准号:
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- 资助金额:
$ 15.75万 - 项目类别:
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不饱和甘油磷脂的结构表征
- 批准号:
7954003 - 财政年份:2009
- 资助金额:
$ 15.75万 - 项目类别:
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