900 MHz NMR Spectrometer for Structural Biology

描述：纽约结构生物学中心是一家由代表九个研究机构的董事会管理的501(C)(3)公司，建议购买一台900 MHz核磁共振光谱仪用于结构生物学研究，并为光谱仪的成本寻找艺术液体。首席调查员和其他调查员将分享这一资源的使用，并将鼓励其他用户。即将进行的研究包括蛋白质和核酸的结构测定、动力学研究、蛋白质折叠、同位素标记方法的发展、高相对分子质量的应用以及膜蛋白质的应用。这些研究是旨在了解结构生物学的基本科学问题和许多发育疾病、癌症、肌肉骨骼疾病、神经疾病和传染病涉及的生理和病理过程的计划的一部分。研究人员包括使用核磁共振的领先结构生物学家，以及该领域的领先实验家和方法开发人员。这种丰富的智力环境将为科学生产力、创造力和使用拟议仪器的协作带来丰硕成果。900兆赫光谱仪将被安置在1万平方米的空间内。如果。目前正在建设中的扩建项目，与纽约广播公司现有的实验室毗邻。研究人员将可以使用800 MHz和更低的场强仪器，以便900 MHz系统可以有选择地用于需要超高场的实验者。S对超高场的具体要求包括应用于使用横向优化弛豫谱(TROSY)及其“”导数的大分子体系，用于测量剩余偶极耦合的磁取向，以及依赖于场的CSA、动力学和其他弛豫相关现象。

DESCRIPTION: The New York Structural Biology Center, a 501(c) (3) corporation governed by a Board representing nine research institutions, proposes to purchase a 900 MHz NMR spectrometer for research in structural biology, and seeks fluids for art of the Cost of the spectrometer. The principal investigators and other investigators will share in the use of this resource, and will encourage other users. Research to be constructed includes structure determination of proteins and nucleic acids, dynamics studies, protein folding, and development of methods of isotopic labeling, application to higher molecular weights, and application to membrane proteins. These studies are part of programs aimed at understanding both basic scientific problems in structural biology and at physiological and pathological processes involved in many diseases of development, cancer, muscle skeletal disorders, neurological diseases, and infectious diseases. The investigators include leading structural biologists using NMR, and leading experimentalists and methods developers in the area. This rich intellectual environment will be fruitful for scientific productivity, creativity, and collaboration using the proposed instrument. The 900 MHz spectrometer will be housed in a 10,000 sq. if. extension now in construction, contiguous with the NYSBC's existing laboratory. The investigators will have access to' 800 MHz and lower field instruments, so that the 900 MHz system Can be used selectively for those experimentalists requiring ultra high field. Specific requirement S for ultra high field include applications to large molecular weight systems using transverse optimize relaxation spectroscopy (TROSY) and its ''derivatives, magnetic orientation for measurement of residual dipolar couplings, and field-dependent CSA, dynamics, and other relaxation-related phenomena.