A Vervet Monkey (Cercopithecus Aethiops) Genetic Map

黑长尾猴（Cercopithecus Aethiops）遗传图谱

• 批准号: 6651577
• 负责人: NELSON B. FREIMER
• 金额: $ 28.48万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6651577
• 关键词: Cercopithecidae; artificial chromosomes; behavioral /social science research tag; behavioral genetics; ethology; fluorescent in situ hybridization; gene expression; genetic library; genetic mapping; genetic markers; genetic polymorphism; genome; genotype; linkage mapping; maternal behavior; phenotype; polymerase chain reaction; population genetics; quantitative trait loci; sex behavior; social behavior

DESCRIPTION (provided by applicant): This is a proposal to develop an integrated genetic resource for investigations of complex traits in the vervet monkey (Cercopithecus aethiops), also known as the African Green monkey. Studies of non-human primates are invaluable for furthering understanding of a wide range of human diseases and traits, especially complex behaviors. The utility of such studies derives from the fact that humans are much more similar to other primates than they are to commonly used mammalian model organisms such as the mouse. The vervet is widely used in biomedical research, particularly for investigation of complex behavioral traits. A captive population at the UCLA-VA Vervet Monkey Research Colony (VMRC) contains a large number of normally reared animals in an extended six-generation pedigree, with most relationships verified by DNA marker typing. Sub-sections of this pedigree will be genotyped to construct two related genetic maps. The first map consists of about 600 human microsatellite markers that cover most of the human genome, and that have already been shown to be polymorphic in vervets under standard genotyping conditions. The relative location of these markers in the vervet genome will be confirmed by multipoint linkage analyses and fluorescence in situ hybridization (FISH). Additional markers will be chosen to minimize the number of large gaps (greater than 15 cM) in the genetic map. A complementary map will consist of up to 2000 single nucleotide polymorphisms (SNPs) drawn from the 3? end of genes. These maps will be used by the scientific community for genetic mapping studies of complex traits in the VMRC pedigree and in vervet populations sited at several other locations. A large insert clone library that we will construct will anchor the genetic maps to the vervet genome and will facilitate the identification of genes that are implicated in predisposition to particular vervet traits. This library will also be useful for investigators who wish to study use of a vervet model to study particular candidate genes identified from investigations of humans.

描述（由申请人提供）：这是一个开发 用于研究长尾猴复杂性状的综合遗传资源 猴（Cercopithecus aethiops），也被称为非洲绿色猴。 对非人灵长类动物的研究对于进一步了解 广泛的人类疾病和特征，特别是复杂的行为。的 这些研究的实用性来自于这样一个事实，即人类更相似， 而不是通常使用的哺乳动物模式生物， 就像老鼠一样。长尾猴广泛用于生物医学研究，特别是 研究复杂的行为特征一个圈养的人口在 UCLA-VA长尾猴研究群（VMRC）包含大量的 通常饲养的动物在一个延长的六代血统，与大多数 通过DNA标记分型验证的关系。这一谱系的子部分将 进行基因分型以构建两个相关的遗传图谱。第一张地图包括 大约600个人类微卫星标记，覆盖了人类基因组的大部分， 已经证明在标准条件下， 基因分型条件。这些标记在长尾猴体内的相对位置 基因组将通过多点连锁分析和荧光检测来确认。 原位杂交（FISH）。将选择其他标记，以最大限度地减少 遗传图谱中的大缺口（大于15 cM）的数量。互补 一份由2000个单核苷酸多态性（SNPs）组成的图谱 从3？基因的终结这些地图将被科学界使用 用于VMRC谱系和中复杂性状的遗传作图研究， 在其他几个地点的黑斑羚种群。一个大的插入克隆 我们将构建的基因库将把基因图谱锚定在长尾猴上， 基因组，并将有助于识别基因，涉及 易受某些黑猩猩特征的影响这个图书馆也会很有用 对于希望研究使用长尾猴模型来研究特定 通过对人类的研究发现了候选基因。