MOLECULAR BASIS OF PERSISTENT EHRLICHIA INFECTION

持续性埃里克体感染的分子基础

• 批准号: 6695578
• 负责人: XUE-JIE YU
• 金额: $ 26.08万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-01 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6695578
• 关键词: Ehrlichia; bacterial genetics; bacterial proteins; dogs; ehrlichiosis; emerging infectious disease; epitope mapping; intracellular parasitism; membrane proteins; microorganism immunology; nucleic acid sequence; polymerase chain reaction; recombinant proteins; ticks

DESCRIPTION (Adapted from the Applicant's Abstract): Persistent infection has been observed for all ehrlichial species in a variety of animals and in humans. Understanding the mechanism of persistent ehrlichial infection will enable design of new therapeutic approaches to treat ehrlichial infections and vaccines to prevent the diseases. The following hypotheses are presented: 1) persistent infection is caused by antigenic variation and/or differential expression of the outer membrane protein (OMP) genes of Ehrlichia and 2) the OMPs confer immunity in the host against re-infection by the pathogen. The subject of this proposal is Ehrlichia chaffeensis which causes a newly emerging infectious disease, human monocytotropic ehrlichiosis. Persistent infection by E. chaffeensis has been documented in human and animals. A canine model will be employed to study the mechanism of this persistent infection. In specific aim 1, antigenic variation of E. chaffeensis gp120 will be analyzed in dogs infected by tick transmission. Canine blood will be obtained over a six month period, the gp120 gene will be amplified by PCR and sequenced and compared to the gene in the infecting isolate to determine the possible deletion or insertion of repeats in the gp120 gene. In addition, changes in the size and antigenicity of gp120 reisolated from infected canine blood will be examined. Specific aim 2 will entail analysis of the differential expression of the p28 multigene family. This will be accomplished by RT-PCR or epitope mapping. Aim 3 will involve testing of the protective immunity elicited in canines by immunization with recombinant OMPs, followed by challenge of immunized animals with E. chaffeensis. This will provide valuable information regarding the potential use of these antigens as subunit vaccines.

描述（改编自申请人摘要）：持续性感染有

项目成果

L-selectin and E-selectin expressed on monocytes mediating Ehrlichia chaffeensis attachment onto host cells.

L-选择素和E-选择素在单核细胞上表达，介导恰菲埃里希体附着到宿主细胞上。

• DOI: 10.1016/s0378-1097(03)00696-7
• 发表时间: 2003
• 期刊: FEMS microbiology letters
• 影响因子: 2.1
• 作者: Zhang,Jian-zhi;McBride,JereW;Yu,Xue-jie
• 通讯作者: Yu,Xue-jie

Expression of members of the 28-kilodalton major outer membrane protein family of Ehrlichia chaffeensis during persistent infection.

查菲埃里希体 28 千道尔顿主要外膜蛋白家族成员在持续感染过程中的表达。

• DOI: 10.1128/iai.72.8.4336-4343.2004
• 发表时间: 2004
• 期刊: Infection and immunity.
• 作者: Zhang,Jian-zhi;Guo,Hong;Winslow,GaryM;Yu,Xue-jie
• 通讯作者: Yu,Xue-jie

Ehrlichia prevalence in Amblyomma americanum, Central Texas.

德克萨斯州中部美洲钝眼埃利希体流行率。

• DOI: 10.3201/eid1007.03-0792
• 发表时间: 2004
• 期刊: Emerging infectious diseases
• 影响因子: 11.8
• 作者: Long,ScottWesley;Pound,JMathews;Yu,Xue-jie
• 通讯作者: Yu,Xue-jie

Quantitation of human astrovirus by real-time reverse-transcription-polymerase chain reaction to examine correlation with clinical illness.

通过实时逆转录聚合酶链反应定量人类星状病毒，以检查与临床疾病的相关性。

• DOI: 10.1016/j.jviromet.2006.01.009
• 发表时间: 2006
• 期刊: Journal of virological methods.
• 作者: Zhang,Zhian;Mitchell,DouglasK;Afflerbach,Carol;Jakab,Ferenc;Walter,Jolan;Zhang,Yan-Jin;Staat,MaryA;Azimi,Parvin;Matson,DavidO
• 通讯作者: Matson,DavidO