Testing Measures of Maternal and Infant Circadian Rhythm

母婴昼夜节律检测措施

• 批准号: 6806795
• 负责人: KAREN A. THOMAS
• 金额: $ 7.58万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6806795
• 关键词: actigraphy; analytical method; behavior test; biomarker; body physical activity; child behavior; circadian rhythms; clinical research; computer data analysis; data collection methodology /evaluation; environmental adaptation; enzyme linked immunosorbent assay; experimental designs; human ecology; human subject; infant human (0-1 year); melatonin; method development; mother child interaction; parents; patient oriented research; sample collection; urinalysis; urine; videotape /videodisc

DESCRIPTION (provided by applicant): One of the most important infant developmental accomplishments is fitting into the family life pattern, which is driven by circadian and diurnal (day-night) rhythms. Mothers serve an essential role in entraining infant circadian rhythm, synchronizing the immature infant circadian rhythm with the 24-hour day. The application extends current research supported by a career development award. The purpose of the proposed methodological study is to test infant and maternal actigraphy and infant urinary 6-sulfatoxymelatonin (6-sMT) as markers of circadian rhythm. Aims include determining the sensitivity threshold setting for computer scored actigraphy in infants, developing methods and protocols for performing actigraphy in mothers and infants collecting infant urinary 6-sulfatoxymelatonin in the home environment, testing the laboratory assay of infant urinary 6-sulfatoxymelatonin collected from diapers, and estimating the number of continuous days of actigraphy recording required to accurately portray circadian rhythm of mother and infant rest-activity pattern. The study will be conducted in two phases, each employing an intensive within subject design and including healthy term gestation infants and their mothers. In Phase 1, laboratory-based actigraphy will be conducted in ten mother-infant pairs over a two-hour period in conjunction with live observation and videotaping. Correspondence between live observation of activity and computer scored actigraphy will be assessed over a range of activity thresholds to define appropriate software sensitivity setting for the study of infants. In Phase 2, maternal-infant actigraphy will be conducted over a four-day period in the home. Infant diapers will also be collected over a 24-hour period. Urine samples will be extracted and urinary 6-sMT assayed. Prior to the home study, the diaper extraction procedures and ELISA assay will be tested by (1) instilling known concentrations of assay standard in diapers, extracting samples, and evaluating results against the assay standard curve, (2) examining differences between 6-sMT values from urine tested with and without diaper extraction. Circadian rhythm parameters (mesor, amplitude, acrophase based on cosinor analysis) will be calculated using one through four days of data to ascertain a data collection period that accurately portrays infant and maternal rest-activity pattern. Throughout both Phases mother's acceptability of study procedures will be assessed. Methods, refined in the low risk population, will subsequently be employed in the study of maternal entrainment of preterm infants.

描述（由申请人提供）：婴儿最重要的发育成就之一是融入家庭生活模式，这是由昼夜节律和昼夜节律驱动的。母亲在引导婴儿昼夜节律、使未成熟的婴儿昼夜节律与一天 24 小时同步方面发挥着重要作用。该申请扩展了由职业发展奖支持的当前研究。所提出的方法学研究的目的是测试婴儿和母亲的体动记录仪和婴儿尿 6-磺酰氧基褪黑激素 (6-sMT) 作为昼夜节律的标志物。目的包括确定计算机评分婴儿体动记录仪的灵敏度阈值设置，开发在家庭环境中收集婴儿尿 6-磺酰氧基褪黑素的母亲和婴儿中进行体动记录仪的方法和方案，测试从尿布收集的婴儿尿 6-磺酰氧基褪黑素的实验室测定，并估计准确描述所需的体动记录仪连续天数。 母亲和婴儿休息活动模式的昼夜节律。该研究将分两个阶段进行，每个阶段都采用强化主题设计，包括健康足月婴儿及其母亲。在第一阶段，将在两小时内对十对母婴进行基于实验室的体动记录，并结合现场观察和录像。将在一系列活动阈值上评估活动的实时观察和计算机评分的体动记录仪之间的对应关系，以确定用于婴儿研究的适当的软件灵敏度设置。在第二阶段，将在家中进行为期四天的母婴体动记录。婴儿尿布也将在 24 小时内收集。将提取尿液样本并检测尿液 6-sMT。在家庭研究之前，将通过以下方式测试尿布提取程序和 ELISA 测定：(1) 将已知浓度的测定标准滴入尿布中，提取样品，并根据测定标准曲线评估结果，(2) 检查有和没有尿布提取的尿液中 6-sMT 值之间的差异。将使用一到四天的数据计算昼夜节律参数（基于余弦分析的中值、振幅、末相），以确定准确描绘婴儿和母亲休息活动模式的数据收集周期。在这两个阶段中，将评估母亲对学习程序的可接受性。在低风险人群中完善的方法随后将用于早产儿母体诱导的研究。