Smart ventilated cage systems for next-level experimental design and monitoring of specialized animal models.

智能通风笼系统，用于下一级实验设计和专门动物模型的监测。

• 批准号: 10737361
• 负责人: BRIAN KAROLEWSKI
• 金额: $ 33.8万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10737361
• 关键词: Address; Animal Experiments; Animal Housing; Animal Model; Animal Technicians; Animal Welfare; Animals; Area; Attention; Beds; Behavior; Biology; Breeding; Cancer Model; Caring; Censuses; Circadian Rhythms; Clinical; Collection; Communicable Diseases; Complement; Complex; Darkness; Data; Data Collection; Data Correlations; Dedications; Disease; Disease Progression; Disease model; Early identification; Electrodes; Ensure; Environmental Monitoring; Experimental Designs; Fertility; Floods; Food; Generations; Genetic; Health; Health Status; House mice; Housing; Human; Humidity; Image; Individual; Infection; Investments; Knockout Mice; Light; Lighting; Link; Locomotion; Maintenance; Mammals; Masks; Medical center; Medicine; Modeling; Monitor; Mouse Strains; Mus; Phenotype; Physiology; Play; Precision therapeutics; Preclinical Testing; Predisposition; Prevention strategy; Procedures; Protocols documentation; Reproducibility; Research; Research Personnel; Research Project Grants; Research Support; Resource Sharing; Resources; Sampling; Schedule; Services; Site; Standardization; Stress; Symptoms; System; Temperature; Testing; Time; Transgenic Organisms; United States National Institutes of Health; Universities; Veterinarians; Water; animal facility; animal resource; behavioral study; circadian; comparative; digital; experimental study; human disease; improved; member; mouse model; novel; operation; patient derived xenograft model; pre-clinical; precision oncology; prevent; programs; remote monitoring; sensor; treatment response; treatment strategy; ventilation; vibration; wasting; web interface; welfare

PROJECT SUMMARY Murine models play a key role in a broad range of research areas given their comparable physiology to humans and other mammals, known genetic backgrounds, and the ease of their housing and maintenance. However, as we have gained a greater understanding of physiology, there has been a drive to create more sophisticated models that better represent human disease progression and response to treatments. This has led to the generation and use of mouse models with unique genetic backgrounds, behaviors, or susceptibility to diseases. While these models have been of enormous value, they require regular monitoring and may need special housing conditions (such as altered light/dark cycles) to maintain their health and successfully carry out needed research. Incorporating this monitoring adds significant operational burden on facility staff and researchers, and may also add to animal stress. Furthermore, even with increased physical monitoring of mice, important changes in health status can be missed during non-observational periods, making this a challenging problem to address. To address these issues, the Institute of Comparative Medicine (ICM) at Columbia University Irving Medical Center will acquire and install Tecniplast Digital Ventilated Cages (DVC) and Rack Environmental Monitoring modules for its specialized animal resources. The features of the DVC system will improve ICM operations by: 1) Providing automated, 24/7 remote monitoring of various parameters linked to animal welfare and environmental conditions of the animal facility; 2) triggering of automated alarms in particular conditions requiring immediate attention (e.g., decreased spontaneous mouse activity, water flooding, limited water bottle or food availability); 3) setting specific light/dark cycle for individual cages with the use of LEDDY units; and 4) reducing the number of cage changes needed in some circumstances (e.g. single-housed mice), reducing bedding waste. Implementation of DVC cages will be targeted toward specialized shared resources that utilize the most complex disease models, which will lead to a disproportionately large impact on animal welfare, facility operations, experimental capability of researchers, and the reproducibility and rigor of research within ICM.

项目摘要 鉴于其可比的生理学与 人类和其他哺乳动物，已知的遗传背景以及其住房和维护的便利性。 但是，随着我们对生理学有了更深入的了解，已经有一个动力来创造更多 更好地代表人类疾病进展和对治疗反应的复杂模型。这就是 导致具有独特遗传背景，行为或易感性的小鼠模型的生成和使用 疾病。尽管这些模型具有巨大的价值，但它们需要定期监控，并且可能需要 特殊的住房条件（例如变化的光/黑暗周期）以维持其健康并成功执行 需要研究。合并此监控为设施工作人员和 研究人员，也可能增加动物压力。此外，即使对小鼠的身体监测增加， 在非守护时期可能会错过健康状况的重要变化，这使得这是一个具有挑战性的 问题要解决。 为了解决这些问题，哥伦比亚大学欧文医学的比较医学研究所（ICM） 中心将获取和安装Tecniplast数字通风笼（DVC）和机架环境监控 专门动物资源的模块。 DVC系统的功能将通过： 1）提供自动化的24/7远程监视与动物福利链接的各种参数和 动物设施的环境条件； 2）在特定条件下触发自动警报 需要立即注意（例如，自发的小鼠活动减少，水灾，水瓶有限 或食物可用性）； 3）使用LEDDY单元设置特定的光/黑暗周期；和4） 减少在某些情况下（例如单身小鼠）所需的笼子变化的数量，减少 床上用品浪费。 DVC笼的实施将针对使用最多的专业共享资源 复杂的疾病模型，这将导致对动物福利，设施的影响不成比例 ICM内的运营，研究人员的实验能力以及研究的可重复性和严格性。