MEALS OF VARYING FAT & FIBER CONTENT EFFECT ON POSTPRANDIAL TESTOSTERONE CONC

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 饮食和生活方式的改变可以改善多囊卵巢综合征(PCOS)的症状，包括高雄激素血症和月经紊乱。然而，还没有研究评估患有多囊卵巢综合征的女性对不同饮食结构的膳食的餐后反应。主要目的是确定不同脂肪和纤维含量的膳食是否会影响患有多囊卵巢综合征的女性的血浆睾酮浓度。第二个目标是观察不同脂肪和纤维含量的膳食是否会影响循环中的葡萄糖、胰岛素和促性腺激素(促黄体生成素和卵泡刺激素)浓度。我们将招募患有多囊卵巢综合征(慢性少/无排卵和高雄激素血症)的19-40岁女性，她们没有服用任何混合药物(激素避孕药、胰岛素敏感剂、抗高血压药、抗精神病药物)或目前正在怀孕。所有受试者都将完成知情同意书和病史，并接受体检、双能X射线吸收测量(DXA)和经阴道超声检查。受试者将在15分钟内吃到测试餐。这顿饭要么包括：(1)鸡蛋奶酪牛角面包和全脂牛奶，要么(2)谷类食品，低脂牛奶和一个苹果或香蕉，酸奶。每餐后每10分钟采血3毫升，连续6小时测定促卵泡激素和促黄体生成素，在30分钟和每小时采血20毫升检测葡萄糖、胰岛素、睾酮和SHBG。血糖水平将使用MiniMed连续血糖监测系统进行持续监测。参与者将参加这两种条件下的测试用餐，间隔至少七天。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Changes in diet and lifestyle can improve symptoms of polycystic ovary syndrome (PCOS), including hyperandrogenemia and menstrual disturbances. However, no studies have evaluated the postprandial response to meals of varying diet composition in women with PCOS. The primary objective is to determine if meals of different fat and fiber content will affect plasma testosterone concentrations in women with PCOS. The second objective is to see if meals of different fat and fiber content affect circulating glucose, insulin and gonadotropin (LH and FSH) concentrations. We will recruit women age 19-40 with polycystic ovary syndrome (chronic oligo/anovulation and hyperandrogenemia) who are not taking any confounding medications (hormonal contraceptives, insulin sensitizing agents, antihypertensives, antipsychotics) or are currently pregnant. All subjects will complete an informed consent and medical history and undergo a physical exam, dual energy x-ray absorptiometery (DXA) and transvaginal ultrasound. Subjects will be served the test meal that is to be consumed within 15 minutes. The meal will either consist of: (1) egg and cheese croissant and whole milk or (2) cereal, low-fat milk and an apple or banana, yogurt. After each meal, 3-mL blood samples will be taken every 10 minutes for 6 hours for FSH and LH measures and at 30 minutes and every hour, 20 mL will be taken for glucose, insulin, testosterone and SHBG. Glucose levels will be monitored continuously using the MiniMed Continuous Glucose Monitoring System. Participants will participate in both conditions with test meals being separated by at least seven days.