DARTMOUTH COL COBRE: P1: REGULATION OF BIOFILM DEVELOPMENT ON EPITHELIAL CELLS

达特茅斯 COL COBRE：P1：上皮细胞生物膜发育的调节

• 批准号: 7610603
• 负责人: George A. O'Toole
• 金额: $ 24.21万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610603
• 关键词: Bacteria; Clinical; Computer Retrieval of Information on Scientific Projects Database; Development; Environment; Epithelial Cells; Funding; Gene Proteins; Genes; Grant; Growth; Institution; Microbial Biofilms; Molecular Profiling; Morphology; Physiology; Regulation; Regulatory Pathway; Reporting; Research; Research Personnel; Resources; Role; Signal Transduction; Source; Surface; System; United States National Institutes of Health; Work; microbial community; protein expression; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Biofilms are surface-attached microbial communities found in clinical, industrial and natural environments. Numerous studies suggest that biofilm bacteria can be distinguished from their planktonic counterparts in terms of morphology and physiology, and also differ in gene and protein expression profiles. We have proposed that the formation of biofilms is a well-regulated developmental system. If biofilm formation is analogous to other developmental systems we hypothesize that there should be distinct regulatory pathways that control the transition between planktonic and biofilm growth, and this regulatory cascade should be controlled in response to environmental signals. In the original proposal we described studies of the SadARS three-component regulatory system. We have reported those studies previously. We have extended this work to include several other genes with key roles in early biofilm development, including SadC and BifA. Our progress on these newly discovered genes is outlined below.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 生物膜是临床、工业和自然环境中发现的表面附着的微生物群落。大量研究表明，生物膜细菌在形态和生理学方面可以与浮游细菌区分开来，并且在基因和蛋白质表达谱上也有所不同。我们提出生物膜的形成是一个调节良好的发育系统。如果生物膜形成与其他发育系统类似，我们假设应该存在不同的调节途径来控制浮游生长和生物膜生长之间的过渡，并且应该根据环境信号来控制这种调节级联。在最初的提案中，我们描述了 SadARS 三部分监管系统的研究。 我们之前已经报道过这些研究。 我们已将这项工作扩展到包括在早期生物膜发育中发挥关键作用的其他几个基因，包括 SadC 和 BifA。 我们在这些新发现的基因方面取得的进展概述如下。