DEVELOPMENT OF A HAND-HELD FLOW CYTOMETER: HIV DIAGNOSIS

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目的目标是开发低成本的便携式流式细胞仪用于生物医学应用。这些仪器将在许多领域产生巨大影响，包括医学（艾滋病毒和癌症诊断），国土防御（生物点检测，生物监视和法医分析）和一般生物医学研究（配体受体研究，分子组装分析，高通量筛选和基因分型）。许多强大的应用使流式细胞仪成为几乎每个大学，医学院，制药公司和诊断实验室的固定设备。 然而，大量消耗品的尺寸、费用和要求限制了它们在正式实验室环境中的使用。 低成本便携式流式细胞仪将使所有临床医生和研究人员都能使用这些应用，这将对世界卫生和研究产生重大影响。 为了开发这样的仪器，我们将开发（在R21阶段）技术，允许粒子聚焦，而不使用鞘液。 鞘液的消除将导致仪器的便携性增加，并且还减少消耗品需求。我们的新技术将允许无鞘便携式流式细胞仪实现常规的颗粒分析速率，同时使用传统流式细胞仪的一小部分功率和耗材。 我们还将开发低功率激发/检测系统（R21阶段），不需要广泛的温度调节来实现光学测量稳定性。这样的系统将显著减小流式细胞仪的尺寸、成本和功率要求。 最后，我们将把上述技术整合到一个小型的、经济实惠的流式细胞仪中，它具有先进的样品处理能力，大大降低了耗材和功耗要求（R33阶段）。 为此，我们将联合收割机将上述技术结合到微型流式细胞仪核心中，该核心可以轻松与样本处理和光学模块连接。我们还将开发一个微型低功耗数据采集系统，使组装一个完整的微型低功耗流式细胞仪。我们将使用定量流式细胞术技术验证仪器性能。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this project is to develop low cost portable flow cytometers for use in biomedical applications. Such instruments would have a large impact in many areas including medicine (HIV and cancer diagnosis), homeland defense (biological point detection, bio-surveillance, and forensic analysis) and general biomedical research (ligand-receptor studies, molecular assembly analysis, high throughput screening and genotyping). The many powerful applications have made flow cytometers a fixture in nearly every university, medical school, pharmaceutical company, and diagnostic lab. However, the size, expense and requirement for large amounts of consumables have limited their use to formal laboratory settings. A low cost portable flow cytometer would make these applications available to all clinicians and researchers, which would have a great impact on world health and research. To develop such an instrument we will develop (in the R21 phase) technologies that allow particle focusing without the use of sheath fluid. The elimination of sheath fluid will result in increased portability of the instrument and also reduce consumable requirements. Our novel technology will allow a sheathless portable flow cytometer to achieve conventional particle analysis rates, while using a fraction of the power and consumables of a conventional flow cytometer. We will also develop low power excitation/detection systems (R21 phase) that will not require extensive thermoregulation to achieve optical measurement stability. Such systems, will significantly reduce the size, cost and power requirements of a flow cytometer. Finally, we will integrate the above technologies into a miniature affordable flow cytometer, with advanced sample handling capabilities that has significantly reduced consumable and power requirements (R33 phase). To do this we will combine the above technologies into a microfabricated flow cytometer core that can easily be interfaced with sample handling and optical modules. We will also develop a miniature low power data acquisition system to enable assembly of a complete miniature low power flow cytometer. We will validate instrument performance using quantitative flow cytometry techniques.