STRUCTURAL DETERMINATION OF PROTEINS IN ARABIDOPSIS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 真核结构基因组学中心的主要目标是发展和关键分析高通量、蛋白质组规模、真核蛋白质的生产、鉴定和结构确定的方法。这项技术正在通过测定拟南芥蛋白质的晶体和溶液状态三维结构进行测试和改进。特别强调其序列可能包含新折叠的蛋白质、与新功能相关的蛋白质、或可能具有已知折叠但具有先前未与该折叠相关的功能的蛋白质。据设想，大多数结构将由X射线结晶学确定，核磁共振用于更小、更具活力的蛋白质和无法结晶的更小的蛋白质靶标。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The major aims of the Center for Eukaryotic Structural Genomics are the development and critical analysis of methods for high-throughput, proteome-scale, eukaryotic protein production, characterization, and structure determination. This technology is being tested and refined through the determination of crystal and solution state three-dimensional structures of proteins from Arabidopsis thaliana. Particular emphasis is placed on proteins whose sequences suggest that they may contain a novel fold, proteins associated with novel functions, or proteins likely to have a known fold but with a function not previously associated with that fold. It is envisioned that most of the structures will be determined by X-ray crystallography, with NMR used for smaller, more dynamic proteins and for smaller protein targets that fail to crystallize.