MICROPROBE/MICROFLUORESCENCE DEVELOPMENT

这个子项目是众多研究子项目之一

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have performed the following development related to the biocat microprobe: new KB mirrors were installed and commissioned, previous mirrors were damaged and their reflectivity was extremely low. New mirrors ordered form Xradia were installed and tested. Mirrors performance was measured in terms of beam size and flux delivered. Each mirror showed more than 80 % efficiency and minimum focus obtained was 3 microns x 3 microns FWHM. A new solid state detector was also tested. The detector is a SDD detector 100 mm^2 active area (ten times larger than the regular SDD detector). The resolution of the new detector is 170 eV at the Mn kalfa line. The Saturn electronics from XIA was optimized for high count rates up to 200 kcps. The scanning software was improved to minimize the overhead time down to 0.5 sec per point. The combination of the new detector and the improved scanning software allows us to obtain fluorescence maps in about a third of the time. Typical scanning times are 3-4 hours now. A new sample holder system was developed to allow three samples to be mounted on the scanning stages. The system also includes a rotary stage for tomography experiments. A new set of calibration standard from NIST have been included in the set of standards for calibration purposes.