MICROPROBE/MICROFLUORESCENCE DEVELOPMENT

显微探针/微荧光显影

• 批准号: 7601773
• 负责人: RAUL BARREA
• 金额: $ 5.87万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7601773
• 关键词: Area; Calibration; Computer Retrieval of Information on Scientific Projects Database; Computer software; Count; Development; Electronics; Fluorescence; Funding; Grant; Hour; Institution; Maps; Measures; Performance; Purpose; Rate; Research; Research Personnel; Resolution; Resources; Sampling; Saturn; Scanning; Source; Staging; Standards of Weights and Measures; System; Testing; Time; United States National Institutes of Health; detector; improved; research study; size; solid state; tomography

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have performed the following development related to the biocat microprobe: new KB mirrors were installed and commissioned, previous mirrors were damaged and their reflectivity was extremely low. New mirrors ordered form Xradia were installed and tested. Mirrors performance was measured in terms of beam size and flux delivered. Each mirror showed more than 80 % efficiency and minimum focus obtained was 3 microns x 3 microns FWHM. A new solid state detector was also tested. The detector is a SDD detector 100 mm^2 active area (ten times larger than the regular SDD detector). The resolution of the new detector is 170 eV at the Mn kalfa line. The Saturn electronics from XIA was optimized for high count rates up to 200 kcps. The scanning software was improved to minimize the overhead time down to 0.5 sec per point. The combination of the new detector and the improved scanning software allows us to obtain fluorescence maps in about a third of the time. Typical scanning times are 3-4 hours now. A new sample holder system was developed to allow three samples to be mounted on the scanning stages. The system also includes a rotary stage for tomography experiments. A new set of calibration standard from NIST have been included in the set of standards for calibration purposes.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们进行了以下与biocat微探针相关的开发：安装并调试了新的KB反射镜，以前的反射镜被损坏，反射率极低。安装并测试了从Xradia订购的新镜子。反射镜的性能测量的光束大小和通量传递。每个反射镜显示出超过80%的效率，并且获得的最小焦点为3微米X 3微米FWHM。还测试了一种新的固态探测器。探测器为SDD探测器，有效面积为100 mm^2（比常规SDD探测器大10倍）。新探测器在Mn kalfa线上的分辨率为170 eV。XIA的Saturn电子器件针对高达200 kcps的高计数率进行了优化。 改进了扫描软件，将开销时间降至每点0.5秒。新探测器和改进的扫描软件的结合使我们能够在大约三分之一的时间内获得荧光图。现在典型的扫描时间是3-4小时。开发了一种新的样品保持器系统，允许将三个样品安装在扫描台上。该系统还包括一个用于层析成像实验的旋转台。NIST的一套新的校准标准品已被纳入校准标准品组中。