MICROPROBE/MICROFLUORESCENCE DEVELOPMENT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们进行了以下与Biocat微探头相关的开发：安装并调试了新的KB镜，以前的镜被损坏，其反射率极低。从XRadia订购的新镜子被安装和测试。反射镜的性能是根据光束大小和提供的光通量来衡量的。每个反射镜的效率都在80%以上，获得的最小焦距为3微米×3微米半高宽。还测试了一种新的固态探测器。该探测器是一个100 mm^2的SDD探测器(比常规的SDD探测器大十倍)。新探测器在锰卡尔法谱线上的分辨率为170 eV。来自XIA的土星电子设备针对高达200KCPS的高计数速率进行了优化。对扫描软件进行了改进，将开销时间降至每点0.5秒。新的探测器和改进的扫描软件相结合，使我们能够在大约三分之一的时间内获得荧光图谱。现在典型的扫描时间是3-4个小时。开发了一种新的样品保持器系统，允许三个样品安装在扫描工作台上。该系统还包括一个用于断层成像实验的旋转工作台。NIST的一套新的校准标准已包括在该套标准中，用于校准目的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have performed the following development related to the biocat microprobe: new KB mirrors were installed and commissioned, previous mirrors were damaged and their reflectivity was extremely low. New mirrors ordered form Xradia were installed and tested. Mirrors performance was measured in terms of beam size and flux delivered. Each mirror showed more than 80 % efficiency and minimum focus obtained was 3 microns x 3 microns FWHM. A new solid state detector was also tested. The detector is a SDD detector 100 mm^2 active area (ten times larger than the regular SDD detector). The resolution of the new detector is 170 eV at the Mn kalfa line. The Saturn electronics from XIA was optimized for high count rates up to 200 kcps. The scanning software was improved to minimize the overhead time down to 0.5 sec per point. The combination of the new detector and the improved scanning software allows us to obtain fluorescence maps in about a third of the time. Typical scanning times are 3-4 hours now. A new sample holder system was developed to allow three samples to be mounted on the scanning stages. The system also includes a rotary stage for tomography experiments. A new set of calibration standard from NIST have been included in the set of standards for calibration purposes.