VIBRATIONALLY COOLED MATRIX-ASSIST LASER DESORPTION/IONIZATION FT MASS SPECTROM

振动冷却基质辅助激光解吸/电离 FT 质谱

基本信息

  • 批准号:
    7601951
  • 负责人:
  • 金额:
    $ 3.88万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2007
  • 资助国家:
    美国
  • 起止时间:
    2007-08-03 至 2008-05-31
  • 项目状态:
    已结题

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The improvement and optimization of the Vibrationally Cooled MALDI-FTMS continues. This year we carried out further testing an evaluation of the XY stage that was designed and constructed in collaboration with the Fraunhofer Institute. This stage is a critical component for full automation of the MALDI-FTMS. The design parameters for this stage were defined to handle the typical proteomic type sample. Many proteomic experiments use robots that are designed to work with microtiter plates which are ~76 x 89 mm in size. Therefore, the stage was required to have full travel of at least 100 x 100 mm. For the MALDI Fourier transform mass spectrometer used, the travel time from spot to spot is not the limiting factor in the experiment speed, so a travel of 2 mm/sec was defined as it would allow the stage to position to the next spot 1 mm away in 0.5 seconds. The positioning accuracy was defined to be 1 um, which is much more accurate than is needed for the standard MALDI experiment in which the laser beam spot is often 50 um diameter, but will allow the stage to be used in a MALDI imaging mode. Aside from this, the stage had to be truly vacuum compatible, had to be mounted vertically, and to generate no detectable RF interference noise, and had to take up minimal space so that the vacuum chamber could be made as small as possible for improved pumping performance. The two-dimensional stage is a single block design with the two axis motors, linear encoders, and position switches all integrated into the central block. The system uses ballscrews and crossed roller bearings which will require minimal lubrication in vacuum; what little lubrication is needed is provided by low vapor pressure diffusion pump oil. In order to build the motors into this block, they are mounted on copper brackets (to dump their heat into the block which acts as a heat sink) and pulleys are used to transfer their torque to the ballscrew. The belts for these pulleys are actually small electrowelded stainless steel bands, and in order to keep them centered on the pulleys, the pulley wheels are ground to be slightly concave. Tensioning set screws are mounted into the block to allow the pulleys to be tightened properly during testing. In order to prevent over determination of the position of the ballscrew, it is mounted in two places only: the pulley bearing assembly and the plate. The stage itself is designed in three pieces, an upper and lower plate, and the central block. The motors, limit switches, and linear encoder electronics are all mounted (and heat sinked) to the central block. The linear encoder slides are mounted to the upper and lower plates. The three pieces are black anodized to improve surface hardness, but even more importantly, to improve radiative cooling of the blocks. All wire routes are also contained within the block. The motors and electronics dump their heat loads into the central block, which is only able to dissipate that heat through conductive cooling at the bearings and radiative cooling. The conductive cooling is very inefficient since the bearings are stainless steel (very low heat transfer capacity) and the contact area is very low. A copper band or braid was considered for improving the conductive heat transfer rate, but a quick calculation of the heat transfer of such a band compared to the radiative cooling rate showed that the heat transfer through the copper band was negligible. There are several positioning screws for the crossed roller bearings, wire traces, and bolt holes for mounting the motors, bearings, and electronics. All of these holes are vented to minimize virtual leaks. The system uses four position encoders, two linear encoders for determining position, and two rotary encoders mounted on the back of the motors for determining velocity. The system uses optical limit switches. The switch is a photodiode/phototransistor pair mounted on the central stage body which is interrupted by an aluminum flag mounted on the upper and lower stage plates. Two such switches are mounted for each axis. When the stage moves to the end of its range of motion, this flag blocks the photodiode's signal to the phototransistor, and the electronics signal the stage to stop. Such a system, while similar to a rocker switch or magnetic switch involves fewer parts and greater positioning accuracy. The sample translation stage requires a large number of vacuum electrical feedthroughs (46) to control 2 motors, 4 encoders, 4 limit switches, and a thermocouple. Furthermore, because this is being mounted on an FTMS which has high sensitivity to RF interference noise, it was critical that the feedthrough and cables be well shielded. The vacuum chamber for this sample translation stage was designed to be as small as possible (for efficient pumpdown) but large enough to allow the full range of motion for the stage. The sample translation stage control was developed as a set of commands that can be called from a dynamically linked library (dll) in any Microsoft window's based programming language. The language used here was C++ as implemented in Microsoft Visual C++, version 6. A copy of the stage has now been constructed for use with the cryogenic FTMS being assembled within the BUSM Cardiovascular Proteomics Center.
这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者(PI)可能从另一个NIH来源获得了主要资金, 因此可以在其他CRISP条目中表示。所列机构为 研究中心,而研究中心不一定是研究者所在的机构。 振动冷却MALDI-FTMS的改进和优化仍在继续。 今年,我们对与弗劳恩霍夫研究所合作设计和建造的XY平台进行了进一步的测试和评估。 该阶段是MALDI-FTMS完全自动化的关键组成部分。 定义了该阶段的设计参数,以处理典型的蛋白质组学类型样品。 许多蛋白质组学实验使用的机器人被设计为与尺寸为~76 x 89 mm的微量滴定板一起工作。 因此,要求载物台具有至少100 x 100 mm的全行程。对于所使用的MALDI傅里叶变换质谱仪,从点到点的行程时间不是实验速度的限制因素,因此定义2 mm/sec的行程,因为它允许载物台在0.5秒内定位到下一个1 mm远的点。 定位精度被定义为1 μ m,这比标准MALDI实验所需的精度要高得多,在标准MALDI实验中,激光束斑的直径通常为50 μ m,但这将允许载物台在MALDI成像模式下使用。 除此之外,工作台必须是真正的真空兼容,必须垂直安装,并且不产生可检测到的RF干扰噪声,并且必须占用最小的空间,以便真空室可以尽可能小,以提高泵送性能。 二维平台是一个单一的块设计与两个轴电机,线性编码器和位置开关都集成到中央块。 该系统使用滚珠丝杠和交叉滚子轴承,这将需要在真空中进行最少的润滑;所需的少量润滑由低蒸汽压扩散泵油提供。 为了将电机安装到这个块中,它们安装在铜支架上(将其热量倾倒到充当散热器的块中),滑轮用于将其扭矩传递到滚珠丝杠。 这些滑轮的皮带实际上是小型电焊不锈钢带,为了保持它们在滑轮上居中,滑轮被磨得稍微凹进去。 将张紧固定螺钉安装到测试块中,以便在测试期间正确拧紧滑轮。 为了防止过度确定滚珠丝杠的位置,它只安装在两个地方:滑轮轴承组件和板。 舞台本身设计成三块,一块上下板和中央块。 电机、限位开关和线性编码器电子设备都安装在中央块上(并散热)。 线性编码器滑块安装在上下板上。 这三块经过黑色阳极氧化处理,以提高表面硬度,但更重要的是,以改善块的辐射冷却。 所有导线布线也包含在块中。 电机和电子设备将其热负荷倾倒到中央块中,中央块只能通过轴承处的传导冷却和辐射冷却来散热。 传导冷却是非常低效的,因为轴承是不锈钢的(非常低的传热能力),接触面积非常小。 考虑了铜带或编织物以提高传导传热率,但是与辐射冷却率相比,这种带的传热的快速计算表明通过铜带的传热可以忽略不计。 有几个定位螺钉用于交叉滚柱轴承、导线迹线和用于安装电机、轴承和电子设备的螺栓孔。 所有这些孔都是通风的,以尽量减少虚拟泄漏。 该系统使用四个位置编码器,两个线性编码器用于确定位置,两个旋转编码器安装在电机背面用于确定速度。 该系统使用光学限位开关。 开关是安装在中央台体上的光电二极管/光电晶体管对,其由安装在上下台板上的铝标志中断。 每个轴安装两个这样的开关。 当载物台移动到其运动范围的末端时,该标志阻止光电二极管向光电晶体管发送信号,并且电子设备向载物台发送信号以停止。 这种系统虽然类似于摇臂开关或磁性开关,但涉及更少的部件和更高的定位精度。 样品平移台需要大量的真空电馈电器(46)来控制2个电机、4个编码器、4个限位开关和一个热电偶。 此外,由于它安装在对RF干扰噪声具有高灵敏度的FTMS上,因此馈通和电缆必须得到良好的屏蔽。 用于该样品平移台的真空室被设计为尽可能小(用于有效抽气),但足够大以允许台的全范围运动。 示例转换阶段控件是作为一组命令开发的,可以从任何基于Microsoft窗口的编程语言的动态链接库(dll)中调用。 这里使用的语言是在Microsoft Visual C++版本6中实现的C++。该阶段的副本现已构建,用于与BUSM心血管蛋白质组学中心内组装的低温FTMS一起使用。

项目成果

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PETER B. O'CONNOR其他文献

PETER B. O'CONNOR的其他文献

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{{ truncateString('PETER B. O'CONNOR', 18)}}的其他基金

FTMS SYSTEM UPGRADES
FTMS 系统升级
  • 批准号:
    7955883
  • 财政年份:
    2009
  • 资助金额:
    $ 3.88万
  • 项目类别:
USE OF 18O LABELS TO MONITOR DEAMIDATION DURING SAMPLE PROCESSING
使用 18O 标签监测样品处理过程中的脱酰胺作用
  • 批准号:
    7955974
  • 财政年份:
    2009
  • 资助金额:
    $ 3.88万
  • 项目类别:
DEVELOPMENT OF AN AMPLITUDE AND FREQUENCY STABILIZED HIGH POWER OSCILLATOR
稳幅稳频大功率振荡器的研制
  • 批准号:
    7955976
  • 财政年份:
    2009
  • 资助金额:
    $ 3.88万
  • 项目类别:
IMPROVED PREAMPLIFIER FOR FTICRMS
改进的 FTICRMS 前置放大器
  • 批准号:
    7955923
  • 财政年份:
    2009
  • 资助金额:
    $ 3.88万
  • 项目类别:
ARTIFACTS IN FOURIER TRANSFORM MASS SPECTROMETRY
傅里叶变换质谱中的伪影
  • 批准号:
    7955973
  • 财政年份:
    2009
  • 资助金额:
    $ 3.88万
  • 项目类别:
DOUBLE RESONANCE ECD
双共振ECD
  • 批准号:
    7955943
  • 财政年份:
    2009
  • 资助金额:
    $ 3.88万
  • 项目类别:
THE EFFECT OF FIXED CHARGE MODIFICATION ON ECD
固定收费修改对 ECD 的影响
  • 批准号:
    7955975
  • 财政年份:
    2009
  • 资助金额:
    $ 3.88万
  • 项目类别:
DIFFERENTIATION OF ISOMERIC AMINO ACID RESIDUES IN PEPTIDES USING ECD
使用 ECD 区分肽中的异构氨基酸残基
  • 批准号:
    7955921
  • 财政年份:
    2009
  • 资助金额:
    $ 3.88万
  • 项目类别:
ECD AND EDD OF NATIVE AND PERMETHYLATED GLYCANS
天然和全甲基化聚糖的 ECD 和 EDD
  • 批准号:
    7955963
  • 财政年份:
    2009
  • 资助金额:
    $ 3.88万
  • 项目类别:
TESTING APPLICATION OF THE FILTER DIAGONALIZATION METHOD TO FTMS
滤波器对角化方法在FTMS中的测试应用
  • 批准号:
    7955922
  • 财政年份:
    2009
  • 资助金额:
    $ 3.88万
  • 项目类别:

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