Epithelial CD23 in Cow Milk Allergy:Role in Pathogenesis and Function as a Diseas

牛奶过敏中的上皮 CD23：在疾病发病机制和功能中的作用

• 批准号: 7976571
• 负责人: Maria CECILIA BERIN
• 金额: $ 30.46万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2013-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7976571
• 关键词: Affect; Allergens; Allergic; Anaphylaxis; Antigens; Appearance; Binding; Biological Markers; Cattle; Cell Line; Clinical; Complex; Disease; Effector Cell; Epithelial; Epithelial Cells; Epithelium; Feces; Food; Food Hypersensitivity; Functional disorder; Gastrointestinal tract structure; Human; Hypersensitivity; IgE; Immunologics; Immunotherapy; Individual; Inflammation; Inflammatory Response; Intestines; Low affinity IgE receptor; Lung; Measures; Mediating; Milk; Milk Hypersensitivity; Oral; Pathogenesis; Physiology; Role; Sampling; Series; Signal Transduction; Skin; Testing; Transgenic Mice; Xolair; base; disease natural history; food allergen; in vivo; mast cell; omalizumab; research study; response; trafficking; uptake

In order for food allergens to initiate an allergic response throughout the body, they must first traffic across the single layer of columnar epithelial cells that line the gastrointestinal tract. We have recently shown that a facilitated antigen sampling mechanism occurs whereby IgE in the intestinal lumen binds to antigens and can be trafficked as a complex across the epithelium by the low-affinity IgE receptor CD23. These antigen-lgE complexes can then act of effector cells such as mast cells, leading to degranulation and alteration of normal physiology of the gut, lung, or skin. In addition, we have shown that subjects with food allergy have detectable CD23 and food-specific IgE levels in the stool, which non-atopic controls do not. We hypothesize that the CD23-mediated uptake mechanism is a critical step in the pathophysiology of food allergy, and appearance of CD23 and IgE in the stool may be a useful non-invasive biomarker of food allergic disease. In these proposed experiments, we will examine the use of stool CD23 as a biomarker in food allergy. We will measure stool CD23 in a group of 110 milk-sensitized individuals and determine if stool CD23 is associated with clinical reactivity to milk. We will follow this group of milk-sensitized individuals longitudinally and determine the association of stool CD23 in the natural history of the disease. And finally, we will determine if oral immunotherapy with or without Xolair (omalizumab) affects the level of stool CD23. In the next series of experiments, we will determine the role of epithelial CD23 in the pathophysiology of experimental food allergy. We have shown that triggering of CD23 leads to an inflammatory response by human intestinal epithelial cells, and we will determine the signaling mechanisms responsible for this activation. In addition, we will construct a triple transgenic mouse expressing human CD23, human FcDRI, and human IgE to test the role of CD23 in antigen sampling, anaphylaxis, and allergen-induced inflammation in vivo.

为了使食物过敏原在全身引发过敏反应，它们必须首先穿越