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Massively Parallel Brain Imaging

大规模并行脑成像

基本信息

批准号：
7683010
负责人：
MARK J SCHNITZER
金额：
$ 79万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-30 至 2012-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7683010
关键词：
Ablation Adult Affect Algorithms Alzheimer&apos s Disease Alzheimer&apos s disease model Amplifiers Animal Behavior Animal Model Animals Area Award Axon Back Behavior Behavior Disorders Behavioral Biological Models Biology Biomedical Engineering Biomedical Research Biomedical Technology Biotechnology Brain Brain Diseases Brain imaging Budgets Caenorhabditis elegans Caliber Cell physiology Cells Characteristics Childhood Clinic Cocaine Collaborations Communities Complement Computer Simulation Computer Vision Systems Computer-Aided Design Cornea Crack Cocaine Data Data Analyses Data Set Data Storage and Retrieval Dendrites Deposition Development Developmental Biology Devices Disease Dissection Doctor of Philosophy Drosophila genus Drosophila melanogaster Electrical Engineering Electronics Elements Embryo Engineering Ensure Event Exhibits Face Failure Fiber Optics Figs - dietary Five-Year Plans Fluorescence Microscopy Foundations Functional Magnetic Resonance Imaging Funding Genome Genomics Goals Grant Green Fluorescent Proteins Growth Hand Head Heart Heating Hour Housing Human Human Resources Huntington Disease Image Imaging Techniques Imaging technology Individual Industry Invertebrates Knowledge Label Laboratories Larva Laser Scanning Microscopy Lasers Lead Learning Left Libraries Life Light Lobe Magnetic Resonance Mammals Manuals Mechanics Mediating Medical Methods Microscope Microscopy Modeling Motion Motor Neurons Movement Disorders Mus Nature Nematoda Nerve Degeneration Neurobiology Neurodegenerative Disorders Neurons Neurosciences Nucleotides Olfactory Receptor Neurons Operating Rooms Operative Surgical Procedures Ophthalmology Optics Outcome Paper Parkinson Disease Pathology Pattern Penetration Pharmacologic Substance Photoreceptors Physics Physiologic pulse Physiological Pigments Preclinical Drug Evaluation Principal Investigator Process Property Proteins Proteomics Publishing Reporter Research Research Personnel Resolution Resources Retina Risk Role Rotation Route Safety Sapphire Scanning Schools Science Screening procedure Senile Plaques Shapes Silicon Societies Solutions Specific qualifier value Specimen Speed Stimulus Study models Surface Surgeon Symptoms Synapses Synchrotrons Syndrome System Systems Biology Tea Techniques Technology Testing Textbooks Time Tissues Training Triplet Multiple Birth Tube United States National Academy of Sciences United States National Institutes of Health Universities Variant Veterans Virion Vision Visual Visual Fields Visual Motion Visual system structure Work Zebrafish addiction age related base behavioral sensitization brain volume career cellular imaging cocaine exposure commercialization computer science cost data acquisition design developmental disease developmental neurobiology digital dopamine system dopaminergic neuron drug discovery essays experience fluorescence imaging fluorescence microscope fly genetic manipulation genetic strain high throughput screening high throughput technology in vivo innovation insight instrument instrumentation lens light microscopy light scattering meetings member minimally invasive mouse genome mutant nanoscale nervous system disorder neural circuit neural patterning neurogenetics novel novel therapeutics object motion optical imaging optical traps practical application prevent professor programs reconstruction regenerative relating to nervous system research study response selective expression sensory stimulus sensory system structural biology success tool two-photon

项目摘要

I propose a disruptive technology that will revolutionize our understanding of brain function, development, and disease. Because the study of neural circuits remains deeply limited by a paucity of data, we need massively parallel approaches to brain imaging that will raise data acquisition rates by over two orders of magnitude. High-throughput technologies have already revolutionized certain areas of biology such as genomics and proteomics, but neuroscience has yet to experience a growth spurt of comparable magnitude. I will construct instrumentation allowing the brain volumes of ~100 alert flies to be imaged simultaneously by two-photon fluorescence microscopy. I have chosen the fruit fly, Drosophila melanogaster, because of its small brain, its sophisticated behavioral repertoire, the large number of strains with genetically targeted alterations to brain circuitry, the utility of fluorescence imaging of neural activity in this species, and the importance of the fly as a model for the study of many brain diseases. Massively parallel brain imaging will open entirely new avenues: 1) The ability to track neural dynamics across the brains of large numbers of normal flies and those with genetically induced neural circuit perturbations will revolutionize our understanding of how neural circuits produce animal behavior; 2) The now prominent role of the fruit fly as a model system for the study of developmental disorders, neurodegenerative diseases, and addiction implies we will gain significant medical insights into devastating conditions; 3) Our technology will have important applications to drug screening, allowing the cellular effects of new compounds to be assessed rapidly in vivo; 4) The ability to perform high-throughput time-lapse imaging of cellular events during the maturation of fly embryos will allow an additional revolution in developmental neurobiology. Applications of our technology will also be plentiful in other model organisms such as nematodes and zebrafish, profoundly impacting multiple areas of biomedicine.

我提出了一种颠覆性的技术，它将彻底改变我们对大脑功能的理解，