Biomimetic Properties of Cementum and Its Interfaces

牙骨质及其界面的仿生特性

• 批准号: 7639786
• 负责人: Sunita P Ho
• 金额: $ 24.9万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7639786
• 关键词: Accounting; Address; Age; Animal Model; Anterior; Apical; Area; Arts; Atomic Force Microscopy; Award; Binding; Biocompatible Materials; Biological; Biomechanics; Biomedical Engineering; Biomimetics; Bone Resorption; Cadaver; Cell Adhesion; Cell Proliferation; Cell division; Cervix Uteri; Characteristics; Chemical Structure; Chemicals; Chondroitin ABC Lyase; Chondroitin Sulfate A; Chondroitin Sulfate C; Collagen; Collagen Fibril; Collagen Type I; Complex; Connective Tissue; Cytoskeleton; Data; Data Collection; Dehydration; Dental; Dental Cementum; Dental Enamel; Dentin; Dermatan Sulfate; Disease; Disease Progression; Engineering; Environment; Enzymes; Facility Construction Funding Category; Faculty; Female; Gender; Glycosaminoglycans; Goals; Guidelines; Hardness; Human; Hydration status; Image; Incisor; Inflammation; Interferometry; Investigation; Journals; Keratan Sulfate; Knowledge; Location; Manuscripts; Maps; Matrix Metalloproteinases; Measures; Mechanics; Memory; Mentors; Microscopy; Minerals; Modeling; Molar tooth; Natural regeneration; Object Attachment; Pathway interactions; Periodontal Diseases; Periodontal Ligament; Periodontitis; Periodontium; Phase; Plant Roots; Procedures; Property; Proteoglycan; Publishing; Purpose; Rattus; Research; Research Personnel; Shapes; Shock; Specimen; Structure; Techniques; Testing; Time; Tissue Engineering; Tissues; Tooth Loss; Tooth structure; United States National Institutes of Health; Variant; Width; Work; age group; alveolar bone; animal tissue; base; bone; cell behavior; cell growth; chondroitinase B; crosslink; desire; hydrophilicity; keratan-sulfate endo-1,4-b-galactosidase; male; member; nanoscale; next generation; novel; oral tissue; programs; pyridinoline; response; scaffold; tissue regeneration

The overall purpose of the NIH Pathway to Independence Award K99/ROO application is to provide a year of support for the mentored phase that is crucial in completing the critical testing of four hypotheses, followed by manuscript submission to relevant journals. The subsequent 3 year independent phase is necessary to establish my research program and reaching my goals as a junior faculty member. It would enable me to establish myself as an independent investigator in biomaterials integration and characterization taking into account both human and comparable animal tissues, subsequently building tissues with well optimized bioengineered interfaces. This award will enable me to study the proposed specific aims defined in this proposal and expand my knowledge on building the next generation materials for tissue regeneration. The thrust of these efforts is based on a general hypothesis that the functional biomechanics of a tooth are derived from structural and chemical interactions of its different components at several hierarchical levels (macro-, micro- and nano-scales), and that these yield properties that depend on location, age, and gender. Destruction of tissues including the periodontal ligament (PDL), cementum, and bone can cause loss of teeth due to periodontitis. Key challenges include 1). Understanding degradation of the tissues associated with disease progression, and 2). Regeneration of the interfaces that bind the oral tissues together. To address the first challenge, an animal model for periodontitis will be developed and the sequential degeneration of tissues determined by studying their structure, chemical composition and mechanical properties. For the second challenge, tissue engineering (TE) can be used to create novel scaffolds. However, a major limitation of current TE procedures is limited knowledge of scaffold biomechanics. An efficient scaffold should sustain functional loads in addition to providing an environment conducive to desired cell behavior. To address these challenges, the inherent characteristics of the tissues and their interfaces must be determined. Then, they can be mimicked using TE to create appropriate scaffolds. Hence the following specific aims are defined to: 1). Investigate structure, chemical composition, mechanical properties of primary and secondary cementums, 2). Investigate structure, chemical composition, mechanical properties of cementum and its interface with root dentin, 3). Investigate strain fields using functional loads on anterior and posterior teeth as a function of age, 4). Perform a comparison studies between structure, chemical composition and mechanical properties of healthy human and rat alveolar bone, PDL, cementum, root dentin and their bimaterial interfaces. This information will be used to determine chronological changes in structure, chemical composition and mechanical properties in the rat periodontal tissues and their bimaterial interfaces during disease progression.

NIH 独立之路奖 K99/ROO 申请的总体目的是提供 对指导阶段的支持一年，这对于完成四个假设的关键测试至关重要， 然后将稿件提交给相关期刊。接下来的 3 年独立阶段是 对于建立我的研究计划和实现我作为初级教员的目标是必要的。它会 使我能够成为生物材料整合和表征领域的独立研究者 考虑到人类和类似的动物组织，随后构建具有良好性能的组织 优化的生物工程界面。该奖项将使我能够研究拟议的具体目标 在这个提案中，并扩展了我关于构建下一代组织再生材料的知识。 这些努力的主旨是基于一个一般假设，即牙齿的功能生物力学是 源自其不同成分在多个层次上的结构和化学相互作用 （宏观、微观和纳米尺度），并且这些产生的特性取决于位置、年龄和性别。 包括牙周膜 (PDL)、牙骨质和骨在内的组织的破坏会导致牙周组织丧失 牙齿由于牙周炎。主要挑战包括 1)。了解相关组织的退化 随着疾病进展，2)。将口腔组织粘合在一起的界面的再生。到 解决第一个挑战，将开发牙周炎动物模型，并进行后续研究 通过研究组织的结构、化学成分和力学来确定组织的退化 特性。对于第二个挑战，组织工程（TE）可用于创建新型支架。 然而，当前 TE 程序的一个主要限制是支架生物力学知识有限。一个 有效的脚手架除了提供有利于所需的环境外，还应承受功能负载 细胞行为。为了应对这些挑战，组织及其界面的固有特性 必须确定。然后，可以使用 TE 模仿它们来创建合适的支架。因此 确定了以下具体目标： 1).研究结构、化学成分、机械性能 初级牙骨质和次级牙骨质的特性，2)。研究结构、化学成分、 牙骨质及其与牙根牙本质界面的机械性能，3)。使用研究应变场 前牙和后牙的功能负荷与年龄的关系，4)。进行比较研究 健康人和大鼠牙槽骨的结构、化学成分和机械性能之间的关系， PDL、牙骨质、根牙本质及其双材料界面。该信息将用于确定 大鼠牙周结构、化学成分和机械性能的时间变化 疾病进展期间的组织及其双材料界面。