Infrared microscopy for a systems approach to prostate pathology

描述（由申请人提供）：前列腺医疗保健迫切需要能够提供新的预后信息和/或帮助做出准确、客观和自动化决策的技术，以帮助病理学家。该项目的主要目标是提供一种临床和研究使用的化学成像仪器，可由病理实验室中任何受过培训的人员操作。首先，将开发用于高分辨率傅里叶变换红外（FT-IR）光谱成像的新仪器和分析方法。其次，将量化缓慢和快速生长肿瘤的特征性结构（形态学）和生化转化。第三，将开发分析方法，以将提取的信息与肿瘤的侵袭潜力相关联。预测结果将与今天的临床标准（Komplogram）进行比较。一个系统的分析方法，提出了检查重要的组织学和化学转化的特点侵略性病变。新的生化见解，预计从这些模型，将导致最终解释为什么我们能够准确地定义侵略性病变。如果该项目成功，它有可能改变患者的治疗决定，并改变研究组织学评估的标准实践。 公共卫生相关性：该项目的主要目标是利用新的化学成像技术来识别那些真正有死于前列腺癌风险的人。为了实现这一目标，我们开发了新的化学成像技术，可以在不使用染料或标记的情况下测量组织的空间和分子含量。将开发分析方法以从测量中提取信息并将其与前列腺的生化知识相关联。最后，提出了一种系统病理学方法来预测那些处于危险之中的人。该项目有可能通过准确识别侵袭性和惰性病变来改变患者的治疗决策。这将使资源集中在约40，000名患有致命疾病的患者身上，并避免在约200，000人身上浪费开支。如果成功，仪器和分析方法的建立将改变前列腺癌临床组织学评估研究的标准实践。

DESCRIPTION (provided by applicant): Technologies that can provide new prognostic information and/or help make accurate, objective and automated decisions that aid the pathologist are sorely needed in prostate healthcare. The major goal of this project is to provide a chemical imaging instrument for clinical and research use that can be operated by any trained person in pathology laboratories. First, novel instrumentation and analytical methods for high-resolution Fourier transform infrared (FT-IR) spectroscopic imaging will be developed. Second, both structural (morphologic) and biochemical transformations that are characteristic of slow and rapidly growing tumors will be quantified. Third, analytical methods will be developed to relate the extracted information to aggressive potential of the tumor. Predictions will be compared to the clinical standard today (Kattan nomogram). A systems analysis approach is proposed to examine important histologic and chemical transformations that characterize aggressive lesions. New biochemical insight is expected from these models that will lead ultimately to an explanation of why we are able to define aggressive lesions accurately. If the project is successful, it has the potential to transform therapy decisions for patients and alter the standard practice in histologic assessment of research. PUBLIC HEALTH RELEVANCE: The major goal of this project is to leverage novel chemical imaging technology to identify those truly at risk of death from prostate cancer. To achieve this goal, we develop novel chemical imaging technology that can measure the spatial and molecular content of tissue as without the use of dyes or labels. Analytical methods will be developed to extract information from the measurements and relate it to biochemical knowledge of the prostate. A systems pathology approach, finally, is presented to predict those at risk. The project has the potential to transform therapy decisions for patients by identifying aggressive and indolent lesions accurately. This would enable focusing of resources on ~40,000 patients who have lethal disease and avoid wasteful expenditures on ~200,000 people. If successful, establishment of the instrumentation and analytical methods here would alter the standard practice in clinical histologic assessment of research in prostate cancer.