Infrared microscopy for a systems approach to prostate pathology

描述(由申请人提供)：能够提供新的预后信息和/或帮助做出准确、客观和自动化的决定以帮助病理学家的技术在前列腺保健中是迫切需要的。该项目的主要目标是提供一种临床和研究使用的化学成像仪器，可以由病理实验室中任何训练有素的人员操作。首先，将开发用于高分辨率傅里叶变换红外(FT-IR)光谱成像的新仪器和分析方法。其次，以肿瘤生长缓慢和快速生长为特征的结构(形态)和生化转变都将被量化。第三，将开发分析方法，将提取的信息与肿瘤的侵袭潜力联系起来。预测将与今天的临床标准(卡坦诺模图)进行比较。提出了一种系统分析的方法来检查侵袭性病变的重要组织学和化学转化。预计从这些模型中可以获得新的生化见解，最终将解释为什么我们能够准确地定义侵袭性病变。如果该项目成功，它有可能改变患者的治疗决定，并改变组织学研究评估的标准做法。 公共卫生相关性：该项目的主要目标是利用新的化学成像技术来识别那些真正面临前列腺癌死亡风险的人。为了实现这一目标，我们开发了新的化学成像技术，可以在不使用染料或标记的情况下测量组织的空间和分子含量。将开发分析方法，从测量中提取信息，并将其与前列腺的生化知识联系起来。最后，提出了一种预测风险人群的系统病理学方法。该项目有可能通过准确识别侵袭性和惰性病变来改变患者的治疗决定。这将使资源集中在约40,000名患有致命疾病的患者身上，并避免向约200,000人支付浪费性支出。如果成功，这里仪器和分析方法的建立将改变前列腺癌研究的临床组织学评估的标准做法。

DESCRIPTION (provided by applicant): Technologies that can provide new prognostic information and/or help make accurate, objective and automated decisions that aid the pathologist are sorely needed in prostate healthcare. The major goal of this project is to provide a chemical imaging instrument for clinical and research use that can be operated by any trained person in pathology laboratories. First, novel instrumentation and analytical methods for high-resolution Fourier transform infrared (FT-IR) spectroscopic imaging will be developed. Second, both structural (morphologic) and biochemical transformations that are characteristic of slow and rapidly growing tumors will be quantified. Third, analytical methods will be developed to relate the extracted information to aggressive potential of the tumor. Predictions will be compared to the clinical standard today (Kattan nomogram). A systems analysis approach is proposed to examine important histologic and chemical transformations that characterize aggressive lesions. New biochemical insight is expected from these models that will lead ultimately to an explanation of why we are able to define aggressive lesions accurately. If the project is successful, it has the potential to transform therapy decisions for patients and alter the standard practice in histologic assessment of research. PUBLIC HEALTH RELEVANCE: The major goal of this project is to leverage novel chemical imaging technology to identify those truly at risk of death from prostate cancer. To achieve this goal, we develop novel chemical imaging technology that can measure the spatial and molecular content of tissue as without the use of dyes or labels. Analytical methods will be developed to extract information from the measurements and relate it to biochemical knowledge of the prostate. A systems pathology approach, finally, is presented to predict those at risk. The project has the potential to transform therapy decisions for patients by identifying aggressive and indolent lesions accurately. This would enable focusing of resources on ~40,000 patients who have lethal disease and avoid wasteful expenditures on ~200,000 people. If successful, establishment of the instrumentation and analytical methods here would alter the standard practice in clinical histologic assessment of research in prostate cancer.